Lightweight1
Active member
Ok so i had my igf-1 mixed with AA, and my BA water all,in my car and it froze. Did I ruin all of it or will it be ok if I thaw it out and use it ?
Accidentally froze my gear....
- Thread starter Lightweight1
- Start date
Newtonselite
Member
Cant see a problem with it m8, Just be careful reheating because if its still frozen and you do it to fast obviously the glass will crack.
- Either boil it or bake it!
On a side note im sure IGF-1 is recommended to be stored in the freezer? (Could be wrong though on that)
Id be more worried about heat then cold check out wot happened to my test (On the right) from being in the hot car when i moved locations
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Gator 87
Member
If the water holding a peptide actually freezes solid, it is ruined.
The crystal lattice structure that forms when water freezes (ice) will tear apart a peptide chain (since they are long, delicate chains of amino acids).
But the straight bac water is fine, if it didn't contain any peptides.
The crystal lattice structure that forms when water freezes (ice) will tear apart a peptide chain (since they are long, delicate chains of amino acids).
But the straight bac water is fine, if it didn't contain any peptides.
Lightweight1
Active member
Ok but I saw this info stating it was ok at -4 f :
Storage of LR3 IGF-1
*Study conducted by Gropep
The stability of a liquid solution of LR3IGF-I was monitored for a period of two years at storage conditions of -20 C, +4 C, +22 C, and +37 C. The final concentration of LR3IGF-I was in acetic acid. At various time points, samples were taken and compared to a lyophilized control (stored at 4 C). Listed below are the stability results for each respective storage condition.
Storage Condition: -20 C (-4 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years
Storage Condition: +4 C (39.2 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years
Storage Condition: +22 C (71.6 F)
Biological Potency No Change up to 2 years
Immunological Activity No Change up to 2 years
Mobility of Protein No Change up to 2 years
Elution Profile by reversed phased HPLC No Change up to 2 years
Storage Condition: +37 C (98.6 F)
Biological Potency No Change up to 1 year
Immunological Activity No Change up to 1 year
Mobility of Protein No Change up to 1 year
Elution Profile by reversed phased HPLC No Change up to 1 year
In conclusion, there is no significant difference in the potency of LR3IGF-I associated with the storage of the liquid formulation when stored at this range of temperatures. There is no evidence for loss of biological activity at any of the tested temperatures when stored as a liquid product. As you can see IGF can be quite stable for even a year at room temperature, but if you want to keep it around for a while then stick it into the fridge. So in my opinion the best way to store LR3 that is suspended in BA (benzoic acid) is in the freezer. The BA won’t allow it to freeze. And if you have it suspended in AA (acetic acid), store it in the fridge.
Lightweight1
Active member
It was already reconstituted in AA when it froze since then i have thawed it.. i hope its not completely ruined. :grumpy:
BlondBombshel
New member
buy more!
Gator 87
Member
Yeah, but the key here is that it did not actually freeze, because of the BA (and because the temp was contolled).
However, the OP's did freeze solid.
The temp does not matter, but the change to a solid state is what destroys the peptide.
Lightweight1
Active member
Well i learned a 120 dollar lesson the hard way. I think when my new batch arrives I'll just reconstitute in AA this time and leave it at room temp and call it a day.
thanks for everyone's help
2res4
Member
I had a bottle in the fridge that appeared to be freezing. I took it out and let it sit at room temp for 48 hours. It wasn't freezing, but actually started to crystalize. The company stated it was from the way it was lypholized or lack of something. It worked fine and they sent me a bunch of replacements. I transfered the liquid into another vial. I then broke the crystal apart with a syringe and it was solid and jagged. Any ever seen that?
Lightweight1
Active member
No i have not seen that. Mine totally dissolved when i added the AA into it. I just left mine in the car and the temp dropped here in IL and it all froze. Ill just be ordering more now.
Ive noticed online that there is a big difference in price depending on the website. Have you used any of the cheaper stuff and if so how does it compare to the higher priced stuff. Ive been spending around 120 for 1ml is that too much?
2res4
Member
Hey bro, I would continue to use the vial that froze but keep it refridgerated. If you do some research people recomend pre loading syringes and freezing them until needed and I have read some threads from very respected people on this topic who do this all the time. The reason is to ensure it last longer. So it seems to me freezing does not ruin lr3. I know people are freezing follistatin as well because of the half life. You should be all set.