Ask a Biochemist anything!

TheProfessor said:
From what I can tell at this point it's all anecdotal.

40-80 micrograms bilaterally pre-training on the muscle group that's going to be trained (IM). Two weeks on two weeks off. On off days pin any body part you want. Do it with a meal. If your bodyfat is low enough, a normal insulin dart should get IM. With IGF more is not better. You are more likely going to get wastage than an increased effect. So just use as much as you need. Permamant gains come much longer after usage.
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Have you seen the test results on the quality of this stuff? It's all fairly recent, but none of it is legit.
 
Resolve said:
I'm pretty sure spectroscopy is just a codeword for magic. The info you guys can pull out of a sample will boggle my mind.
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Definitely littered with ****. But it's the way she goes. I can follow individual electron transfer with femtosecond scale optical instruments.
That sort of kinetics is cool. Electrons are under appreciated, it's where all the chemistry happens!
 
TheProfessor said:
Definitely littered with ****. But it's the way she goes. I can follow individual electron transfer with femtosecond scale optical instruments.
That sort of kinetics is cool. Electrons are under appreciated, it's where all the chemistry happens!
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Yeah, see, that's what I'm talking about. 10^-15 sec??? My experiments last for days, if not weeks!
 
Resolve said:
Yeah, see, that's what I'm talking about. 10^-15 sec??? My experiments last for days, if not weeks!
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Yup. Spectroscopy is like a really bad drug. Results are NOW NOW NOW NOW and you can run dozens of experiments in a day by tweaking the parameters of the instrument(s) You get so much data you don't know what to do with it!
 
I agree. It's a good way for the newbie to learn the troubleshooting process in science. There are so many steps. So many places that things can (and do) go wrong that going back and figuring out what the problem was is more valuable than what the western blot says. Especially when high throughput sequencing and multiplexing antibody kits with barcodes are in development and or implemented.
 
Resolve said:
You in some form of Bio too?
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Pharmaceutical Science with an emphasis in Pharmacokinetics, supposedly. But it's through a biomedical science program which I'm sure is similar to how your program is set up.
 
This thread has finally served it's official purpose for me (to reach the minimum post count) but I won't argue if anyone wants to keep it alive!

-Prof
 
Hey mate

Hey guys I been bb for 6 years I have done five ab cycles now I have put on 20 of keep muscle and now at the point were I'm just not getting the results the I want from that alone I'm 26 year old 6,3 92 kg very lean would like to get to 105 kg and I have been reading crazily about igflr3 and hgh but I'm worried about all I hear of the cancer topic as I smoked for five years as a teenager silly I no would anyone no if at my age someone could already have cancer activated any advice on this or any impute would be great thanks
 
Plz help

Hey guys I been bb for 6 years I have done five ab cycles now I have put on 20 of keep muscle and now at the point were I'm just not getting the results the I want from that alone I'm 26 year old 6,3 92 kg very lean would like to get to 105 kg and I have been reading crazily about igflr3 and hgh but I'm worried about all I hear of the cancer topic as I smoked for five years as a teenager silly I no would anyone no if at my age someone could already have cancer activated any advice on this or any impute would be great thanks
 
TheDarkHalf said:
What are your thoughts on GMO food and how it will effect us on an epigenetic/nutragenomic level?
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My formal opinion is that we have been genetically modifying food for thousands of years. you ever seen what corn (just a few kernels) or strawberries (about the size of marbles) looked like before we started molesting their genetic material? Ever seen a feral pig vs a domesticated one?

Epigenetic changes aren't permanent chances in the genetic material, so that doesn't get me too excited. (maybe in expression patterns, but not the physical DNA)

GMO food is a necessary evil if the population is to keep growing the way it is. some people might not respond well (be allergic) to certain alterations in the genomes of the plants, but in reality, there are plenty of people who are allergic to foods anyway... GMO isn't where it should be, and likely wont catch up to where it needs to be either. Just like alternative energy research. Should have been completed yesterday.
 
thedonone said:
Hey guys I been bb for 6 years I have done five ab cycles now I have put on 20 of keep muscle and now at the point were I'm just not getting the results the I want from that alone I'm 26 year old 6,3 92 kg very lean would like to get to 105 kg and I have been reading crazily about igflr3 and hgh but I'm worried about all I hear of the cancer topic as I smoked for five years as a teenager silly I no would anyone no if at my age someone could already have cancer activated any advice on this or any impute would be great thanks
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I have no idea if it'll piss off any onco-genes. I'm not qualified to say. You either want the results, or you don't. If you don't want to risk your health, then live with being 92 kg. None of these compounds have long term human studies. Especially in the context of how bodybuilders abuse them.
 
