Dietary Protein Distribution Positively
Influences 24-h Muscle Protein Synthesis in
Healthy Adults1
–3
Madonna M. Mamerow,4 Joni A. Mettler,4 Kirk L. English,4 Shanon L. Casperson,6 Emily Arentson-Lantz,4 Melinda Sheffield-Moore,6 Donald K. Layman,7 and Douglas Paddon-Jones4,5*
4Division of Rehabilitation Sciences, 5Department of Nutrition and Metabolism, and 6Department of Internal Medicine, The University of Texas Medical Branch, Galveston, TX; and 7Department of Food Science and Human Nutrition, University of Illinois at Urbana- Champaign, Champaign, IL
Abstract
The RDA for protein describes the quantity that should be consumed daily to meet population needs and to prevent deficiency. Protein consumption in many countries exceeds the RDA; however, intake is often skewed toward the evening meal, whereas breakfast is typically carbohydrate rich and low in protein. We examined the effects of protein distribution on 24-h skeletal muscle protein synthesis in healthy adult men and women (n = 8; age: 36.9 6 3.1 y; BMI: 25.7 6 0.8 kg/ m2). By using a 7-d crossover feeding design with a 30-d washout period, we measured changes in muscle protein synthesis in response to isoenergetic and isonitrogenous diets with protein at breakfast, lunch, and dinner distributed evenly (EVEN; 31.5 6 1.3, 29.9 6 1.6, and 32.7 6 1.6 g protein, respectively) or skewed (SKEW; 10.7 6 0.8, 16.0 6 0.5, and 63.4 6 3.7 g protein, respectively). Over 24-h periods on days 1 and 7, venous blood samples and vastus lateralis muscle biopsy samples were obtained during primed (2.0 mmol/kg) constant infusion [0.06 mmol/(kgmin)] of L-[ring-13C6] phenylalanine. The 24-h mixed muscle protein fractional synthesis rate was 25% higher in the EVEN (0.075 6 0.006%/h) vs. the SKEW (0.056 6 0.006%/h) protein distribution groups (P = 0.003). This pattern was maintained after 7 d of habituation to each diet (EVEN vs. SKEW: 0.077 6 0.006 vs. 0.056 6 0.006%/h; P = 0.001). The consumption of a moderate amount of protein at each meal stimulated 24-h muscle protein synthesis more effectively than skewing protein intake toward the evening meal. J. Nutr. doi: 10.3945/jn.113.185280.
