First homebrew baking process Question

mmorpheuss

mmorpheuss

Registered User
Alright I just made up some 1-test cyp....everything was working fine..

Until I stuck this into my oven at 250 and walked away for 2 minutes.

I come back and the plastic on my needle is melting like the Wicked Witch of the West man. WTF? Obviously I had to abort the operation, as the melted plastic sealed up the hole in the needle.


I'm thinking because I own a convection oven maybe the temperature was too high? I dont want to turn the temperature down too much however, has anyone else had this problem?

Any help is appreciated.
 
If you must bake (personal preference I guess), cut of the top off the plastic with some wire cutters. There will be a little plastic left, but not enough to cover the hole in the pin when it melts.
 
So baking isn't necessary?


I was just following instructions from another thread and wanted to make sure I got it right.
 
mmorpheuss said:
So baking isn't necessary?


I was just following instructions from another thread and wanted to make sure I got it right.
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As long as your are using BA at any percentage above .9%, bacteria cannot grow. However, some prefer to bake. I typically do it just to play it safe. It is best to bake and then cool several times, as it is the heating and cooling that kills bacteria.
 
bow said:
As long as your are using BA at any percentage above .9%, bacteria cannot grow. However, some prefer to bake. I typically do it just to play it safe. It is best to bake and then cool several times, as it is the heating and cooling that kills bacteria.
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Thanks. I cut the excess plastic off and baked it up twice so far no problem :thumbsup:
 
mmorpheuss said:
Thanks. I cut the excess plastic off and baked it up twice so far no problem :thumbsup:
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Glad to hear it worked out.

BTW, for those interested. When I say cooling, the best way to do this is to cool in a frig. It is the rapid heating and then cooling that does the bacteria in.
 
You can also just use a needle to suck the air out a few times, creating a vacuum in the vial. Then there is no risk of pressure buildup. Also this reduces oxidation--evident by the solution turning reddish if you are making tren.
 
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Interesting. Makes sense. Any rules of thumb with regards to the amount of air you draw per vial size prior to heating (ml/ml).
 
Just keep plunging back in and drawing more out until it is obvious there is a vacuum. Using something bigger than 3cc would be a good idea, because then you may only have to pierce the septum once. When you pull up the plunger and it snaps back if you let it go, you've got a pretty decent vacuum.
 
Personally, from what I know about microbiology, I would take the extra step. It should not hurt anything except some of the bacteria the can live in alcohol like some of the members of the Archaea domain but I am overly cautious anyway. IF you could get you hands on an autoclave you would have the best possible setup...
 
Baking to create a sterile product is unnecessary. If your oven was hot enough to melt the plastic on your pin, then it could be hot enough to cook/burn the oil. I've seen it in the past. If your oil becomes foggy after cooling after you have baked it, the the oil is cooked. Pushing your chemicals through a 0.45 micron filter is enough to catch bacteria... and whatever the filter might not catch, the alcohol certainly will. Good luck.
 
MassLabKits said:
Baking to create a sterile product is unnecessary. If your oven was hot enough to melt the plastic on your pin, then it could be hot enough to cook/burn the oil. I've seen it in the past. If your oil becomes foggy after cooling after you have baked it, the the oil is cooked. Pushing your chemicals through a 0.45 micron filter is enough to catch bacteria... and whatever the filter might not catch, the alcohol certainly will. Good luck.
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sorry i disagree...

the filters cannot catch all bacterias, and yes even the .20. even whatman will tell you that, its in their website.
 
kojie said:
sorry i disagree...

the filters cannot catch all bacterias, and yes even the .20. even whatman will tell you that, its in their website.
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Umm, you failed to see this part of my response Kojie:

"... and whatever the filter might not catch, the alcohol certainly will. Good luck."
 
MassLabKits said:
Umm, you failed to see this part of my response Kojie:

"... and whatever the filter might not catch, the alcohol certainly will. Good luck."
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Yes, but then again, it depends on the % of BA used. If enough BA is used, I tend to agree.
 
MassLabKits said:
Umm, you failed to see this part of my response Kojie:

"... and whatever the filter might not catch, the alcohol certainly will. Good luck."
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and again i will disagree not all can be killed by BA, sorry.
 
kojie said:
and again i will disagree not all can be killed by BA, sorry.
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I agree... BA stops the propagation/growth of bacteria but won't necessarily kill all of it. I would play it safe and always bake. What's the big deal with spending an hour or so to bake?? A small price to ensure sterility.

I've baked alot of gear in the past and have never seen the plastic on the needle melt. I always bake at 225-250 deg F for 15 minutes and rapidly cool 3 times. I also use a thermometer to make sure the oven temp is accurate. If the plastic on your needle melted, i believe that the temp must have been way higher than 250 deg.
 
"gram negative bacteria benzyl alcohol"

try searching google for that there are hundreds of articles about it
 
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