Supa Freek 420
E-BOLLER
Saw this on bb.com and testosterone.net. Couldn't find anything else on it except for on the Biotest site though. I for one am still skeptical until I hear word that it appears in some peer-reviewed journals. Just thought I'd pass this on.
New Study Proves
MYOSTAT™ (CSP-3)
Increases Hypertrophy 50% in Humans!
For Immediate Release – 12.19.02
A previous study revealed that CSP-3 (an array of sulfated polysaccharides extracted from Cystoseira canariensis) was able to bind the myostatin protein in vitro. Given that myostatin is a negative regulator of muscle growth, if the same myostatin binding effect occurs in vivo (in the body), it was theorized that CSP-3 could increase muscle-protein synthesis and muscle growth.
A more recent placebo controlled, double-blind study was conducted at the Olympic Wrestling Training Center (Zakir Ramazanov, PhD, Musa Abidov, MD, Miguel Jimenez del Rio, PhD. Russia 2002) to examine the effects of orally administered CSP-3 on muscle-protein synthetic rate and lean mass in competitive, elite athletes.
Results
After 60 days of supplementation, subjects in the CSP-3 group (n=9) gained three times more lean mass (13.67 lb versus 4.4 lb) than subjects in the placebo group (n=9). This threefold greater gain in fat-free mass was statistically significant and was supported by changes in muscle-protein synthetic rate. Specifically, CSP-3 stimulated muscle-protein synthesis 50% more than the placebo. These data indicate that CSP-3 has a significant pharmacological effect on skeletal muscle.
Study Using Olympic-Level Athletes
Eighteen (n=18) competitive, Olympic wrestlers were randomly divided into two groups. Thirty days prior to beginning the clinical study, subjects underwent a period of diet counseling and surveillance. Additionally, subjects were asked to maintain their physical-training program throughout the trial. There was no significant difference in the body mass index of subjects in the two groups. Dietary intake (total calories and macronutrient profiles) did not change during the course of the study.
Twice daily, subjects were required to consume either 500 mg of CSP-3 or a placebo. Subjects had not consumed any dietary supplements or medications for more than four months prior to the study. At baseline and after 60 days of CSP-3 or placebo administration, body composition and mixed-muscle protein synthesis rates were evaluated. Baseline protein synthesis rate assessments were made 24 hours before the clinical study began.
On days 0 and 60 of the study, samples of muscle tissue were taken for evaluation of protein synthesis. Food-record analysis and body-mass index were also included at the laboratory intervention times. Body weight was measured with a standard beam scale and the percentage of body fat was estimated from body density derived from underwater weighing.
Various sterile solutions of a leucine isotope (C13L) were prepared and infused overnight to measure the fractional rate of incorporation of C13L into mixed-muscle protein at baseline and at the end of the 60th day of the supplementation period. The rate of muscle-protein synthesis was measured in serial, muscle-biopsy samples during a continuous infusion of C13L in both groups at baseline and after 60 days of supplementation.
The biopsies were taken at a depth of 2-3 cm at about one-third of the distance from the upper margin of the patella to the anterior superior iliac spine. After local skin anesthesia, incisions through the skin and the muscle fascia were made (one on each leg) while subjects rested in the supine position. Biopsies from both legs were combined and analyzed for protein synthesis rates. Mixed-muscle proteins in the biopsy sample were separated and hydrolyzed. Muscle was homogenized in 10% trichloroacetic acid, and proteins were hydrolyzed for 24 hours at 110°C. The formation of n-acetyl-n-propyl ester of leucine and muscle C13L enrichment was measured using gas chromatography-isotope ratio mass spectrometry.
Conclusion
Even though the results of this study may seem surprising, critical analysis of the available scientific data indicates that sulfated polysaccharides possess a variety of important pharmacological and physiological activities in vivo. Scientific literature clearly indicates that sulfated polysaccharides are physiologically active compounds that participate in the regulation of various cellular processes, including myostatin-binding properties that might open a new dimension in muscle-growth research.
Sulfated polysaccharides are crucial for vital reactions in the body because of their ability to bind various proteins. Aside from the positive effects on muscle mass we observed in athletes supplemented with sulfated polysaccharides isolated from Cystoseira canariensis, these compounds may also prove to be beneficial in the development of drugs to combat certain muscular-related diseases.
