Scientifically Proven Natural Testosterone Boosters

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    Scientifically Proven Natural Testosterone Boosters


    Hey all, first post.

    I am just wondering if there are any scientifically proven natural test boosters other than Novedex XT.
    (ncbi.nlm.nih.gov/pubmed/17460335)

    The results are pretty good for this supplement but apparentley the lack of estrogen lowers your libido. As a male in my early 20s I definitely don't want that, but I will add that my ratio probably isn't as good as some of you so maybe it won't affect me.

    I have just started taking Isatori Isa Test but I have read there is no scientific proof backing up it's claims and tests show hormone levels stay the same. I am only on my third day and I feel like my mood and energy levels are slightly better, but I am starting to think that maybe I wasted my money on what is essentially snake oil.

    tl;dr: What are some scientifically proven natural testosterone boosters?

    The studies need to be published in peer-reviewed journals or they may as well be fake.

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    I would say that you could take blood work as proof that test boosters work as well so you can include Bioforge, DTHC and Formadrol as proven.

    I've used Bioforge and DTHC and they definitely work for me but have yet to try Formadrol.
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    This is the source of my claim that Isa Test doesn't alter hormone levels:
    blog.ultimatefatburner.com/2008/06/15/the-test-test-results/

    Considering all the anecdotal evidence that it works, I was surprised to read this. I'm still going to do the full bottle and see how I feel at the end of it before I make my mind up.
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    Quote Originally Posted by warsteiner View Post
    I would say that you could take blood work as proof that test boosters work as well so you can include Bioforge, DTHC and Formadrol as proven.

    I've used Bioforge and DTHC and they definitely work for me but have yet to try Formadrol.
    Bioforge sounds interesting, I've never heard of Forskolin before.
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    Not everybody still has theirs...wait, wut?
    RcB Since 09-06-2011 20:55 EST, Post 49
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    Quote Originally Posted by stxnas View Post
    Not everybody still has theirs...wait, wut?
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    Quote Originally Posted by stxnas View Post
    Not everybody still has theirs...wait, wut?
    Quote Originally Posted by JeepBeat View Post
    LOL, lots of people write foreskin rather than forskolin, which is what stxnas was referring to.
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    Quote Originally Posted by warsteiner View Post
    LOL, lots of people write foreskin rather than forskolin, which is what stxnas was referring to.
    Ohh...now I get it.
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    ^^^^Id give Formadrol Extreme a try. The planet has a great deal right now. I love this stuff!

    RECOVERBRO


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    Quote Originally Posted by JoHNnyNuTZ View Post
    ^^^^Id give Formadrol Extreme a try. The planet has a great deal right now. I love this stuff!
    Is this better than Novedex XT?

    Cause it is basically an AI like XT.
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    MMM. Better? Well Ive tried novadex xt and didnt get that great of results. Formadrol ex, ive tried several times, and absolutely loved it, and have 2 bottles waiting for me when I get done running FREETEST. I must also include FREE TEST! This stuff has been amazing since the first couple of days. Grade A product IMHO!!

    RECOVERBRO


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    Quote Originally Posted by JeepBeat View Post
    Hey all, first post.

    I am just wondering if there are any scientifically proven natural test boosters other than Novedex XT.
    (ncbi.nlm.nih.gov/pubmed/17460335)

    The results are pretty good for this supplement but apparentley the lack of estrogen lowers your libido. As a male in my early 20s I definitely don't want that, but I will add that my ratio probably isn't as good as some of you so maybe it won't affect me.

    I have just started taking Isatori Isa Test but I have read there is no scientific proof backing up it's claims and tests show hormone levels stay the same. I am only on my third day and I feel like my mood and energy levels are slightly better, but I am starting to think that maybe I wasted my money on what is essentially snake oil.

    tl;dr: What are some scientifically proven natural testosterone boosters?

    The studies need to be published in peer-reviewed journals or they may as well be fake.
    youve hit it on the head. with any supplement which is herbal , a lot of the feedback is anecdotal and most likely has a place in folk lore medicine

    the local health food store is full of these supplements for all types of ailments which some people swear they work but there is no scientific evidence to back them up.

    ive looked at the ingredients of test x2 and going through them -the zinc b6 and magnesium looks like a copy of zma -whether that increases test is debatable, testofen -well it seems to work .tribulus seems to be overated and doesnt work imo, Then you have horny goat weed with icarin which is one of those debatable supplements,

    as for the rest -you have an estrogen clearing agent . and some Ajuga Turkestancia
    Ajuga Turkestancia contains the compound Turkesterone, and is a popular natural anabolic product in the former USSR. Taken with Rhaponticum Carthamoides has been shown to increase strength, but wheres the studies?

