The PEGylation thread

thesinner

thesinner

Recovering AXoholic
This thread is to discuss anything and everything anyone can find about PEGylation.

I'll clue you into what the heck this is:

Poly Ethylene Glycol (PEG) is a polymer, which are large repeating chains of a molecule (typically carbon-based). The repeated chains are denoted by placing parenthesis around the repeating portion in the structure diagram:
Invalid Link Removed

So what is PEGylation?
PEGylation is prettymuch bonding an active drug or supplement to these PEG polymer chains. What happens is the PEG works like Puff Daddy's Sercurity Crew, protecting it from being "Iced" by West coast rappers, oxidative reangents, and such. This will in turn promote a greater half-life and greater bioavailability. (And yes, the things that are popping into your head as you're reading this are exactly things that it is currently being experimented with).


Some things I'm specifically trying to figure out (I appologize if I start throwing out jargon, but I work in the polymer industry):
-Is the 'active' being PEGylated being used as a filler (we often like to stick sh*t between the chains), or does it actually being linked into the polymer chain?
-I've found info on cross-linkers, but I haven't seen any cure accelerators (catalysts). Will this reaction cure up without the help of a CA?
-PEG is also a potent laxative.

If this thread has sparked you interest in PEGylation, feel free to ask me some Q's as I might be able to answer them (with the exception of the inquired info listed above). If you look up stuff and find any fun tidbits on PEGylation, feel free to stick them here. I'm all ears to hear it, and I foresee some PEGylated products coming to the market in the near future.


Here's some good reads:
Invalid Link Removed

Invalid Link Removed
 
I'm gonna try and see if my University is ocnducting any PEGylation research. I do undergrad research for a few extra $$$'s.
 
Wow. Never heard of that, but it sure looks promising! I'd like to see melanotan orally!
 
I know, it's pretty ill stuff.

Don't quote me on this (I'm not even affiliated with them) but I think one of the board sponsor's used to use this with an older set of products and may be using it with a newer product they're working on.
 
I dont quite understand the break down of this, but isnt it basically just like time released capsules (pain medication for example). And it also protects the compound from being destroyed or whatever immediately.

What type of supps would pegylation be useful for?
 
Travis said:
I dont quite understand the break down of this, but isnt it basically just like time released capsules (pain medication for example). And it also protects the compound from being destroyed or whatever immediately.

What type of supps would pegylation be useful for?
Click to expand...

According to what I am reading, pretty much anything...
 
Travis said:
I dont quite understand the break down of this, but isnt it basically just like time released capsules (pain medication for example). And it also protects the compound from being destroyed or whatever immediately.

What type of supps would pegylation be useful for?
Click to expand...

A lot of supplements would fall into this category. PEGylation drastically increases bioavailability in poorly bioavailable compounds.
 
Great thread. You may have already come across it but there are some people who have been dosing their resveratrol with low dose miralax (PEG 3350) to help dissolve the res, increase the surface area, and in turn increase the absorption of it. Forgive me if my wording is incorrect. Do you have any thoughts on this?
 
riseboi said:
Great thread. You may have already come across it but there are some people who have been dosing their resveratrol with low dose miralax (PEG 3350) to help dissolve the res, increase the surface area, and in turn increase the absorption of it. Forgive me if my wording is incorrect. Do you have any thoughts on this?
Click to expand...

actually, resveratrol has ueber fantastic absorption. It just gets destroyed like a Klansman waltzing through South Central at 1am. I'm wondering, though, if you're taking with Miralax, if that would kill absorption because your taking with a very strong laxative. I'm scared this might make "Resvera-turds".

I would think PEGylation would DECREASE surface area since it's now being stuck inside a chain as opposed to being a fine powder.

but as far as for use in resveratrol "You are correct, sir!" (Ed McMahon voice), that's one thing that's being looked into.
 
thesinner said:
actually, resveratrol has ueber fantastic absorption. It just gets destroyed like a Klansman waltzing through South Central at 1am. I'm wondering, though, if you're taking with Miralax, if that would kill absorption because your taking with a very strong laxative. I'm scared this might make "Resvera-turds".

I would think PEGylation would DECREASE surface area since it's now being stuck inside a chain as opposed to being a fine powder.

but as far as for use in resveratrol "You are correct, sir!" (Ed McMahon voice), that's one thing that's being looked into.
Click to expand...

