RPM Samples, plus a little teaser for the write-up...

Page 1 of 10 1236 ... Last
  1. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    RPM Samples, plus a little teaser for the write-up...


    We have some ready to go if anyone is interested!!!!!!


    dirkt@bellsouth.net

    Introducing RPM, the supplement world’s first Anabolic-Cognitive Energy System Enhancer (A-CESE), where anabolics meet cognitive enhancement!
    An A-CESE is a product that contains testosterone-like, anti-catabolic, and aromatase-inhibiting properties, while at the same time delivering optimal mind/muscle connection/contraction through unique peripheral vasostimulatory perfusion and increased cognitive psycho-motor control. RPM combines P-SARM Synthase AI- a unique blend of research-grade icariin (a unique phytochemical SARM), L-Arginine, Naringenin, and oligomeric proanthocyanidins (OPC’s), and Methyl-AMP Complex, a powerful blend of methyl-xanthine caffeine and chocamine. This creates a product completely unique to the market and in a class by itself- try a sample and find out for yourself!!!

  2. Board Sponsor
    mmowry's Avatar
    Stats
    5'9"  260 lbs.
    Join Date
    Apr 2004
    Age
    43
    Posts
    2,213
    Rep Power
    1600
    Level
    34
    Lv. Percent
    21.5%
    Achievements Activity ProPosting Pro

    Im VERY EXCITED.This looks like winner fo sho!Great job!!!


    Trust in the LORD with all your heart, And lean not on your own understanding; In all your ways acknowledge Him, And He shall direct your paths . Proverbs 3:5-6
  3. Elite Member
    john123131's Avatar
    Join Date
    Mar 2007
    Posts
    5,792
    Rep Power
    2989
    Level
    50
    Lv. Percent
    9.1%
    Achievements Activity ProPosting ProPosting Authority

    great company, and this looks awesome...Im in
    •   
       

  4. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by john123131 View Post
    great company, and this looks awesome...Im in
    Thanks guys!!!
  5. O.G. Appnut
    Vitruvian's Avatar
    Join Date
    Dec 2005
    Posts
    5,203
    Rep Power
    85509
    Level
    49
    Lv. Percent
    31.7%
    Achievements Activity ProPosting ProPosting Authority

    Guys, that little teaser write-up doesn't even do this product justice.... seriously. Get your samples and see what I mean.

    "New PR's for everyone.... get your new PR's here.... Step right up....."
    Applied Nutriceuticals Representative
    Better Results Through Science
    Success occurs when everything you have is APPLIED.
    Have you heard about N.O. Uptake? ;-)
  6. O.G. Appnut
    Vitruvian's Avatar
    Join Date
    Dec 2005
    Posts
    5,203
    Rep Power
    85509
    Level
    49
    Lv. Percent
    31.7%
    Achievements Activity ProPosting ProPosting Authority

    It may sound like hype, but it's fact.
    Applied Nutriceuticals Representative
    Better Results Through Science
    Success occurs when everything you have is APPLIED.
    Have you heard about N.O. Uptake? ;-)
  7. AI Sports Fitness Expert
    buffhunk29's Avatar
    Join Date
    Sep 2005
    Age
    30
    Posts
    1,497
    Rep Power
    866
    Level
    28
    Lv. Percent
    99.1%
    Achievements Posting Pro

    AWESOME Write up!!


    Everyone get your SAMPLES NOW!!! They wont last long be the FIRST to get a chance to try out the FUTRUE OF SUPPLEMENTS!!!!
  8. New Member
    addijhaq's Avatar
    Join Date
    Nov 2005
    Posts
    111
    Rep Power
    164
    Level
    9
    Lv. Percent
    25.12%

    finally, something for the scholarly gym rat
  9. Board Sponsor
    poison's Avatar
    Stats
    6'0"  190 lbs.
    Join Date
    Oct 2004
    Age
    40
    Posts
    5,904
    Rep Power
    37731
    Level
    51
    Lv. Percent
    44.23%
    Achievements Activity ProPosting ProPosting Authority

    I'm waiting by the mailbox.
  10. NutraPlanet NinjaMonkey Rep
    Steveoph's Avatar
    Stats
    6'0"   lbs.
    Join Date
    Jan 2007
    Posts
    12,524
    Rep Power
    41245
    Level
    67
    Lv. Percent
    41.2%
    Achievements Activity AuthorityActivity ProPosting ProPosting AuthorityPosting Veteran