    Its frustrating when you spend your hard earned cash and dont get anything back
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    Quote Originally Posted by JeepBeat View Post
    Hey all, first post.

    I am just wondering if there are any scientifically proven natural test boosters other than Novedex XT.
    (ncbi.nlm.nih.gov/pubmed/17460335)

    The results are pretty good for this supplement but apparentley the lack of estrogen lowers your libido. As a male in my early 20s I definitely don't want that, but I will add that my ratio probably isn't as good as some of you so maybe it won't affect me.

    I have just started taking Isatori Isa Test but I have read there is no scientific proof backing up it's claims and tests show hormone levels stay the same. I am only on my third day and I feel like my mood and energy levels are slightly better, but I am starting to think that maybe I wasted my money on what is essentially snake oil.

    tl;dr: What are some scientifically proven natural testosterone boosters?

    The studies need to be published in peer-reviewed journals or they may as well be fake.
    Isatori Isa Test has Testofen I used 2 bottles and the stuff is amazing really Great!!!! but for 26 days for $40+ is a little expensive
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    Quote Originally Posted by corsaking View Post
    youve hit it on the head. with any supplement which is herbal , a lot of the feedback is anecdotal and most likely has a place in folk lore medicine

    the local health food store is full of these supplements for all types of ailments which some people swear they work but there is no scientific evidence to back them up.

    ive looked at the ingredients of test x2 and going through them -the zinc b6 and magnesium looks like a copy of zma -whether that increases test is debatable, testofen -well it seems to work .tribulus seems to be overated and doesnt work imo, Then you have horny goat weed with icarin which is one of those debatable supplements,

    as for the rest -you have an estrogen clearing agent . and some Ajuga Turkestancia
    Ajuga Turkestancia contains the compound Turkesterone, and is a popular natural anabolic product in the former USSR. Taken with Rhaponticum Carthamoides has been shown to increase strength, but wheres the studies?

    Its frustrating when you spend your hard earned cash and dont get anything back

    Is True bud but remember NOT ALL EXTRACTS ARE THE SAME and NOT ALL GUYS RESPOND EQUAL

    The only Tribulus Terristris thtas works is (extracted for tribulosin)
    PureSap™ (Extract engineered Sapogenin/Alkaloids from Chlorophytum)
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    TCF-1 or Testforce. Real human studies. DAA. Right now my vote goes to TCF-1. Saw a video where testforce didnt mix as well as everyone said it would and also the user said it tasted like dirty lake water. No thanks.
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    idk if pink magic has some science but looks good
    The difference between who you are and who you want to be is what you do.
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    Quote Originally Posted by rochabp View Post
    idk if pink magic has some science but looks good
    Pink Magic's ingredients have positive effects on function of the testes, as proven in a study.

    DAA, the ingredient in TCF-1 by Primordial, has also been shown in a study to give a dramatic increase in natural test. It is the new buzz supplement for test production.
    Just inject.
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    Safed Musli
    Testofen
    Nettle
    Forskolin

    That's all I can think of that have studies in humans. Most data, for better or worse, is from rat studies because they're cheaper and easier to control.
    M.Ed. Ex Phys
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    Quote Originally Posted by Rodja View Post
    Safed Musli
    Testofen
    Nettle
    Forskolin

    That's all I can think of that have studies in humans. Most data, for better or worse, is from rat studies because they're cheaper and easier to control.
    DAA was a human study as well.
    Just inject.
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    Quote Originally Posted by HereToStudy View Post
    DAA was a human study as well.
    I knew I forgot something. I'm still in my post-WO lull.
    M.Ed. Ex Phys
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    Normally I don't bother with this stuff because, well quite frankly, I think that 99.99% of it is goat caca. However the thread title got me interested in what sort of science is actually used.

