In general, P-Slin can have a half-life of up to six hours.
First, some background. As is well-known, the pattern of GLUT-4 activation and deactivation (when GLUT-4 proteins revert to the cytoplasm) depend on the initial source of the trigger or stimulus. This trigger or stimulus can be insulin, exercise (muscle contractions), or Anabolic Pump/P-Slin. In particular, Anabolic Pump/P-Slin activate GLUT-4 completely independent of exercise and insulin; exercise activates GLUT-4 via 5' Adenosine Monophosphate-activated Protein Kinase (AMPK) [actually via increased GLUT-4 mRNA gene transcription], while insulin stimulates GLUT-4 via binding on and activation of insulin receptors. GLUT-4 transport proteins are at the core of glucose disposal. Like GLUT-1, GLUT-2, GLUT-3, and GLUT-5, GLUT-4 is technically a hexose transporter that effectively transports hexose down a concentration gradient. As it turns out, cellular glucose uptake occurs via facilitated diffusion through these so-called hexose transporters.
Now to GLUT-4 half-life. Insulin is metabolized in the liver and has a relatively short half-life of about five minutes in a normal individual. Consequently, insulin-induced GLUT-4 translocation is acute and corresponds to rapid glucose disposal (and, depending on carbohydrate amount, can trigger post-prandial oxidative stress). Exercise-induced GLUT-4 translocation falls off rapidly after cessation of physical exertion. The Anabolic Window of Opportunity here is generally regarded as ninety minutes, although GLUT-4 translocation may last a while longer. In general, though, GLUT-4 is estimated to have a half-life of between six and ten hours. This is why taking up to three capsules of Anabolic Pump daily or a few doses of P-Slin can, in principle, sustain a steady-state maintenance of GLUT-4 action. Furthermore, even though GLUT-4 proteins may have a half-life of more than six hours, glucose disposal would not be equally effective with repeated consumption of equivalent amounts of carbohydrates within this interval. At best, carbohydrate consumption should be tapered off within this time period.
The recommended fifteen to twenty minutes waiting period between Anabolic Pump/P-Slin intake and carbohydrate-containing macronutrient consumption, by the way, is important to ensure initial translocation of GLUT-4 from the cytoplasm to the cell membrane. This timing is a guide. For some, thirty minutes would be a more effective window. So this, just like the appropriate post-Anabolic Pump/P-Slin carbohydrate intake, may require some experimentation. Yet, while it is doubtful there are any advantages in waiting beyond thirty minutes post-Anabolic Pump/P-Slin intake before consuming carbohydrate-containing meals, it may be unavoidable from time to time to wait longer than thirty minutes. Such once-in-a-while incidences would not negate the overall effects of Anabolic Pump/P-Slin. As is obvious, GLUT-4 translocation has little pharmacological relevance in the absence of carbohydrates/glucose. Consequently, once GLUT-4 transport proteins are activated (within fifteen to thirty minutes of Anabolic Pump/P-Slin intake), it is absolutely important to consume carbohydrates to take advantage of this biological event. In the absence of glucose, GLUT-4 proteins may revert to the cytoplasm. When this happens depends on different factors, and the process may set in gradually after about one hour to ninety minutes post-GLUT-4-translocation.