    That writeup is quite tempting. Waiting for my sample!
  11. Diamond Member
    John Smeton's Avatar
    Stats
    6'2"  225 lbs.
    Join Date
    Sep 2004
    Age
    33
    Posts
    10,014
    Rep Power
    33558
    Level
    62
    Lv. Percent
    81.82%
    Achievements Activity AuthorityActivity ProPosting ProPosting AuthorityPosting Veteran

    Sent. Interesting. I think everyone in the bodybuilding community are waiting for the SARMS to be developed. This is the Sarm in the beginning stages correct. So no side effects and all muscle correct?should one with male pattern baldness be concerned about this product because I know the true SARMS that are predicted be done in the next five years have no side effects , only muscle growth.
  12. New Member
    nvr2loud's Avatar
    Join Date
    Sep 2005
    Posts
    301
    Rep Power
    261
    Level
    14
    Lv. Percent
    54.95%

    Just sent you an e-mail. Can't wait to try this out!
  13. Elite Member
    djbombsquad's Avatar
    Stats
    5'5"  150 lbs.
    Join Date
    Dec 2006
    Posts
    6,897
    Rep Power
    365462
    Level
    58
    Lv. Percent
    2.61%
    Achievements Activity ProActivity AuthorityPosting ProPosting Authority

    Is this the real deal for sarm?
  14. Diamond Member
    John Smeton's Avatar
    Stats
    6'2"  225 lbs.
    Join Date
    Sep 2004
    Age
    33
    Posts
    10,014
    Rep Power
    33558
    Level
    62
    Lv. Percent
    81.82%
    Achievements Activity AuthorityActivity ProPosting ProPosting AuthorityPosting Veteran

    All Sarms are in beginning stages Jim.
  15. Elite Member
    djbombsquad's Avatar
    Stats
    5'5"  150 lbs.
    Join Date
    Dec 2006
    Posts
    6,897
    Rep Power
    365462
    Level
    58
    Lv. Percent
    2.61%
    Achievements Activity ProActivity AuthorityPosting ProPosting Authority

    Is this supplement the real deal for Sarm? I know what sarm is but I am wondering about this supply in question.
  16. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by djbombsquad View Post
    Is this supplement the real deal for Sarm? I know what sarm is but I am wondering about this supply in question.
    Icariin is a derivative of the plant epimedium sagittarius (horny goat weed)- RPM uses a high percentage extract of Icariin (50%) in very appreciable doses. 95% of the products during the "Horny Goat Weed" supplement craze were a 5-20% icariin extract, and not used in correct dosages- this steered a great deal of bodybuilders away from this supplement. Now we are going to bring Icariin back, in the form of the highly effective compound that it is!!!!! When I started research high-dose Icariin, I was honestly really surprised that no one did this sooner- too many benefits/applications to bodybuilding to ignore........

    The prerequisites of a SARM (selective androgen reuptake modulator) are the ability of a compound to: "stimulate increases in strength and fat-free mass through testosterone mimetic properties, supporting bone growth, and maintaining and restoring sexual function and general "maleness", while being orally bioavaliable and not effecting blood pressure or blood lipids." Icariin fits every one of these prerequesites, here are some studies:

    Asian J Androl. 2006 Sep;8(5):601-5. Epub 2006 Jun 5.Click here to read Links
    The testosterone mimetic properties of icariin.

    * Zhang ZB,
    * Yang QT.

    Department of Urology, Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, China. zbzhang@stu.edu.cn

    AIM: To evaluate the testosterone mimetic properties of icariin. METHODS: Forty-eight healthy male Sprague-Dawley rats at the age of 15 months were randomly divided into four groups with 12 rats each: the control group (C), the model group (M), the icariin group (ICA) and the testosterone group (T). The reproductive system was damaged by cyclophosphamide (intraperitoneal injection, 20 mg/kg x day) for 5 consecutive days for groups M, ICA and T, at the sixth day, ICA (gastric gavage, 200 mg/kg x day) for the ICA group and sterandryl (subcutaneous injection, 5 mg/rat . day) for the T group for 7 consecutive days, respectively. The levels of serum testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH), serum bone Gla-protein (BGP) and tartrate-resistant acid phosphatase activity in serum (StrACP) were determined. The histological changes of the testis and the penis were observed by microscope with hematoxylin-eosin (HE) staining and terminal deoxynucleotidyl transferase biotin-dUTP-X nick end labeling (TUNEL), respectively. RESULTS: (1) Icariin improved the condition of reproductive organs and increased the circulating levels of testosterone. (2) Icariin treatment also improved the steady-state serum BGP and might have promoted bone formation. At the same time, it decreased the serum levels of StrACP and might have reduced the bone resorption. (3) Icarrin suppressed the extent of apoptosis of penile cavernosal smooth muscle cells. CONCLUSION: Icariin has testosterone mimetic properties and has therapeutic potential in the management of hypoandrogenism.