Hmmmm. you're probably correct, my technical jargon is horrible to say the least. :) I''ll try and dig up the posts where people cited reasons for doing this. Their explanations are probably more sound than mine.

Personally, I haven't begun taking res with miralax yet. Right now, I only take low doses from NOW, Source Naturals, etc, but I plan to in the future with 99% res, or if I can find a synthtetic source with reasonable pricing, not likely though. Anyways, from the posts that I've read, most people who are doing this are following a 1:1 ratio of 98/99% res and miralax. I believe the normal prescribed dose per serving of miralax is @ 7grams. I don't think any of them have experienced the turds since the dose is so low; at least not anymore than those who are taking 50% res with high emodin content.
 
thesinner said:
This thread is to discuss anything and everything anyone can find about PEGylation.

I'll clue you into what the heck this is:

Poly Ethylene Glycol (PEG) is a monomer used for the preparation of polymers, which are large repeating chains of a molecule (typically carbon-based). The repeated chains are denoted by placing parenthesis around the repeating portion in the structure diagram:
Invalid Link Removed

So what is PEGylation?
PEGylation is prettymuch bonding an active drug or supplement to these PEG polymer chains. What happens is the PEG works like Puff Daddy's Sercurity Crew, protecting it from being "Iced" by West coast rappers, oxidative reangents, and such. This will in turn promote a greater half-life and greater bioavailability. (And yes, the things that are popping into your head as you're reading this are exactly things that it is currently being experimented with).


Some things I'm specifically trying to figure out (I appologize if I start throwing out jargon, but I work in the polymer industry):
-Is the 'active' being PEGylated being used as a filler (we often like to stick sh*t between the chains), or does it actually being linked into the polymer chain?
-I've found info on cross-linkers, but I haven't seen any cure accelerators (catalysts). Will this reaction cure up without the help of a CA?
-PEG is also a potent laxative.
Click to expand...



god i can see this turning into "methyl" confusion all over again.

the PEGylation that is of significance here has to do with derivitization of protein hormones such as HGH. there is some research that shows it extends the half-life of HGH while retaining activity. there is also a PEGylated MGF out there, however i have not read research verifying that it also extends half-life and maintains activity
 
There are supposedly PEGylated resveratrol products being marketed under the same ticket (extended half-life, bioavailability).

I was familiar with it's use with MGF, but after seeing resv. being used, it got me a little more interested.
 
riseboi said:
Great thread. You may have already come across it but there are some people who have been dosing their resveratrol with low dose miralax (PEG 3350) to help dissolve the res, increase the surface area, and in turn increase the absorption of it. Forgive me if my wording is incorrect. Do you have any thoughts on this?
Click to expand...

you all are confused

taking a supplement with a polyethylene glycol is not pegylation.

pegylation is the chemical addition of PEG to a chemical via a covalent bond.

what you are talking about is taking a supplement along with a liquid PEG in order to increase dispersion and absorption

p.s. polyethylene glycol is a POLYMER not a monomer. ethylene oxide is the MONOMER from which PEG is made. Everything gets f-ed up when people play chemist


this thread is painful to me
 
thesinner said:
actually, resveratrol has ueber fantastic absorption. It just gets destroyed like a Klansman waltzing through South Central at 1am. I'm wondering, though, if you're taking with Miralax, if that would kill absorption because your taking with a very strong laxative. I'm scared this might make "Resvera-turds".

I would think PEGylation would DECREASE surface area since it's now being stuck inside a chain as opposed to being a fine powder.
Click to expand...

it would not be stuck inside the chain. the PEG would simply act as a high molecular weight solvent
 
thesinner said:
Thanks, that's what I was wondering.

And you're right about PEG not being a monomer, that was a typo. I will fix my post.
Click to expand...


another thing to consider is that it is not as simple as just adding PEG groups to a peptide and "voila" you have an active and protracted hormone derivative

if the PEG groups go in the wrong places then they will block important receptor binding sites on the hormone and it will be biologically useless.

successfully pegylating a peptide would probably involve alot of trial and error. that is, doing the reactions under many different conditions and stoichiometric ratios of PEG to peptide. then you would test each reaction product for binding affinity (or just in-vivo activity) and for resistance to degradation by endogenous proteases

so i would be wary of any pegylated hormone product that does not have research substantiating its efficacy
 
You must log in or register to reply here.
Back
Top