Yea it definitely needs to be refrigerated after it is mixed. I keep it right in the door of the beer fridge.
I use the 1/2” insulin pen, take the jab about an inch away from the belly button. Once you learn the optimal technique, it doesn’t hurt. You pinch some belly fat, lead with the point of the needle and go in at 45 degrees. If you do it right, you don’t even feel it.
I was doing 300iu 3x a week. That much raises my e2 and I need to take an AI if I’m not running any DHT compounds. The doc told me that is a generous dosing schedule, so if I missed a does I never noticed anything. Based on my experience, I think a lot of guys could see benefit from dosing 1x a week around 300-500iu, close to the weekend - for sexual purposes.
I took so much HCG over that past 6 month, I’m actually cycling off right now for a little while. I felt like my e2 was consistently higher than I liked, and I wasn’t getting as much benefit from taking it. I’m off it for 3 weeks now, I’m planning to start another biking cycle in the next few weeks so I probably won’t start running it again until the tail end of that cycle.