There were two main experiments in the (-)epi/myostatin/follistatin study - mice and humans.
The mouse study used 1mg/kg twice per day orally. The mice were treated for 2 weeks.
"recognized modulators of SkM growth (myostatin, follistatin) [8], senescenceassociated
β-galactosidase (SA-β-Gal) [9] and myogenic differentiation
(myogenin, MyoD, MEF2A, Myf5) [10] and report on the effects of Epi treatment
in young (6 month old, n=5/group) and old (26 month, n=5/group) C57BL/6 male
mice. For these studies, quadriceps muscle samples were obtained from mice
treated for 2 weeks with either vehicle by gavage (water) or Epi (1 mg/kg BID)
and immediately frozen. Studies in animals were approved by UCSD’s IACUC.
Human studies. Frozen, quadriceps SkM samples were obtained from a human
biopsy tissue bank and were used to compare the same markers as above, in
normal young (28.5±7 years, n=6) vs. old (62±2 years, n=6) subjects. An initial,
pilot study was also performed in human subjects (n=6, average age 41±5 years
and weight 77.4±7.7 Kg) to assess the effects of Epi treatment on muscle
strength and plasma levels of myostatin and follistatin. Plasma myostatin and
follistatin levels can change in association with those in muscle in response to,
for example, exercise [11,12] and were thus, used as a surrogate for possible
responses occurring in SkM. Human subjects were treated for 7 days with 25 mg
of pure Epi (Sigma-Aldrich) provided in capsules BID (~1 mg/kg/day)."
The human portion used 25mg twice per day and dramatic results were obtained after 7 days.
Trying to figure out how to host the full study .pdf for you guys.