    So for sh!ts and giggles I googled 'Testofen study' and came up with this: http://forum.bodybuilding.com/attach...hmentid=776901... (if there is a different one please let me know)

    From a science perspective this is a sorry excuse for a study and is basically just another form of 'infomercial.' I love this line:

    Free Testosterone levels in serum were estimated before and after 8-week consumption
    of TESTOFEN.


    IMO this wording basically says that there is no actual certainty in the product's effectiveness and gives the company a solid defense in the event of any legal challenge.

    The amount of deception in the supplement industry never ceases to amaze me.
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    I've been taking tcf-1 for 12 days now, here are my results: libido increased greatly, acne on back 10x what it normally is, aggression increased slightly but not very much, mood improved 5/10, strength increasing nicely. I do not have blood work so I cannot say it has increased my test, but the effects I am seeing are indicative of increased test. Similar to some of the old school ph's like original superdrol as far as side effects, except no calf pumps thank god.
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    I think this one actually works (doc says so!): Testemax





    Sorry couldn't resist
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    Here is a decent read if you are interested

    Applied Nutriceuticals® In-House Pilot Work, April 2010,
    Copyright © 2010 by Applied Nutriceuticals®

    The Effects of a Dietary Supplement, Free Test™, on Serum Free and Total Testosterone Levels in Weight-Trained Male Subjects

    Tanis, D., Orrell, D. and Long, Dr. W.

    Lab Corp® and Applied Nutriceuticals® Research, Charlotte, North Carolina 28269

    Address all correspondence to: Dirk Tanis, Jr., MSci, Department of Research, Applied Nutriceuticals®, 8112 Statesville Road Suite G, Charlotte, NC 28269.

    Abstract
    The objective of many weight-trained men is to raise circulating testosterone levels, both through training, and supplementation. In light of these facts, a dietary supplement formulation, Free Test™, was created, combining 3,7-Keto DHEA, Acetyl-L-Carnitine, N-Acetyl-Cysteine, Coleus Forskolii (Forslean®), Resveratrol, Quercetin Dihydrate, B-Vitamins, Magnesium, Zinc, Bioperine®, and Selenium. The product focuses on decreasing the homeostatic mechanisms that lower endogenous testosterone production, while at the same time allowing for sustained, safe increases in free and serum testosterone levels. The purpose of this study was to determine the effects of Free Test™ on serum hormone levels in resistance trained males. Methods: 6 men (mean age, 32.5 yr. ± 10.01, range 18–49 yr) with at least 4 years of weight-training experience were studied. Total (TT) and free (FT) testosterone levels were measured via blood work (LabCorp®) on two separate occasions before (Pre) and after (Post) having taken the dietary supplement, with serum and free testosterone being measured on days (d)= Day 0 and Days 21-61 (mean time period 31.6 d ± 14.87, range 21-61 d). One (1) blood draw per analysis period was taken; Two (2) total draws per subject over two separate days, and during the trial period, subjects were given a 4 capsule daily dosage of the supplement. Results: Each of the 6 weight-trained men (100%) had significant increases in free testosterone levels after 21-61 days of ingesting a 4 capsule daily dosage of Free Test™ when compared to baseline values. Average baseline FT was 9.55 pg/mL ± 1.80 (SD). Average FT following administration of the supplement was 16.98 pg/mL± 5.2, a 78% increase in FT (p<0.10) values for the group.

    Introduction
    Numerous studies have documented the anabolic effects of testosterone on skeletal muscle, and this has led to a huge increase in dietary supplement products on the current market that claim to raise testosterone levels and improve body composition. Many testosterone-enhancing supplements have been shown to be either ineffective or dangerous, due to various factors. Some of these products do indeed increase testosterone, but many of the most effective products (Anabolic Steroids and Prohormones) in turn have the most unsafe side effects. Prohormones (PH) and AAS are extremely effective in increasing androgen levels, as they directly bind the androgen receptor (AR), which is the most direct way of increasing testosterone levels. However, as aforementioned, these products can also cause some deleterious side effects, and recently the United States FDA has cracked down on the manufacture of this type of products, and has openly and actively pursued product recalls against companies that distribute prohormones. Similarly, many non-PH and AAS-based testosterone boosters also exist on the current market. Unfortunately, many of these products are completely ineffective, and use a hodgepodge of low extraction-percentage herbals, ineffective or unproven anabolic compounds and/or amino acids. Most of these products use a “kitchen sink” formula, and claim that their combination is “scientifically proven” to work. Some herbal components boost testosterone levels, but most of the time, it is only when taken in completely unattainable dosages that would make a normal person extremely sick or make the product outrageously expensive to produce.