TheProfessor said:
I have no idea if it'll piss off any onco-genes. I'm not qualified to say. You either want the results, or you don't. If you don't want to risk your health, then live with being 92 kg. None of these compounds have long term human studies. Especially in the context of how bodybuilders abuse them.
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A peer of mine who helped me create the Cabergolean has spent the last few years as the lead on a novel GHRH that was created as a treatment for people with AIDS whose bodies were wasting away.

What he found in some research (on cancer patients) was that when patients were given IGFBPs to negate the effects of circulating IGF that this treatment did not stop the spread of cancer (can't say which cancer); moreover, he found that although The reduction in circulating IGF slowed cancer cell proliferation, it also slowed the proliferation of lymphocytes, especially NKC. So a reduction in IGF was not the panacea.

Also, it was shown that this GHRH had the ability , unlike HGH and IGF to improve the efficacy of the thymus gland. The thymus glands performance is decimated by conventional cancer treatments, and full blown aids.

So I am absolutely not recommending you take any medications approved or unapproved, I suppose if I were you and cancer was a concern, I would see an anti aging clinician about GHRH or a supplement that increases GHRH.
 
subd to read a bunch of sh*t i dont yet understand


prof, what is your opinion on D-aspartic acid focking shat up in my brain
 
swollen87 said:
subd to read a bunch of sh*t i dont yet understand

prof, what is your opinion on D-aspartic acid focking shat up in my brain
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That is actually a really good question!
 
BigGunn said:
A peer of mine who helped me create the Cabergolean has spent the last few years as the lead on a novel GHRH that was created as a treatment for people with AIDS whose bodies were wasting away.

What he found in some research (on cancer patients) was that when patients were given IGFBPs to negate the effects of circulating IGF that this treatment did not stop the spread of cancer (can't say which cancer); moreover, he found that although The reduction in circulating IGF slowed cancer cell proliferation, it also slowed the proliferation of lymphocytes, especially NKC. So a reduction in IGF was not the panacea.

Also, it was shown that this GHRH had the ability , unlike HGH and IGF to improve the efficacy of the thymus gland. The thymus glands performance is decimated by conventional cancer treatments, and full blown aids.

So I am absolutely not recommending you take any medications approved or unapproved, I suppose if I were you and cancer was a concern, I would see an anti aging clinician about GHRH or a supplement that increases GHRH.
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This makes sense to me. To the extent that it (might) exacerbate a pre-existing condition, but not necessarily CAUSE it. And I also agree with the latter portion of the post. I would still also like to point out that this is not with healthy people looking to get an unnatural edge. The body's epigenetic profile is all ****ed up compared to a healthy athletes. It'd be interesting to directly compare the differences in the physiological roles.
 
swollen87 said:
subd to read a bunch of sh*t i dont yet understand


prof, what is your opinion on D-aspartic acid focking shat up in my brain
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Don't forget:
1. It exists in the brain and plays a vital role
2. Too much of anything is bad

Please Realize:
3. We aren't injecting it into our brains
4. I'm willing to bet that it wouldn't be realistic to consume amounts that would reach neurotoxic levels; especially the kind that tissue is being soaked in during cytotoxicity experiments.
 
"3. We aren't injecting it into our brains"

Speak for yourself!
 
Can test e injection be bad for kidney long term high dose due to the benzo and other chemical added like oil, not the hormone itself
 
What's your opinion of finasteride? And do you gave any secret topical anti androgens with no systemic absorption brewing in the lab?
 
rambo679 said:
What's your opinion of finasteride? And do you gave any secret topical anti androgens with no systemic absorption brewing in the lab?
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Finasteride: as a 5-ARI, will it help prevent male pattern baldness? Yeah. Is it an anti-androgen? Yeah.

Topical: Topical stuff is systemic by nature (albeit less than oral). You can direct how much of it may or may not be systemic based off of the vector of delivery. Whatever it is, it has to travel through vascular tissue in order to get to the target point, in which case... it becomes systemic. Injecting something where you want it is the best way to get something where you want it to go, with minimal systemic 'loss'
 
Im not sure, whatever are the usual comtents in.a vial of testE.besides the hormone also like a period of 6 minths 1gram/wk
 
Jasen said:
Im not sure, whatever are the usual comtents in.a vial of testE.besides the hormone also like a period of 6 minths 1gram/wk
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The FDA has approved use of Whatever oil is used/benzyl benzoate/and Benzoic acid for long term injectable use. I'd be more concerned with the liver having a hard time with oral compounds. Even if they are over the counter... Like tylenol.

I'm sure the lipid is metabolized like any other fat that's ingested and gets into the bloodstream, you likely even get calories from it. However, I'm not entirely sure.

Benzyl benzoate is approved as a food additive and benzoic acid is the same. I'd be more concerned with long term use affecting your cardiovascular tissue. The liver is robust in comparison.
 
I don't use orals this time, I only inject test
 
If you are just lookin to be the scariest guy in the ice cream parlor and not a high-end competitive athlete of sorts. There is really no need to do anything else...
 
