1.) For those of you who are wondering, Rostam is purposely trying to start **** because of an argument we had regarding Vicaine a couple years ago. He thought selling the product was unethical and also posted some inaccurate information regarding the product's effects. Obviously, I did not share his opinion...and in response to the inaccurate info he posted, I put up some study links showing he was wrong. He did not like that and as a result, he became an even bigger cock, so I eventually got irritated and dug into him for it. Since then he has followed me around attempting to discredit me anywhere he can (he hasn't succeeded).
2.) Rostam's current gripe is in regards to Insuligen and has to do with a claim I put forth in my product description. In this claim I drew from a clinical study performed at King's College, in which I referenced a particular ingredient's (GymnePURE) ability to induce Insuligen secretion without damaging beta cell wall integrity (unlike other gymnema products). Due to being extremely busy, I told him that if he wanted to validate that particular claim (which was very minor and had nothing to do with the product's effectiveness), he would have to look it up himself-- a discussion he appears to have forgotten. Now, if a member were to ask a sincere question in an innocent manner, I would have no problem spending my time in an effort to help them, but when someone has zero intention of spending a single penny on the product and is just trying to start ****, I typically don't waste my time on such individuals (i.e. Rostam).
3.) With that said, since Rostam continues to try and cause problems (despite me previously telling him that if he wanted any info he would have to find it himself), I feel like I have no choice buy to search for the study links. However, I will say this. If Rostam EVER attempts to discredit any aspect of any product ever again, without any evidence to back up his claims, I will seek a perm ban from the Admin.
Before moving onto the studies below, allow me to cover a few things first. To re-cap the issue, the makers of GymnePURE (the specific type of gymnema I use in Insuligen) performed a study showing that it does not damage/kill beta cells walls when used at effective human doses. The same is not true of regular gymnema extracts. In other words, regular extracts induce insulin release by causing an increase in beta cell wall permeability (bad), while GymnePURE causes insulin release by stimulating insulin exocytosis (good).
The first study posted below pertains to GymnePURE (also known as OSA), while the second study pertains to a differnet gymnema extract known as GS4, which stimulates insulin secretion in the same manner as regular gymnema extracts. In the second study you will see the term "trypan blue uptake". The reason the researchers are measuring trypan blue uptake is because it indicates the presence of beta cell wall damage/death. Notice that GymnePURE avoids this deleterious effect, while normal Gymnema does not.
In conclusion, GymnePURE (OSA; a high molecular weight form of gymnema) has been studied extensively and is widely regarded as the most effective and safest form of Gymnema available today. Information regarding GymnePURE is very easy to find on PubMed, for those of you who may be interested in diving further into the details. See blue highligthed text below. Thank you.
Characterisation of the insulinotropic activity of an aqueous extract of Gymnema sylvestre in mouse beta-cells and human islets of Langerhans.
Liu B1,
Asare-Anane H,
Al-Romaiyan A,
Huang G,
Amiel SA,
Jones PM,
Persaud SJ.
Author information
Abstract
Leaves of the Gymnema sylvestre (GS) plant have been used to treat diabetes mellitus for millennia, but the previously documented insulin secretagogue effects of GS extracts in vitro may be non-physiological through damage to the beta-cells.
We have now examined the effects of a novel GS extract (termed OSA) on insulin secretion from the MIN6 beta-cell line and isolated human islets of Langerhans. Insulin secretion from MIN6 cells was stimulated by OSA in a concentration-dependent manner, with low concentrations (0.06-0.25 mg/ml) having no deleterious effects on MIN6 cell viability, while higher concentrations (> or = 0.5 mg/ml) caused increased Trypan blue uptake. OSA increased beta-cell Ca2+ levels, an effect that was mediated by Ca2+ influx through voltage-operated calcium channels. OSA also reversibly stimulated insulin secretion from isolated human islets and its insulin secretagogue effects in MIN6 cells and human islets were partially dependent on the presence of extracellular Ca2+.
These data indicate that low concentrations of the GS isolate OSA stimulate insulin secretion in vitro, at least in part as a consequence of Ca2+ influx, without compromising beta-cell viability. Identification of the component of the OSA extract that stimulates regulated insulin exocytosis, and further investigation of its mode(s) of action, may provide promising lead targets for Type 2 diabetes therapy.
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Gymnema sylvestre stimulates insulin release in vitro by increased membrane permeability.
Persaud SJ1,
Al-Majed H,
Raman A,
Jones PM.
Author information
Abstract
To determine whether extracts of Gymnema sylvestre may have therapeutic potential for the treatment of non-insulin-dependent diabetes mellitus (NIDDM), we examined the effects of an alcoholic extract of G. sylvestre (GS4) on insulin secretion from rat islets of Langerhans and several pancreatic beta-cell lines. GS4 stimulated insulin release from HIT-T15, MIN6 and RINm5F beta-cells and from islets in the absence of any other stimulus, and GS4-stimulated insulin secretion was inhibited in the presence of 1 mM EGTA. Blockade of voltage-operated Ca(2+) channels with 10 microM isradipine did not significantly affect GS4-induced secretion, and insulin release in response to GS4 was independent of incubation temperature.
Examination of islet and beta-cell integrity after exposure to GS4, by trypan blue exclusion, indicated that concentrations of GS4 that stimulated insulin secretion also caused increased uptake of dye. Two gymnemic acid-enriched fractions of GS4, obtained by size exclusion and silica gel chromatography, also caused increases in insulin secretion concomitant with increased trypan blue uptake. These results confirm the stimulatory effects of G. sylvestre on insulin release, but indicate that GS4 acts by increasing cell permeability, rather than by stimulating exocytosis by regulated pathways. Thus the suitability of GS4 as a potential novel treatment for NIDDM can not be assessed by direct measurements of beta-cell function in vitro.