    Pharmazie. 2005 Dec;60(12):939-42. Links
    Icariin, a flavonoid from the herb Epimedium enhances the osteogenic differentiation of rat primary bone marrow stromal cells.Chen KM, Ge BF, Ma HP, Liu XY, Bai MH, Wang Y.
    Institute of Orthopaedics, Lanzhou General Hospital, Lanzhou, Gansu 730050, PR China. Chkeming@yahoo.com.cn

    The herb Epimedium has long been used in Traditional Chinese Medicine to treat bone fracture and prevent osteoporosis. Researchers believe that the flavonoids contained in the herb are the effective component for this activity. However, no single flavonoid has been studied for its effect on bone-related cells. In the present study, icariin, one of the major flavonoids of the herb, supplemented the primary culture medium of rat bone marrow stromal cells (rMSCs) at 0.1 microM , 1 microM and 10 microM respectively. It was found that icariin stimulated the proliferation of rMSCs and increased the number of CFU-F stained positive for alkaline phosphatase in a dose-dependent manner. Icariin also dose-dependently increased the alkaline phosphatase activity, osteoalcin secretion and calcium deposition level of rMSCs during osteogenic induction. The addition of 10 microM icariin caused four times more mineralized bone nodules to be formed by rMSCs than in the control. The results demonstrated that icariin should be an effective component for bone-strengthening activity, and one of the mechanisms is to stimulate the proliferation and enhance the osteogenic differentiation of MSCs.

    Pharmacol Biochem Behav. 2005 Dec;82(4):686-94. Epub 2005 Dec 27. Links
    Antidepressant-like effect of icariin and its possible mechanism in mice.Pan Y, Kong L, Xia X, Zhang W, Xia Z, Jiang F.
    State Key Laboratory of Pharmaceutical Biotechnology, Immunobiological Laboratory, Institute of Functional Biomolecule, Nanjing University, PR China.

    The behavioral, neurochemical and neuroendocrine effects of icariin isolated from Epimedium brevicornum were investigated in behavioral despair models of KunMing strain of male mice. Icariin was found to significantly shorten immobility time in the forced swimming test (FST) after orally administration for 21 consecutive days. Icarrin also produced a marked reduction in immobility time in the tail suspension test (TST) when administered for at least 7 consecutive days. The preferable antidepressant action by icariin was obtained at 17.5 and 35 mg/kg in the present study. Moreover, it was observed that the stress of FST exposure induced increases in brain monoamine oxidase (MAO) A and B activities, serum corticotropin-releasing factor (CRF) levels, as well as decreases in brain monoamine neurotransmitter levels. Treatment of icariin for 21 consecutive days mainly reversed the above effects in the mouse FST. These results suggested that icarrin possessed potent antidepressant-like properties that were mediated via neurochemical and neuroendocrine systems.

    Asian J Androl. 2005 Dec;7(4):381-8. Links
    Effects of icariin on erectile function and expression of nitric oxide synthase isoforms in castrated rats.Liu WJ, Xin ZC, Xin H, Yuan YM, Tian L, Guo YL.
    Andrology Center of Peking University First Hospital, Beijing 100009, China.

    AIM: To investigate the effect of icariin on erectile function and the expression of nitric oxide synthase (NOS) isoforms in castrated rats. METHODS: Thirty-two adult male Wistar rats were randomly divided into one sham-operated group (A) and three castrated groups (B, C and D). One week after surgery, rats were treated with normal saline (groups A and cool.gif or oral icariin (1 mg/[kg.day] for group C and 5 mg/[kg.day] for group D) for 4 weeks. One week after treatment, the erectile function of the rats was assessed by measuring intracavernosal pressure (ICP) during electrostimulation of the cavernosal nerve. The serum testosterone (ST) levels, the percent of smooth muscle (PSM) in trabecular tissue, and the expression of mRNA and proteins of neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS) and phosphodiesterase V (PDE5) in corpus cavernosum (CC) were also evaluated. RESULTS: ICP, PSM, ST and the expression of nNOS, iNOS, eNOS and PDE5 were significantly decreased in group B compared with those in group A (P 0.01). However, ICP, PSM and the expression of nNOS and iNOS were increased in groups C and D compared with those in group B (P 0.05). Changes in ST and the expression of eNOS and PDE5 were not significant (P 0.05) in groups C and D compared with those in group B. CONCLUSION: Oral treatment with icariin ( 98.6 % purity) for 4 weeks potentially improves erectile function. This effect is correlated with an increase in PSM and the expression of certain NOS in the CC of castrated rats. These results suggest that icariin may have a therapeutic effect on erectile dysfunction.

