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DL-185™ Dileucine Peptide
Research Grade Natural Anabolic Peptide
Backed by Human Clinical Studies
Research Grade Natural Anabolic Peptide
Backed by Human Clinical Studies
This article is to discuss DL-185™ Dileucine Peptide and break it down into simple terms to help make it easy for people to understand – what it is, what it does, and how significant that it is.
DL-185™ is a registered trademark and patented ingredient of Ingenious Ingredients LP, Texas, USA.
Supplements containing DL-185™:
- DL-185™ is available by itself from Competitive Edge Labs (CEL) as MPC-185. (will be available next week)
- DL-185™ is available in combination with another natural anabolic peptide that was discovered in using advanced AI technology, PeptiStrong®, in Serious Nutrition Solutions (SNS) Pepti-Bolic.
DL-185™ Highlights/Supports:
- Lean muscle & strength gains
- Enhances muscle repair & recovery
- Boosts muscle protein synthesis by 159%
- Anabolic & anti-catabolic properties
- 60% greater activation of muscle protein synthesis as compared to leucine
- Dramatically boosts lower body strength, total strength, & lower body reps to failure
- Supported by multiple human clinical research studies
- Weight trainers
- Fitness enthusiasts
- Performance focused athletes
- Recovery focused athletes
- Healthy aging & longevity enthusiasts
- People concerned with losing muscle & strength
- Anyone that wants to increase strength, perform better, and recover better
What is DL-185™?
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DL-185™ is a clinically researched natural anabolic and anti-catabolic peptide composed of two leucine molecules bonded together, creating a unique, patented dipeptide structure.
DL-185™ has been shown in human clinical studies to boost lower body strength, total strength, & lower body reps to failure. It has also been shown to increase muscle protein synthesis better than regular leucine - and not just a ‘little better’ either!
159% Greater Activation of Muscle Protein Synthesis
In a groundbreaking study, subjects who consumed 2,000 mg. of DL-185™, the same dosage found in MPC-185 and Pepti-Bolic, demonstrated a 159% greater activation of muscle protein synthesis compared to baseline and 60% greater than regular leucine.
Anabolic Power that Translates into Real World Strength Gains
Activating muscle protein synthesis is cool, but how does this translate into real world results?
Researchers set out to answer that question in a 10-week resistance training clinical study and the results were incredible! Researchers tested lower body strength, total strength, lower body reps to failure, and total reps to failure. There were three groups tested – one group that took 2,000 mg. of DL-185™, one group that took Leucine, and a third that took a placebo.
The results were outstanding:
- More Total Strength: Subjects taking DL-185™ significantly increased total max strength by 144% as compared to placebo (DL-185™ 154 lbs. vs Placebo 63 lbs.)
- More Lower Body Strength: Subjects significantly increased their lower body max strength by 189% compared to placebo (DL-185™ 133 lbs. vs. Placebo 46 lbs.). So, if legs are your lagging body-part or if you have trouble gaining leg strength, DL-185™ may be just what you need!
- More Reps Than Leucine: Subjects taking DL-185™ significantly increased leg press repetitions to failure by 220% compared to leucine (DL-185™ 16 extra reps vs leucine 5 extra reps).
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Let’s Take a Closer Look at the Research:
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We live in a world where there are so many studies and different levels of studies that sometimes we don’t know what to believe anymore. Luckily, there’s no having to wonder about the validity and significance of the studies on DL-185™.
Study #1:
In a double-blind, randomized, crossover study published in 2021 in the Journal of Applied Physiology, researchers found that DL-185™ is more effective than leucine at stimulating muscle protein synthesis in healthy young males.
- Study: Dileucine Ingestion Is More Effective than Leucine in Stimulating Muscle Protein Turnover in Young Males: A Double Blind Randomized Controlled Trial (Paulussen, Kevin J.M., et al.)
- Study Type: Double-blind, randomized, crossover study.
- Study Objective: To assess the effects of dileucine and leucine ingestion on the modulation of muscle protein synthesis and protein breakdown rates in healthy young adults.
- Participants: 10 healthy, recreationally active males
- Results: Dileucine increased muscle protein synthesis by 159% compared to baseline and 60% more than leucine.
