Melatonin blocks the activation of estrogen receptor for DNA binding

  1. Melatonin blocks the activation of estrogen receptor for DNA binding


    Found this to be extremely interesting!!!


    Melatonin blocks the activation of estrogen receptor for DNA binding
    AVELINA GARCÍA RATO*1, JUANA GARCÍA PEDRERO*, M. ARÁNTZAZU MARTÍNEZ{dagger}, BEATRIZ DEL RIO, PEDRO S. LAZO* and SOFÍA RAMOS*

    * Departamento de Bioquímica y Biología Molecular, Universidad de Oviedo, 33006 Oviedo, Spain; and
    {dagger} Hospital de Jove, 33212 Gijón, Spain

    1Correspondence: Departamento de Bioquímica y Biología Molecular. Facultad de Medicina. Universidad de Oviedo, 33006 Oviedo. Spain. E-mail: SRG@sauron.quimica.uniovi.es

    The present study shows that melatonin prevents, within the first cell cycle, the estradiol-induced growth of synchronized MCF7 breast cancer cells. By using nuclear extracts of these cells, we first examined the binding of estradiol–estrogen receptor complexes to estrogen-responsive elements and found that the addition of estradiol to whole cells activates the binding of the estrogen receptor to DNA whereas melatonin blocks this interaction. By contrast, melatonin neither affects the binding of estradiol to its receptor nor the receptor nuclear localization. Moreover, we also show that addition of estradiol to nuclear extracts stimulates the binding of estrogen receptor to DNA, but this activation is also prevented by melatonin. The inhibitory effect caused by melatonin is saturable at nanomolar concentrations and does not appear to be mediated by RZR nuclear receptors. The effect is also specific, since indol derivatives do not cause significant inhibition. Furthermore, we provide evidence that melatonin does not interact with the estrogen receptor in the absence of estradiol. Together, these results demonstrate that melatonin interferes with the activation of estrogen receptor by estradiol. The effect of melatonin suggests the presence of a receptor that, upon melatonin addition, destabilizes the binding of the estradiol–estrogen receptor complex to the estrogen responsive element.
    PharmD


  2. Interesting.
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  3. funny this post today, as I just took melatonin for the first time last night. Woke up refreshed, not groggy....but had a ****ing weird dream last night. I dreamt that one of my bosses was a teenage girl, and that our accountant was.....shirtless and hairy.
    E-Pharm Nutrition Representative

  4. Quote Originally Posted by rampage jackson
    funny this post today, as I just took melatonin for the first time last night. Woke up refreshed, not groggy....but had a ****ing weird dream last night. I dreamt that one of my bosses was a teenage girl, and that our accountant was.....shirtless and hairy.

    LOL dude, this is so awesome. I wonder what happends if you take 20 mg melatonin, along with a eca stack, that way you will not feel sleepy, and have more test? Maybe a rep will see that and make a test booster with melatonin. Anyone took some in the morning?

  5. Thanks LMD, Good info You deserve a for that one

  6. :hot: :donut: :burg: izza: and Haha

  7. Weird i get mental dreams on it too.

  8. Quote Originally Posted by FitnFirm
    Thanks LMD, Good info You deserve a for that one
    Haha I SOOO wish I could eat that. I am currently licking the screen. But what I wont tell you is whether it is of your avatar or the burger

    DAMNIT, sorry again Viper haha
    PharmD
  

  
 

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