    Wei Sheng Yan Jiu. 2005 Mar;34(2):191-3. Links
    [Effects of Icariin on ovariectomized osteoporotic rats][Article in Chinese]
    Bao JR, Yang JW, Li SF, Zhao W, Zhang Q, Yan Y.
    Life Science College, Northeast Agricultural University, Harbin 150030, China.

    OBJECTIVE: To observe the effects of Icariin on ovariectomized osteoporotic rats. METHODS: Female Wistar rats were ovariectomized and administered different dosage of Icariin and 17beta-estradiol for eight weeks. Bone mineral density (BMD), indexes of biomechanics and bone metabolism-associated biochemical markers were measured. RESULTS: Icariin increased the BMD, maximum load and flexural rigidity in the osteoporotic rats. The activities of serum tartrate-resistant acid phosphatase (TRACP) and bone alkaline phosphatase (BALP) were decreased in the Icraiin-fed ovariectomized rats. CONCLUSION: Icariin 225mg/kg per day could increase the BMD and improve indexes of bone biomechanics in ovariectomized osteoporotic rats. It was effective in preventing bone loss induced by ovariectomy.


    Icariin fits all of the criteria for a SARM, except it is in a very well-researched phytochemical extract, not an undeveloped potential pharmaceutical drug- hence the title Phytochemical SARM (P-SARM)
  17. Registered User
    Zombie's Avatar
    Stats
    5'8"  212 lbs.
    Join Date
    May 2006
    Age
    31
    Posts
    5,584
    Rep Power
    2919
    Level
    49
    Lv. Percent
    41.66%
    Achievements Activity ProPosting ProPosting Authority

    subscribed looking forward to the reviews. I wish i could order a sample but i dont want to take the oportunity of someone to test it out and give a good review. since im doing some testing of my own with some supps
  18. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    I included these two because Icariin is a strong PDE5 inhibitor, just like Viagra and Cialis