    Therefore, an opportunity exists to create a product that can provide a safe way to increase testosterone levels, while being compliant with the Dietary Supplement and Health Act (DSHEA) and other current United States regulatory guidelines. The goal of Free Test™ was to create a formula that focuses on decreasing the homeostatic mechanisms that lower endogenous testosterone production, while at the same time allowing for sustained increases in testosterone, and its positive subsequent effects on lean body mass. The formulation is intended to work not by stimulating the androgen receptor (AR) and having direct androgenic activity, but by manipulating factors that can increase or decrease TT and FT in the body. TT is nearly 100% bound by several different factors- 40% is bound by Steroid Hormone Binding Globulin (SHBG, a β-Globulin that binds hormones such as testosterone and estrogen), 40% is bound by albumin, and 17% is bound by other proteins. The remaining testosterone is considered to be FT, or the unbound, biologically active component of testosterone (TST). Once in the blood, FT can be bound by the AR, and converted to dihydrotestosterone (DHT), or converted to estradiol, via the aromatase enzyme (29).

    The product formulation seeks to increase FT via several different indirect means:
    • Via Improving the Testosterone:Estrogen (T:E) profile and concurrently increasing testosterone levels through using a naturally-occurring aromatase inhibitor (21-22,27)
    • Through Increasing Nitric Oxide (NO) and cyclic guanosine monophsophate ( cGMP) levels, which lead to increases in luteinizing hormone (LH), which is a trigger for greater endogenous testosterone production (1-4,15, 27-28).
    • Lowering levels of certain cytokines- many cytokines are inflammatory agents that can inhibit endogenous testosterone production by reducing levels of lutenizing hormone (LH) and decreasing testicular function (25-26,33-34)
    • Increasing cyclic adenosine monophosphate ( cAMP) levels, which also increase LH levels (14, 16,17)

    The purpose of this pilot work is twofold, in that it allows the researchers to establish that the product does indeed increase TT and FT in a directional manner, and can be used safely for varying periods of time (measured via questionnaire).

    Methods

    Subjects

    Six men between the ages of 18 and 49 yr (mean age, 32.5 ± 10.01) were studied. Eight men were initially chosen; however, one patient had to be excluded because of a pre-existing history of thyroid dysfunction, and another had to be excluded due to the use of an anabolic substance 4 weeks prior to the test. No patients had a history of glucocorticoid or anticonvulsant use, diabetes mellitus, gastrointestinal disease, gastrointestinal surgery, acromegaly, malignancy, or any other known metabolic disease. No patient had a history of alcoholism. All subjects were required to have at least 4 years of weight training experience. There was no history of pubertal or adult testosterone deficiency or growth disturbance, no history of delayed puberty, and no history of pituitary disease or deficiency. As aforementioned, subjects who had used various prohormone derivatives or pharmacologic agents such as anabolic steroids within 6 weeks prior to the study were not allowed to participate. All subjects gave written informed consent, and a questionnaire was given to all subjects regarding how well they tolerated the supplement, protocol for the trial, and also a section where any adverse events or symptoms could be reported (29,31,37).

    List of Exclusionary Substances
    Refer to WADA 2009 Guidelines for entire list (35)


    Testing Sessions

    There were two separate days of testing: Baseline Testing Day (Pre), where each subject had TT and FT measured via blood draw, and Testing Day 2 (Post), where subjects had FT and TT measured via blood draw after having received a 4 capsule daily dosage of the product over a varying time interval (Range 21-61 days). The subject questionnaire was returned after the second blood draw for each subject (29,31,37).

    Blood Collection and Assay Methods

    Blood samples were taken from an antecubital vein into a 10 ml collection tube, with a 2 mL sample. The samples were handled via Lab Corp® protocol 140103, being allowed to stand at room temperature for 10 min and then centrifuged. The serum was removed and frozen for later analysis. Measured via Lab Corp® protocol 140103 Testosterone,Free (Direct), Serum with Total Testosterone. Routine serum biochemical measurements were made using standard techniques. FT was measured by direct analog/ radioimunnoassay (RIA), and TT was measured by Electrochemiluminescence Immunoassay (ECLIA). FT values were measured in pg/mL; TT values were measured in ng/dL (36).