Study #2:
A randomized double-blind placebo controlled study that was originally presented at the 2023 International Society of Sports Nutrition (ISSN) conference was recently published in the prestigious journal PLOS One. This may not mean anything to the average person who is not familiar with these terms, but to anyone that is, they know how significant that this is.
PLOS One is a peer-reviewed open access scientific journal that prioritizes research with strong methodology and high ethical standards, not just groundbreaking findings and all articles undergo a thorough peer review process.
This study validates earlier findings that demonstrated DL-185™ has superior effects compared to leucine for increased muscle protein synthesis (MPS) and adds to the growing evidence showing DL-185’s role in supporting muscle, strength, and performance.
- Study: Dileucine ingestion, but not leucine, increases lower body strength and performance following resistance training: A double-blind, randomized, placebo-controlled trial (Hagle, et. al 24)
- Study Type: Randomized, double-blind placebo control human trial.
- Study Objective: Examine Dileucine’s effects on resistance training adaptations
- Study Duration: 10 weeks
- Study Participants: 32 resistance-trained males
The researchers employed a robust 10 week design that divided the participants into three groups:
- Dileucine group: 2 grams DL-185 dileucine
- Leucine group: 2 grams leucine
- Placebo group: 2 grams resistant starch
The double-blind nature of the study means that neither the participants nor the researchers knew which supplement they were receiving, thereby eliminating any potential bias.
Participant Characteristics:
This study consisted of 32 resistance trained males with the following characteristics:
- Average age: 28 years old (+/- range 6 years)
- Average height: approximately 5’8 to 5’10 (+/- range of 2.9 inches)
- Average weight: 175.7 lbs. (+/- range of 27.6 lbs.)
- Average body mass index (BMI): 25.7 (+/- 3.9 kg/ m²)
- Average body fat percentage: 19% (+/- range if 5.7%)
- Minimum training experience: All participants had at least 1 year of prior resistance training experience. This was verified by the requirement of participants to meet specific strength training markers.
- Participants were required to discontinue all ergogenic nutritional supplements except for multi-vitamins/miners for at least 30 days before and after the study and during the study.
- Participant candidates that reported using any anabolic-androgenic steroids within the prior 12 months were excluded from the study.
Training Regimen:
The training program was rigorously designed to ensure consistency across all three groups.
- 4 training sessions per week
- Split body routine targeting all major muscle groups
- 3-4 sets per exercise
- 6-10 reps per set
- 70%-85% of one-rep maximum intensity range
Key Strength-Related Findings:
The study demonstrated several significant outcomes:
- Leg Press Strength: The dileucine group showed significantly greater increases in leg press one rep max compared to placebo. The difference was dramatic, with a 95% confidence interval of 5.8 to 73.2 kg. improvements.
- Muscular Endurance: The dileucine group showed greater improvements in leg press repetitions to failure compared to the leucine group. The endurance improvements ranged from 0.58 to 20.3 additional reps.
- Total Strength: The dileucine group showed greater increases in total strength (leg press + bench press) compared to placebo. The improvements showed a 95% confidence interval of 6.8 to 75.9 kg.
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The Significance of Outperforming Leucine
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Muscle protein is in a constant process of being continuously broken down and resynthesized. Muscle building takes place when the rate of muscle protein synthesis exceeds the rate of muscle protein breakdown. One specific amino acid - L-Leucine is the most powerful and has long been regarded as an anabolic trigger – turning on muscle protein synthesis.
Muscle protein synthesis (MPS) is the process by which your body uses amino acids to build new muscle protein, essentially meaning it’s how your muscles grow and repair themselves after exercise by creating new muscle tissue from the protein you consume.
Muscle protein synthesis is crucial for muscle growth, recovery, and overall muscle heath. It’s basically the body’s way of repairing and growing muscle tissue, making it a key factor in building muscle mass.
Physical activity, especially resistance training, triggers muscle protein synthesis by creating microscopic tears in muscle fibers that need to be repaired.
Triggering muscle protein synthesis helps to repair and rebuild the muscle fingers that you broke down during exercise. This rebuilding process is what leads to muscle growth (hypertrophy). In order to make this process as efficient as possible, you need to ensure you’re consuming adequate protein (the building blocks of muscles), and it’s essential to stimulate muscle protein synthesis (MPS is a process, not a protein).
