Test, dihydrotest, tren, and zeranol alter synthesis of cortisol

LakeMountD

LakeMountD

Doctor Science
Testosterone, dihydrotestosterone, trenbolone acetate, and zeranol alter the synthesis of cortisol in bovine adrenocortical cells

W. K. Isaacson, S. J. Jones and R. J. Krueger
Department of Animal Science, University of Nebraska, Lincoln 68583-0908.

The objective of this study was to examine the effect of anabolic steroids (testosterone, T; dihydrotestosterone, D; trenbolone acetate, B; and zeranol, Z) on cortisol synthesis by cultured bovine adrenocortical cells. Adrenal glands were obtained from slaughter-aged steers (n = 4). Cortical cells were isolated and their steroidogenic capacity was examined. They were plated in multiwell culture plates. At confluence, cells were treated with T, D, B, or Z at 0, 10, 50, 125, or 500 ng/mL (eight wells per treatment). Twenty-four hours after treatment, one-half of each treatment concentration was stimulated with 10(-9) M ACTH. After 8 h of incubation, cortisol concentration in the media was measured using RIA. Cortical cells were removed from the plates using 1 mM EDTA and analyzed for DNA content. Data were subjected to rank transformation and analyzed by randomized complete block design. Adrenocorticotropic hormone stimulated (P < .01) the release of cortisol by more than threefold. Cortisol synthesis was lower (P < .05) in the presence of T, D, and B. Testosterone caused a greater (P < .05) suppression in cortisol production at 50 and 125 ng/mL than did D. The suppression of cortisol synthesis did not differ between B and T or between B and D. Cortisol synthesis was lowered (P < .05) by the presence of T, D, and B in both ACTH-stimulated and nonstimulated cells but was only suppressed in ACTH-stimulated cells of Z-treated cells.
 

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Could you explain the purpose of the study, and what a bodybuilder should get from it?

That was a little above me. Thanks in advance.
 
mywetnightmares said:
What's Zeranol?
Click to expand...
a type of "fake" estrogen

LakeMount did they give any reasons for the differnces in the cortisol supresion between test base and the tren?
 
Skye said:
a type of "fake" estrogen

LakeMount did they give any reasons for the differnces in the cortisol supresion between test base and the tren?
Click to expand...

No couldn't find any differences. It did state this, however, which is interesting:

"Testosterone decreased cortisol synthesis by 24% at
a concentration of 10 ng/mL to the adrenal cells
(Figure 2). In the intact male, testosterone is secreted
in a pulsatile pattern that ranges from 5 to 15 ng/mL
in concentration. The decrease in cortisol production
ranged from 24 to 30%; the majority of the response
occurred at physiological concentrations of testosterone.
Bulls, which have a higher circulating concentration
of testosterone than either steers or heifers,
exhibited 30 to 50% lower concentrations of cortisol
and produced the leanest, most heavily muscled
carcasses"

"Trenbolone acetate, a synthetic androgen,
decreased cortisol production by 20 to 30% in nonstimulated and ACTH-stimulated cells (Figure 4).
This response was observed with the addition of 10 ng/
mL to the cultured cell (38% in ACTH-stimulated
cells and 27% in nonstimulated cells). Implanting
lambs with TBA reduced plasma glucocorticoids and
increased lean muscle mass (Sulieman et al., 1981;
Sillence et al., 1987). The use of TBA implants in
steers and heifers increased the rate of gain by up to
25% while increasing the lean muscle mass and
nitrogen retention but decreasing plasma cortisol concentrations and fat deposition"
 
I am going to have to read that in full, that is interesting, and not necessarily what I would have thought
at all
Thanks for the post LMD
 
I thought I had been told AAS increases cortisol levels and by the time you stop your cycle cortisol is peaking. guess the cortisol increases due to pct not the AAS. great article.
 
wastedwhiteboy2 said:
I thought I had been told anabolic steroids increases cortisol levels and by the time you stop your cycle cortisol is peaking. guess the cortisol increases due to post cycle therapy not the AAS. great article.
Click to expand...

Yeah I found it extremely interesting as well. However, these are only on some compounds. Non androgen binding drugs may be complete opposite!
 
Well it may be that cortisol rebounds during PCT due to the sudden drop in test and whatever AAS you were running. Test, DHEA and likely all the flavors of androstene are probably antagonists to cort production.
 
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