    Supplementation Protocol
    Subjects were randomly assigned differing lengths of trial supplementation for the product. Two subjects were assigned a 20-22 day trial, three subjects were assigned a 28-30 day trial, and one subject was assigned a 60-62 day trial. The reasoning behind the variable length trials between subject groups was to see if the formulation was sound for elevations in TST over longer, medium, and shorter duration, and to get an idea of whether or not the supplement could be used safely and effectively over varying periods of time. Subjects were instructed to orally ingest 4 capsules upon waking in the AM, preferably with food. During the supplementation period, the physical activity and diet of the subjects was not monitored. Instead, each participant was instructed not to change any of their dietary habits and physical training regime during the trial period (18-19,29,31,37).

    Reported Side Effects from Trial

    After completing their final blood draw, subjects reported by questionnaire any issues they may have had with the supplementation or the protocol. Subjects were also asked to list any kind of medical side effects that might have occurred during the research.

    Statistical Analysis
    Due to the nature of pilot research (low number of subjects and only two variables measured)- the design of the study is a quasi-experimental One Group Pretest-Posttest Design. Therefore, a t-test paired for pretest-posttest scores for both TT and FT was utilized. All statistical procedures were performed using GraphPad software and a probability level of p< 0.10 was adopted throughout the study (37).
    Dirk Tanis, BA, MSci
    Chief Operating Officer, Applied Nutriceuticals
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    Results

    Posttest Subject Questionnaire

    Subjective analysis of the posttest subject questionnaire found that all 6 participants appeared to have exhibited full adherence to the supplement protocol, and were able to finish the required dosing regimen and testing procedures with no side effects. Evaluation of the questionnaires also revealed no noticeable changes in dietary habits or physical activity levels during the trial period.

    Serum FT and TT Levels

    Compared to baseline, posttests showed FT levels to be significantly higher, indicating a significant main effect for FT (p = 0.092). TT levels increased, but the increases were not enough to be deemed significant (p= 0. 1484) (37).

    Table 1: FT Levels Baseline v. Post
    Group Baseline FT FT After Free Test™ Supplementation
    Mean 9.550 pg/mL 16.983 pg/mL
    SD 1.807 5.204
    SEM 0.738 2.125
    N 6 6

    Table 2: TT Levels Baseline v. Post
    Group Baseline TT TT After Free Test™ Supplementation
    Mean 436.67 ng/dL 649.83 ng/dL
    SD 200.60 266.31
    SEM 81.90 108.72
    N 6 6



    Table 3: Individual Values By Subject- TT
    Subject
    Age
    Duration of Usage
    Baseline TT TT Post
    1 29 yr 21 d 450 ng/dL 998 ng/dL
    2 34 yr 21 d 306 ng/dL 425 ng/dL
    3 18 yr 61 d 689 ng/dL 983 ng/dL
    4 34 yr 29 d 321 ng/dL 516 ng/dL
    5 31 yr 28 d 196 ng/dL 458 ng/dL
    6 49 yr 30 d 658 ng/dL 519 ng/dL


    Table 4: Individual Values By Subject- FT
    Subject
    Age
    Duration of Usage
    Baseline TT TT Post
    1 29 yr 21 d 11.7 pg/mL 25.3 pg/mL
    2 34 yr 21 d 10.4 pg/mL 13.0 pg/mL
    3 18 yr 61 d 11.0 pg/mL 17.1 pg/mL
    4 34 yr 29 d 9.3 pg/mL 18.3 pg/mL
    5 31 yr 28 d 7.5 pg/mL 18.1 pg/mL
    6 49 yr 30 d 7.4 pg/mL 10.1 pg/mL