    Clin Endocrinol (Oxf). 2004 Sep;61(3):382-6. Links
    Type V phosphodiesterase inhibitor treatments for erectile dysfunction increase testosterone levels.
    Carosa E,
    Martini P,
    Brandetti F,
    Di Stasi SM,
    Lombardo F,
    Lenzi A,
    Jannini EA.
    Department of Experimental Medicine, University of L'Aquila, L'Aquila, Italy.
    OBJECTIVE: Lack of sexual activity due to erectile dysfunction (ED) decreases testosterone (T) levels through a central effect on the hypothalamic-pituitary axis. In this paper we studied the effect of different type V phosphodiesterase (PDE5) inhibitor treatments for ED on the reversibility of this endocrine pattern. DESIGN: Open-label, retrospective study. PATIENTS: Seventy-four consecutive patients were treated on demand with sildenafil (Sild) (50 mg) and tadalafil (Tad) 20 mg. MEASUREMENTS: The success in sexual intercourse was recorded and total (tT) and free testosterone (fT) levels were studied before and after 3 months of treatment. RESULTS: Basal level of tT and fT were at the bottom of the normal range and LH levels were at the top of the high normal range. After treatments, this endocrine pattern was reversed in both groups. However, the T increase in Sild-treated patients was significantly lower than in those treated with Tad (4.7 +/- 2.7 vs. 5.1 +/- 0.9, P < 0.001). fT levels followed a directly proportional pattern, while the inverse was found when LH production was studied. The intercourse rate reflected this effect: in fact, the Sild group showed a 4.9 +/- 2.9/month full sexual intercourse rate while in the Tad group a significantly higher rate of sexual intercourse was found (6.9 +/- 4.6/month, P = 0.04). However, drug consumption was comparable between the groups (Sild 4.9 +/- 2.9 vs. Tad 4.4 +/- 2.8 pills/month, P = 0.72). CONCLUSIONS: As it is unlikely that the two drugs have a different direct effect on the pituitary-testis axis, this effect is probably due to the higher frequency of full sexual intercourse in the Tad-treated group, because of the drug's longer half-life.
  19. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    J Sex Med. 2006 Jul;3(4):716-22. Links
    Testosterone:estradiol ratio changes associated with long-term tadalafil administration: a pilot study.
    Greco EA,
    Pili M,
    Bruzziches R,
    Corona G,
    Spera G,
    Aversa A.
    Internal Medicine, Department of Medical Pathophysiology, University of Roma La Sapienza, Rome, Italy.
    INTRODUCTION: It has been reported that lack of sexual activity due to erectile dysfunction (ED) may be associated with testosterone (T) decline. AIM: To investigate whether the known changes in sex hormones associated with resumption of sexual activity are sustained in the long term. MAIN OUTCOME MEASURES: Primary endpoints were variations from baseline of steroid hormones: total T, free T (f T), and estradiol (E). Secondary endpoints were variations of erectile function domain scores at International Index of Erectile Function-5 (IIEF-5). METHODS: In an open-label fashion, 20 patients (mean age 54.8 +/- 8.4 years) received tadalafil 10-20 mg on demand for 12 months. Exclusion criteria were those reported for phosphodiesterase inhibitors, including hypogonadism and hyperprolactinemia. RESULTS: Tadalafil assumption was safe and well tolerated (overall adverse effects in 15% of patients) and none discontinued medication. A significant decrease in E levels occurred at the end of the study (from 19.9 +/- 9.6 to 16.6 +/- 8.1 ng/dL, P = 0.042 vs. baseline), with parallel increase in the T:E ratio (26.3 +/- 15.3 to 32.6 +/- 17.7, P = 0.05), whereas no changes in T and f T serum levels were observed, respectively (411.4 +/- 131.4 to 434.2 +/- 177.1 ng/dL and 47.7 +/- 15.3 to 49.9 +/- 19.1 pmol/L, not significant). Interestingly, nonparametric subgroup analysis for related samples revealed that E decrease was detectable only in lean (N = 14) but not in obese (N = 6, body mass index > 27.5 kg/m2) subjects (17.8 +/- 10.1 vs. 13.5 +/- 6.8, P < 0.05). A net increase in IIEF-5 scores was observed at the endpoint (13.7 +/- 5.9 vs. 25.7 +/- 2.9, P < 0.0001). CONCLUSIONS: Sustained improvement in sexual function after 12 months of tadalafil administration is associated with increased T:E ratio mainly related to reduction of E levels. We hypothesize that androgen-estrogen cross-talk and possible inhibition of aromatase activity during chronic exposure to tadalafil might have a role in the regulation of erectile function.
  20. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by Zombie View Post
    subscribed looking forward to the reviews. I wish i could order a sample but i dont want to take the oportunity of someone to test it out and give a good review. since im doing some testing of my own with some supps
    I will save some out for you if you really want to try it...
  21. Advanced Member
    brass monkey's Avatar
    Join Date
    Dec 2006
    Posts
    881
    Rep Power
    536
    Level
    23
    Lv. Percent
    17.56%

    Nice offer E-mail sent I had great results with some of your other products. Thanks
  22. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by brass monkey View Post
    Nice offer E-mail sent I had great results with some of your other products. Thanks
    Yeah- I enjoyed your log
  23. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    Bump for the morning crew......
  24. Elite Member
    john123131's Avatar
    Join Date
    Mar 2007
    Posts
    5,792
    Rep Power
    2989
    Level
    50
    Lv. Percent
    9.1%
    Achievements Activity ProPosting ProPosting Authority

    morning guys.....pumped for that sample!
  25. O.G. Appnut
    Vitruvian's Avatar
    Join Date
    Dec 2005
    Posts
    5,203
    Rep Power
    85509
    Level
    49
    Lv. Percent
    31.7%
    Achievements Activity ProPosting ProPosting Authority

    I'm not typical morning crew, but hooooooooooooowdy!
    Applied Nutriceuticals Representative
    Better Results Through Science
    Success occurs when everything you have is APPLIED.
    Have you heard about N.O. Uptake? ;-)
  26. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by Lanbane View Post
    I'm not typical morning crew, but hooooooooooooowdy!
    Hey bud!!!
  27. O.G. Appnut
    Vitruvian's Avatar
    Join Date
    Dec 2005
    Posts
    5,203
    Rep Power
    85509
    Level
    49
    Lv. Percent
    31.7%
    Achievements Activity ProPosting ProPosting Authority

    Hey D,

    I RPM'ed this morning.... 4 caps.
    Did bodyweight dips as my first tricep exercise. Set of 20... felt good.... so I did a set of 30..... felt good..... then a set of 50 which felt awesome, before moving on to skullcrushers, 3 sets of 10 with 100 lbs.