Leucine has been shown in clinical research to be a key regulator in the pathways of muscle protein synthesis, playing a crucial role in activating muscle anabolism, and without its presence, this activation doesn’t happen. Therefore, the faster leucine gets absorbed and delivered into the muscles, the faster it can activate muscle protein synthesis and turn on this key pathway of growth and recovery.
This is why leucine is considered the amino acid gold standard as a baseline comparison for how well other ingredients activate muscle protein synthesis, and why when researching supplement ingredients, comparing their muscle protein synthesis activation to leucine is a common baseline for analysis.
Misconceptions & What This Means:
There are a lot of misconceptions about Leucine’s role in muscle protein synthesis and what this all means and about its limitations.
A lot of people, if they read a study where something is being compared to leucine for muscle protein synthesis activation, they mistakenly think that it means to take more leucine or that the ingredient being studied is being compared directly to leucine – that is NOT the case!
Leucine is the gold standard for amino acid muscle protein activation – the study is comparing the amount of activation on muscle protein synthesis – leucine is being used as the baseline comparative standard.
There is a big difference between total leucine intake and the amount of leucine that activates muscle protein synthesis (MPS), and the key difference lies in how your body processes it and the threshold needed to initiate or trigger MPS.
What is leucine’s role in muscle protein synthesis?
Leucine is a branched chain amino acid and essential amino acid that acts as a ‘trigger’ for muscle protein synthesis (MPS).
What does this mean?
When you consume leucine, it gets absorbed into your bloodstream, and it plays a key role in activating the mTOR (mechanistic target of rapamycin) pathway, which is essential for MPS. This activation is what kicks off the muscle repair and muscle growth process.
Wouldn’t it be better to just take more leucine?
No. There is a threshold of leucine needed to activate this pathway effectively (2-3 grams). Once that threshold is reached, taking more leucine doesn’t produce a significantly greater effect on MPS.
This is where a lot of people get confused because they think – well, why not just take more leucine? Because taking more leucine isn’t going to activate any additional muscle protein synthesis – which is why we look for things that are more powerful than leucine for activating MPS.
In Summary:
- Leucine activates muscle protein synthesis (MPS) by triggering the mTOR pathway, but there is a threshold (around 2-3 grams at once) after which additional leucine does not provide more benefit for increasing MPS.
- Total leucine intake over the day matters, but more leucine in one meal does not mean better muscle growth. In simple terms, you can’t rely on eating a lot of protein or taking a scoop of leucine per day to increase MPS.
- So, the difference between activating MPS and just taking more leucine is that after a certain point, your body isn’t going to trigger more muscle growth from leucine alone.
- Leucine Activation of mTOR: As mentioned, leucine activates muscle protein synthesis by stimulating the mTOR pathway. However, there’s a threshold beyond which additional leucine doesn’t provide a significantly greater boost to mTOR activation or MPS. The body has a limit to how much Leucine it can use in one go for this purpose.
- Other Factors at Play: If a different compound or nutrient is more effective at stimulating MPS, it may work through a different mechanism or pathway that complements or augments what leucine does. For example, certain peptides, hormones, or other amino acids might activate the mTOR pathway more robustly or could promote MPS through different signaling mechanisms that leucine alone doesn’t trigger as effectively.
- Leucine Saturation: Once leucine reaches a certain threshold and mTOR is maximally activated, additional leucine doesn’t have a greater effect. But another compound that stimulates MPS more effectively might continue to boost the rate of muscle growth or enhance recovery, even if you’re already getting the maximal effect from leucine.
- Synergy with Other Nutrients: Many other compounds or amino acids might work in synergy with leucine, improving MPS through complementary mechanisms. For example, there are some ingredients/nutrients that can also enhance MPS in ways that leucine alone can’t replicate.
Remember – Muscle Protein Synthesis is a PROCESS!
So, when something outperforms the gold standard for comparative analysis by 60%, that’s a big deal!
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If you are interested in boosting your muscle protein synthesis by 159% and increasing lower body strength, total strength, reps and accelerating muscle repair and recovery, DL-185™ is available in:
MPC-185 – link will be added upon release next week
Pepti-Bolic - https://seriousnutritionsolutions.com/product/pepti-bolic/