Additional human studies are planned to further determine the hypertrophic effects of CSP-3.
Testosterone Magazine 2002
New Study Proves
MYOSTAT™ (CSP-3)
Increases Hypertrophy 50% in Humans!
For Immediate Release – 12.19.02
A previous study revealed that CSP-3 (an array of sulfated polysaccharides extracted from Cystoseira canariensis) was able to bind the myostatin protein in vitro. Given that myostatin is a negative regulator of muscle growth, if the same myostatin binding effect occurs in vivo (in the body), it was theorized that CSP-3 could increase muscle-protein synthesis and muscle growth.
A more recent placebo controlled, double-blind study was conducted at the Olympic Wrestling Training Center (Zakir Ramazanov, PhD, Musa Abidov, MD, Miguel Jimenez del Rio, PhD. Russia 2002) to examine the effects of orally administered CSP-3 on muscle-protein synthetic rate and lean mass in competitive, elite athletes.
Results
After 60 days of supplementation, subjects in the CSP-3 group (n=9) gained three times more lean mass (13.67 lb versus 4.4 lb) than subjects in the placebo group (n=9). This threefold greater gain in fat-free mass was statistically significant and was supported by changes in muscle-protein synthetic rate. Specifically, CSP-3 stimulated muscle-protein synthesis 50% more than the placebo. These data indicate that CSP-3 has a significant pharmacological effect on skeletal muscle.
Study Using Olympic-Level Athletes
Eighteen (n=18) competitive, Olympic wrestlers were randomly divided into two groups. Thirty days prior to beginning the clinical study, subjects underwent a period of diet counseling and surveillance. Additionally, subjects were asked to maintain their physical-training program throughout the trial. There was no significant difference in the body mass index of subjects in the two groups. Dietary intake (total calories and macronutrient profiles) did not change during the course of the study.
Twice daily, subjects were required to consume either 500 mg of CSP-3 or a placebo. Subjects had not consumed any dietary supplements or medications for more than four months prior to the study. At baseline and after 60 days of CSP-3 or placebo administration, body composition and mixed-muscle protein synthesis rates were evaluated. Baseline protein synthesis rate assessments were made 24 hours before the clinical study began.
On days 0 and 60 of the study, samples of muscle tissue were taken for evaluation of protein synthesis. Food-record analysis and body-mass index were also included at the laboratory intervention times. Body weight was measured with a standard beam scale and the percentage of body fat was estimated from body density derived from underwater weighing.
Various sterile solutions of a leucine isotope (C13L) were prepared and infused overnight to measure the fractional rate of incorporation of C13L into mixed-muscle protein at baseline and at the end of the 60th day of the supplementation period. The rate of muscle-protein synthesis was measured in serial, muscle-biopsy samples during a continuous infusion of C13L in both groups at baseline and after 60 days of supplementation.
The biopsies were taken at a depth of 2-3 cm at about one-third of the distance from the upper margin of the patella to the anterior superior iliac spine. After local skin anesthesia, incisions through the skin and the muscle fascia were made (one on each leg) while subjects rested in the supine position. Biopsies from both legs were combined and analyzed for protein synthesis rates. Mixed-muscle proteins in the biopsy sample were separated and hydrolyzed. Muscle was homogenized in 10% trichloroacetic acid, and proteins were hydrolyzed for 24 hours at 110°C. The formation of n-acetyl-n-propyl ester of leucine and muscle C13L enrichment was measured using gas chromatography-isotope ratio mass spectrometry.
Conclusion
Even though the results of this study may seem surprising, critical analysis of the available scientific data indicates that sulfated polysaccharides possess a variety of important pharmacological and physiological activities in vivo. Scientific literature clearly indicates that sulfated polysaccharides are physiologically active compounds that participate in the regulation of various cellular processes, including myostatin-binding properties that might open a new dimension in muscle-growth research.
Sulfated polysaccharides are crucial for vital reactions in the body because of their ability to bind various proteins. Aside from the positive effects on muscle mass we observed in athletes supplemented with sulfated polysaccharides isolated from Cystoseira canariensis, these compounds may also prove to be beneficial in the development of drugs to combat certain muscular-related diseases.
Additional human studies are planned to further determine the hypertrophic effects of CSP-3.
Testosterone Magazine 2002