    Damn good numbers for me!!! Me lovey RPM long time.
    Applied Nutriceuticals Representative
    Better Results Through Science
    Success occurs when everything you have is APPLIED.
    Have you heard about N.O. Uptake? ;-)
  28. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    Icariin as a PDE5 Inhibtor


    Urology. 2006 Dec;68(6):1350-4.
    Effects of icariin on phosphodiesterase-5 activity in vitro and cyclic guanosine monophosphate level in cavernous smooth muscle cells.

    * Ning H,
    * Xin ZC,
    * Lin G,
    * Banie L,
    * Lue TF,
    * Lin CS.

    Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, School of Medicine, San Francisco, California 94115, USA.

    OBJECTIVES: To investigate the effect of icariin on the cyclic guanosine monophosphate (cGMP)-hydrolytic activity of phosphodiesterase-5 (PDE5) isoforms and the cGMP levels in cavernous smooth muscle cells treated with sodium nitroprusside (SNP). METHODS: PDE5 isoforms (PDE5A1, A2, and A3) were isolated from sf9 insect cells infected with baculoviruses carrying PDE5 isoform cDNA. Icariin was isolated from Epimedii herba. Varying amounts (10(-6) to 10(-11) M) of icariin or zaprinast were added to reaction mixtures containing PDE5 isoforms and cGMP. The inhibitory effects of icariin and zaprinast were analyzed by GraphPad Software and are expressed as concentration that inhibits 50% (IC50) values. Cavernous smooth muscle cells were isolated from 3-month-old rats, treated with icariin (100 and 200 microM) or zaprinast (200 microM) for 15 minutes, and then with 10 microM SNP for 30, 60, 120, 240, and 360 minutes. The cells were then analyzed for the cGMP concentration using an enzyme immunoassay system. RESULTS: Icariin inhibited PDE5A1, A2, and A3 with an IC50 value of 1.0, 0.75, and 1.1 microM, respectively. The corresponding IC50 values for zaprinast were 0.33, 0.23, and 0.32 microM. Icariin consistently outperformed the control (SNP-only treatment) in maintaining greater cGMP levels, particularly at the greater concentration of 200 microM. In contrast, zaprinast at 200 microM did better than the control only at 60 and 360 minutes. CONCLUSIONS: Icariin was inhibitory to all three PDE5 isoforms with similar IC50 values, which were approximately three times greater than those for zaprinast. Icariin was able to enhance cGMP levels in SNP-treated cavernous smooth muscle cells.


    J Huazhong Univ Sci Technolog Med Sci. 2006;26(4):460-2.
    Effect of icariin on cyclic GMP levels and on the mRNA expression of cGMP-binding cGMP-specific phosphodiesterase (PDE5) in penile cavernosum.


    * Jiang Z,
    * Hu B,
    * Wang J,
    * Tang Q,
    * Tan Y,
    * Xiang J,
    * Liu J.

    Department of Pharmacology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

    To further investigate the mechanisms of action of icariin (ICA), we assessed the effects of ICA on the in vitro formation of cGMP and cAMP in isolated rabbit corpus cavernosum. Isolated segments of rabbit corpus cavernosum were exposed to increasing concentrations of ICA and the dose-dependent accumulation of cGMP and cAMP was determined in the tissues samples by means of 125I radioimmunoassay. Responses of the isolated tissues preparations to ICA were compared with those obtained with the reference compounds sildenafil (Sild). Furthermore, the effects of ICA on the mRNA expression of specific cGMP-binding phosphodiesterase type V (PDE5) in rat penis were also observed. After incubation with ICA for 6 h or 14 h respectively, the levels of PDE5 mRNA were examined by reverse transcriptase polymerase chain reaction (RT-PCR). The results showed that ICA increased cGMP concentrations directly (P < 0.05), but there was no significant effect on cAMP concentrations (P > 0.05). In the presence of sodium nitroprusside (SNP), a stimulatory agent of cGMP, both ICA and Sild increased cGMP concentrations with increasing dose (P < 0.01). Their EC50 was 4.62 (ICA) and 0.42 (Sild) micromol/L respectively. Under the same condition, ICA and Sild unaltered cAMP level significantly (P > 0.05). There were PDE5A1 and PDE5A2 mRNA expressions in rat corpus cavernosum with PDE5A2 being the dominant isoform. ICA could obviously inhibit these two isoforms mRNA expression in rat penis, and decrease PDE5A1 more pronouncedly (P < 0.01). The present study indicated that the aphrodisiac mechanisms of icariin involved the NO-cGMP signal transduction pathway, with increasing cGMP levels in the corpus cavernosum smooth muscle. The inhibitory effect of icariin on PDE5 mRNA expression, especially on PDE5A1, might account for its molecular mechanisms for its long-term activity.


