Protein supplementation does not alter intramuscular anabolic signaling...

[JudoJosh]

fresh off the press

Protein supplementation does not alter intramuscular anabolic signaling or endocrine response after resistance exercise in trained men.

The mammalian/mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway appears to be the primary regulator of muscle protein synthesis. A variety of stimuli including resistance exercise, amino acids, and hormonal signals activate mTORC1 signaling. The purpose of this study was to investigate the effect of a protein supplement on mTORC1 signaling following a resistance exercise protocol designed to promote elevations in circulating hormone concentrations. We hypothesized that the protein supplement would augment the intramuscular anabolic signaling response. Ten resistance-trained men (age, 24.7 ± 3.4 years; weight, 90.1 ± 11.3 kg; height, 176.0 ± 4.9 cm) received either a placebo or a supplement containing 20 g protein, 6 g carbohydrates, and 1 g fat after high-volume, short-rest lower-body resistance exercise. Blood samples were obtained at baseline, immediately, 30 minutes, 1 hour, 2 hours, and 5 hours after exercise. Fine-needle muscle biopsies were completed at baseline, 1 hour, and 5 hours after exercise. Myoglobin, lactate dehydrogenase, and lactate concentrations were significantly elevated after resistance exercise (P < .0001); however, no differences were observed between trials. Resistance exercise also elicited a significant insulin, growth hormone, and cortisol response (P < .01); however, no differences were observed between trials for insulin-like growth factor-1, insulin, testosterone, growth hormone, or cortisol. Intramuscular anabolic signaling analysis revealed significant elevations in RPS6 phosphorylation after resistance exercise (P = .001); however, no differences were observed between trials for signaling proteins including Akt, mTOR, p70S6k, and RPS6. The endocrine response and phosphorylation status of signaling proteins within the mTORC1 pathway did not appear to be altered by ingestion of supplement after resistance exercise in resistance-trained men.

Protein supplementation does not alter intramuscular anabolic signaling or endocrine response after resistance exercise in trained men. - PubMed - NCBI

Interesting study. Wish they would have used a greater protein amount though

 

[carmaf]

So this means a post-workout shake does nothing?

 

[T-Bone]

No full text....

 

[JudoJosh]

So this means a post-workout shake does nothing?

no, we really cant draw much of any conclusion based on this alone. It is just a piece of a puzzle

All that we can really say is that consuming a postworkout shake probably has a negligible hormonal effect

 

[carmaf]

no, we really cant draw much of any conclusion based on this alone. It is just a piece of a puzzle

All that we can really say is that consuming a postworkout shake probably has a negligible hormonal effect

Ok thanks for the clarification. I have been of the understanding for a while now(and from personal experience as well) that carb timing is much more important post than protein is, and eating a solid meal before is more crucial than what comes after.