After trying to reason with my self and seeing how so many people lifes got fuked up by quest machine always screwing up giving in accurate readings. They say the machine is calibrated, but when you are getting readings of <2 on time then <40 the next there is some thing seriously wrong. I have been chasing e2 for 2 years and I honestly got worse as I started to rely on this test. The machine would say I was low then in reality I was really high estrodial because I known the differnce. IMO it is no longer "The Gold standard" in estrodial testing, but only a method to charge the insurance companies huge amounts of money for an invalid test while mean while making 1,000 of guys life miserable and confusing the **** out of most dr's. Dr's are making wrong choices based on this information and this is really effecting the life of their patients. For now I am suggesting patients (as discussed over my colleague get sensitive testing (13-54) vs ultrasensitive <2. I had several dr's call up and complain about the calibration of the machine. I would suggest that untill they get the machine calibrate properly to go back to classic estrodial sensitive tests to avoid any pitfalls in TRT. I know lab corp uses old scale with out any confusion. People that i have seen when changes in TRT were easily seen in the results using this method. I tired of playing guesing games and jeopardizing proper protocol. I even ran rhein urine sample against it and got a more clincal picture with the urine vs ultasensitive test.