    Asian J Androl. 2003 Mar;5(1):15-8. Links
    Effects of icariin on cGMP-specific PDE5 and cAMP-specific PDE4 activities.


    * Xin ZC,
    * Kim EK,
    * Lin CS,
    * Liu WJ,
    * Tian L,
    * Yuan YM,
    * Fu J.

    Department of Urology, the 1st Hospital, Peking University, 8 Xishiku Street, Xicheng District, Beijing 100034, China. xinzc@bjmu.edu.cn

    AIM: To clarify the mechanism of the therapeutic action of icariin on erectile dysfunction (ED). METHODS: PDE5 was isolated from the human platelet and PDE4 from the rat liver tissue using the FPLC system (Pharmacia, Milton Keynes, UK) and the Mono Q column. The inhibitory effects of icariin on PDE5 and PDE4 activities were investigated by the two-step radioisotope procedure with [(3)H]-cGMP/[(3)H]-cAMP. Papaverine served as the control drug. RESULTS: Icariin and papaverine showed dose-dependent inhibitory effects on PDE5 and PDE4 activities. The IC(50) of Icariin and papaverine on PDE5 were 0.432 micromol/L and 0.680 micromol/L, respectively and those on PDE4, 73.50 micromol/L and 3.07 micromol/L, respectively. The potencies of selectivity of icariin and papaverine on PDE5 (PDE4/PDE5 of IC(50)) were 167.67 times and 4.54 times, respectively. CONCLUSION: Icariin is a cGMP-specific PDE5 inhibitor that may be developed into an oral effective agent for the treatment of ED.
  29. Elite Member
    thewilman's Avatar
    Stats
    5'9"  165 lbs.
    Join Date
    Jun 2004
    Age
    44
    Posts
    6,167
    Rep Power
    3209
    Level
    50
    Lv. Percent
    95.33%
    Achievements Activity ProPosting ProPosting Authority

    Thumbs up


    I'm in! Thanks guys!

  30. New Member
    xjsynx's Avatar
    Join Date
    Apr 2006
    Posts
    92
    Rep Power
    175
    Level
    67
    Lv. Percent
    74.66%
    Achievements Activity ProActivity Authority

    Hook a brotha up...
  31. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by Lanbane View Post
    Hey D,

    I RPM'ed this morning.... 4 caps.
    Did bodyweight dips as my first tricep exercise. Set of 20... felt good.... so I did a set of 30..... felt good..... then a set of 50 which felt awesome, before moving on to skullcrushers, 3 sets of 10 with 100 lbs.

    Damn good numbers for me!!! Me lovey RPM long time.
    RPM'ed- nice adaptation of supplement terminology!!!!

    As for the workout- WOW!!

    I haven't done arms in months- I squat 3-4x a week, clean, pull, deadlift, plus drills, plylos, blocks, and everything else I do w/ track- I look like a friggin' centaur right now- all core, quads, hams, and calves, upper body just isn't what it used to be- trade off though- I am a LOT faster
  32. Applied Nutriceuticals Rep.
    ugab37's Avatar
    Stats
    6'1"  238 lbs.
    Join Date
    Feb 2006
    Posts
    1,013
    Rep Power
    613
    Level
    24
    Lv. Percent
    52.79%
    Achievements Posting Pro

    Ive now decided to marry this product as I know it will contribute more to my life than any woman ever could!!

