No. In the amounts they're talking, there would be no risk for an increase in acetylcholine in the body. Because Prime and aminos like Carnitine, etc increase acetylcholine more or less naturally, it's safe. It's involved in so many critical processes in the body in relation to muscle contraction and other interactions between all 3 types of muscle and the nervous system. Were your body to produce more acetylcholine than it needed or wanted, and started to see it as a possible danger, your body would produce more acetylcholinesterase, which is the enzyme that slows down the activity of acetylcholine by attaching itself to the receptor sites.
To give you an idea of what I mean, Nerve agents that the military and terrorists use on people, work by stopping the production of acetylcholinesterase, thereby allowing the acetylcholine to continue to be fired off and causing rapid uncontrolled muscle contractions, seizures, etc.
So in short, nah you're good, bro.