Thats my belive as well, unless your cutting.extreme, theres really no need for anythimg else. If your not growing on test your doing something wrong
 
How could you identify a ribosomal gene mutation and how would you predict the type of sequence change? How could you purify the ribosomal protein and examine its sequence alteration?
 
prld2gr8ns said:
How could you identify a ribosomal gene mutation and how would you predict the type of sequence change? How could you purify the ribosomal protein and examine its sequence alteration?
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It's kind of a redundant process. we have like 40x coverage of the human genome so it's not difficult to just compare sequences. The only issue would be the crazy amount of post translational modification it might have after it's polymerized. Sometimes the actual gene is fine and that machinery might be bunk... in which case your mutation might be in a TOTALLY different area of the genome even though it effects a ribosomal protien. (chances are there will be other issues as well). The easiest way to purify a protein is to just have a bacteria work as your biosynthesis vessel. make tons of it and purify it that way. Otherwise purifying proteins from eukaryotic mitochondria is a goddamn pain.
 
TheProfessor said:
It's kind of a redundant process. we have like 40x coverage of the human genome so it's not difficult to just compare sequences. The only issue would be the crazy amount of post translational modification it might have after it's polymerized. Sometimes the actual gene is fine and that machinery might be bunk... in which case your mutation might be in a TOTALLY different area of the genome even though it effects a ribosomal protien. (chances are there will be other issues as well). The easiest way to purify a protein is to just have a bacteria work as your biosynthesis vessel. make tons of it and purify it that way. Otherwise purifying proteins from eukaryotic mitochondria is a goddamn pain.
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So would you just amplify the gene through PCR, cut it into a plasmid vector and transfect it into bacterial cells, grow and filter. Or could you just amplify it with PCR, run it through an agrose gel, cut the DNA band out and filter? I'm trying to figure out which is better for purifying and sequence analysis. For analysis of sequence alterations and predictions, I think LC-MS/MS would be my first choice. I know there are dATP radiolabeling and dye determination techniques but those just seem like a pain in the ass when your just trying to sequence. Especially compared to the ease of MS. Thoughts?
 
hey quick Q: how can one cycle possibly ruin the your endocrine system for the rest of your life. doesnt make sense to me i thought the body was always looking for homeostasis so after cycle once damage is done wouldnt it strive to reach homeostasis hormonal wise again? or am i missing something.
 
prld2gr8ns said:
So would you just amplify the gene through PCR, cut it into a plasmid vector and transfect it into bacterial cells, grow and filter. Or could you just amplify it with PCR, run it through an agrose gel, cut the DNA band out and filter? I'm trying to figure out which is better for purifying and sequence analysis. For analysis of sequence alterations and predictions, I think LC-MS/MS would be my first choice. I know there are dATP radiolabeling and dye determination techniques but those just seem like a pain in the ass when your just trying to sequence. Especially compared to the ease of MS. Thoughts?
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Those are some pretty old school methods dude. Getting a sequence through MS is a silly idea. Radiolabeling is super old school. You know the sequence of the gene. Just clone it out and submit it for sequencing. Just make sure your primers are upstream and downstream of the gene so you can catch any mutations within the gene. The only reason to clone any DNA into a bacteria is to have it express the protein. In which case you can correlate the mutation in the DNA sequence to the malfunction of the protein (unless there is post translational modification, in which case you can't use a bacteria)
 
rochabp said:
hey quick Q: how can one cycle possibly ruin the your endocrine system for the rest of your life. doesnt make sense to me i thought the body was always looking for homeostasis so after cycle once damage is done wouldnt it strive to reach homeostasis hormonal wise again? or am i missing something.
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You have to so do something seriously stupid. I'd be willing to be SOME hormones can cause permanent damage if abused and used incorrectly. Like thyroid hormone... Also using some hormones while the body is developing can cause some funky changes that wouldn't be very good... and be long term. you have to be more specific. I don't know what types of hormones you are talking about and what type of 'damage' is being caused. I don't believe in perma-shutdown of steroid hormone production...
 
prld2gr8ns said:
So would you just amplify the gene through PCR, cut it into a plasmid vector and transfect it into bacterial cells, grow and filter. Or could you just amplify it with PCR, run it through an agrose gel, cut the DNA band out and filter? I'm trying to figure out which is better for purifying and sequence analysis. For analysis of sequence alterations and predictions, I think LC-MS/MS would be my first choice. I know there are dATP radiolabeling and dye determination techniques but those just seem like a pain in the ass when your just trying to sequence. Especially compared to the ease of MS. Thoughts?
Click to expand...

clone it out and run like a 200ul PCR. Cut the beeyotch out of a gel and submit that. Make sure you use a HIGH FIDELITY enzyme. You don't want the PCR itself to introduce mutation.
 
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