Here is a little more detail
Question:
In attempting to make 1000ml of Test Cypionate, the following happened.
When placed into amps, the test started to crystallize out at room temp.
What could the problem be? Here are the steps used to make the liquid
up:
1. Clean and sterilize 1x1000ml round bottom volumetric flask, 2x800ml
beakers, 1x100ml graduated cylinder, 1x1000ml Erlenmeyer filter flask.
2. Heat approximately 800mls of USP oil, 400ml in each beaker at 250
for 45minutes then let cool for 20 minutes.
3. Mix 80mls of BA, and 20mls of BB in the 100ml graduated cylinder
4. Add the 100ml of BA/BB mixture to one of the beakers containing the
oil
5. Begin mixing in 250 grams of test cyp and adding additional oil
6. Pour this mixture into a 1000ml round bottom flask and fill to 1000ml
line with the remaining oil to make a mixture that contains 250mg/ml
cyp, 8%BA, and 2%BB. Stored with a sealed cap...
7. Filter through a 0.45micron filter into the 1000ml Erlenmeyer and
distribute to 10ml amps.
Between steps 6 and 7, some time elapsed(a few days) because the filtering
process takes a while. Meaning that some was filtered and distributed
and then a few days later more was done. One problem that crossed my
mind is that the oil should have been allowed to come all the way back
to room temperature before filling the volumetric flask up to the 1000ml
mark. Thinking that the heated oil expands and that there may not be
enough oil to hold all of that cyp in solution. Meaning that the test
is overdosed and the oil is unable to keep it all in solution so it is
crystallizing out.
One solution that hasn?t been tried yet is to heat all of the cyp back
up to dissolve the crystals, put it all back into the 1000ml volumetric
when cooled and add more oil. Any suggestions or solutions would be
greatly appreciated!!!!
