"thus revealing potential deleterious effects of AA on human skeletal muscle cell health and viability."
Can anyone explain this study. Conclusion said it causes damage to muscle cell health?
the authors:
“However, our findings that supraphysiological doses of AA are detrimental to skeletal muscle are not parallel to any study in humans.”
this is a “test tube” study of human skeletal muscle cell *myogenesis tested against ArA. The doses were purposely supraphysiological to increase the “signal to noise” ratio. The results, even at low (applied quantifier; not supplemented) doses were the reverse of those seen when tested against mouse and bovine skeletal muscle.
take always:
1) the concentration of ArA that reaches skeletal muscle after supplementation is completely unknown
“…since it is unknown what this translates to in current human papers but covers a broad spectrum of doses.”
2) adult myogenesis certainly occurs in adults but is of much lesser importance then late embryonic development
3) the study results were the opposite of what was anticipated and ha not been replicated. The methods the authors used were also different then previous authors
“It is unclear whether our results are due to the unique experimental conditions and cell lines used compared to others.”
4) study reinforced the need to stay away from COX2 inhibitors
“these data and our recent findings indicating a COX-2 inhibitor (i.e. celecoxib), but not a COX-1 inhibitor (NS-398), is detrimental to skeletal muscle cells are the rationale for not testing a COX-1 inhibitor in tandem with AA”
anyway, the new research on the SM hypertrophic mechanisms of ArA appear to be largely mediated through its downstream, LOX derived, products, rather than prostaglandins