    Disclaimer: My fiance will probably beat me lifeless after reading that, but if I take 3 yellow caps, Ill probably not feel the pain, so I do have a plan.
  33. AI Sports Fitness Expert
    buffhunk29's Avatar
    Join Date
    Sep 2005
    Age
    30
    Posts
    1,497
    Rep Power
    866
    Level
    28
    Lv. Percent
    99.1%
    Achievements Posting Pro

    This is AWESOME!!!!

    To ANYONE who ordered FREE Samples get ready for the best workouts of your life!! This is NO BULL, this product is like nothing you will have every tried before! You HAVE BEEN WARNED, NO GO SMASH SO WEIGHTS AND TELL US YOUR WHAT YOU THINK!!
  34. Elite Member
    john123131's Avatar
    Join Date
    Mar 2007
    Posts
    5,792
    Rep Power
    2989
    Level
    50
    Lv. Percent
    9.1%
    Achievements Activity ProPosting ProPosting Authority

    ahhhhh...honestly im impressed with all the new companies innovation of new and unique supps coming to the market!!..
  35. AI Sports Fitness Expert
    buffhunk29's Avatar
    Join Date
    Sep 2005
    Age
    30
    Posts
    1,497
    Rep Power
    866
    Level
    28
    Lv. Percent
    99.1%
    Achievements Posting Pro

    So So True John!!!
  36. Elite Member
    john123131's Avatar
    Join Date
    Mar 2007
    Posts
    5,792
    Rep Power
    2989
    Level
    50
    Lv. Percent
    9.1%
    Achievements Activity ProPosting ProPosting Authority

    rms...buff....ill admit i havent gone over the ingredients in too much depth yet, but will there be issues getting this into Canada??
  37. O.G. Appnut
    Vitruvian's Avatar
    Join Date
    Dec 2005
    Posts
    5,203
    Rep Power
    85509
    Level
    49
    Lv. Percent
    31.7%
    Achievements Activity ProPosting ProPosting Authority

    Not addressed to me I know... but there should be no issues whatsoever. If there are, I personally (Not AN) will send you a brand new coffee mug... (mysteriously full of RPM). ;-)
    Applied Nutriceuticals Representative
    Better Results Through Science
    Success occurs when everything you have is APPLIED.
    Have you heard about N.O. Uptake? ;-)
  38. Elite Member
    john123131's Avatar
    Join Date
    Mar 2007
    Posts
    5,792
    Rep Power
    2989
    Level
    50
    Lv. Percent
    9.1%
    Achievements Activity ProPosting ProPosting Authority

    haha..Lane sorry bro...ill throw your name in next time too!!..
    yeah the mug may work if theres issue @ my crazy ass border!
    john
  39. Board Sponsor
    rms80's Avatar
    Stats
    6'2"  215 lbs.
    Join Date
    Jan 2007
    Posts
    4,224
    Rep Power
    204222
    Level
    46
    Lv. Percent
    89.6%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by john123131 View Post
    rms...buff....ill admit i havent gone over the ingredients in too much depth yet, but will there be issues getting this into Canada??
    Shouldn't be any issues- no ph's or ephedra- we are currently working on the paperwork to get a distributorship going in Canada. The laws are a little different from ours- the FDA tends to be a lot stricter than your government about drug-like claims, but far less stringent when it comes to manufacturing standards and the types of product contituents used- FDA really does not make a whole lot of sense on this one.....

    Bottom line: We can actually legally make more claims in Canada than in the USA about our products, and long as they are found up to code by your governing body- we are preparing a presentation to submit them soon for approval- wish us luck!!!
  40. Elite Member
    john123131's Avatar
    Join Date
    Mar 2007
    Posts
    5,792
    Rep Power
    2989
    Level
    50
    Lv. Percent
    9.1%
    Achievements Activity ProPosting ProPosting Authority

    good luck...Ill be the "official Canadian Tester"..of a bottle of course!! =)
  

  
 

Similar Forum Threads

  1. Replies: 30
    Last Post: 01-06-2009, 06:02 PM
  2. A little humor for the over 30's
    By AE14 in forum General Chat
    Replies: 9
    Last Post: 09-29-2008, 06:39 PM
  3. Id like to thank nutraplanet for the hook ups
    By Cellardude in forum Nutraplanet
    Replies: 7
    Last Post: 02-22-2008, 04:35 PM
  4. Thanks for the hoop-up NP!!
    By Hyde12 in forum Nutraplanet
    Replies: 25
    Last Post: 10-29-2007, 05:39 PM

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
Log in
Log in