    Discussion

    In this research, we sought to find the effects of Free Test™ supplementation at 4 capsules per day over varying time periods on TT and FT. No adverse side effects were reported by the subjects, and subjective data gathered from posttest questionnaires found that Free Test™ was well-tolerated over time periods ranging from three to nine weeks.
    In reference to changes in TT, while subjects did show a directional increase, but this increase was not significant (20). The significant change over the course of the pilot was in FT, which underwent an increase of 78%. The increases in FT were most likely related to several different mechanisms of action in the product. Free Test™ contains Acetly-L-Carnitine, which has been shown to increase nitric oxide (NO) and cyclic GMP (cGMP) levels via elevating acetylcholine levels. NO is important in that it regulates vascular tone, CNS stimulation, and, most importantly, induces the release of luteinizing hormone releasing hormone (LHRH) and regulates cyclic guanosine monophosphate (cGMP) levels. NO also activates the release of LHRH which reaches the pituitary and activates the release of luteininzing hormone (LH) via the activation of neural NO synthase (NOS) in the pituitary gland (17,18,30,32).
    Similarly, the fact that the product increases the action of NO and cGMP are important one, as both entities have a stimulatory action on steroidogenesis via increased LH production. Therefore, high cGMP levels also equate to high levels of LH, and when cGMP levels are elevated, it serves as an intermediate in the signaling cascade that ranges from luteinizing hormone (LH) binding to testosterone production. In numerous pathway studies, increases in cGMP increased phosphorylation of the steroidogenic acute regulatory protein (StAR). Steroidogenic acute regulatory protein (StAR) is a Leydig cell cholesterol transfer protein that provides the building blocks for testosterone synthesis. StAR activation is necessary for the stimulation of steroidogenic enzymes involved in the transfer of cholesterol to testosterone. These results suggest that cGMP contributes to the control of basal steroidogenesis (endogenous testosterone production) in Leydig cells through the PKG-dependent modification of the StAR protein and interaction.with LH. Even more important is the fact that LH, via receptors found on the surface of Leydig cells, controls the production and secretion of testosterone. The subsequent binding of LH with its receptor allows signalling through the cyclic AMP pathway via GTP binding proteins. Signal transduction occurs through the protein kinase A pathway as its principal signal transduction mechanism, and this ultimately allows for the release of testosterone after 30-60 minutes of LH stimulation. This is most likely one of the mechanisms that contributed to increased FT (1-3,15-16,32).
    Another contributing factor to increased FT has to do with This occurs primarily through elevation of 3,5 cyclic adenosine monophosphate (cAMP), a second messenger important in hormone signaling, via forskolin. One study (Bristow et al, 1984) showed that forskolin was able to increase cAMP levels 4.82 times more than a placebo. Increased cAMP production is responsible for the activation of protein kinase A, which is an enzyme that has positive effects androgen receptor binding even in the absence of other androgens. Increased cAMP also is a signal for steroidogenesis (testosterone production) in the Leydig cells of the testes, by increasing levels of steroidogenic acute regulatory protein (StAR- as mentioned above). By this process, and the fact that high cAMP levels also equate to high levels of luteinizing hormone (the hormone responsible for mediating endogenous spermatogenesis), significant increases in endogenous testosterone production may occur, along with a resulting increase in anabolism and protein synthesis. A 2005 study in The Journal of Obesity Research found that obese men taking 250 mg of 10% forskolin a day for 12 weeks (roughly the dosage included in the daily dosage of Free Test™) experienced an averaged 33% increase in free testosterone levels, averaged a 10 lbs. fat loss per person and increased lean mass an average of 8 lbs (5-12).
    The product also seems to have a strong anti-oxidative effect on the testes, which can allow the testes an optimal environment for testosterone production. The combination of N-Acetyl-Cysteine and selenium has been shown in recent studies to cause elevations in FT. Glutathione is a tripeptide thiol found in all cells of the body, and is responsible for regulating protein synthesis and detoxifying cell structures. Selenium is needed for the detoxifying enzyme glutathione peroxidase, and NAC significantly increases glutathione levels. Increases in glutathione and glutathione peroxidase seem to be negatively correlated with cytokine release- as levels of these anti-oxidants increase, cytokine levels decrease, and several types of cytokines have been linked to testicular impairment via decreasing LH levels. This effect is readily apparent in the male reproductive system, as several studies have shown that NAC can reverse testicular damage cross-indicated with cytokine release, and cytokine-related testicular suppression. This alone can allow for conditions that are extremely favorable for optimal testosterone production (13,25-26,33).
    The increase in FT levels may also be linked to the inclusion of a natural aromatase inhibitor in the product. 3-Desoxy 7-Keto DHEA has demonstrated strong ability to lower estrogen, as It has shown a high binding affinity (Ki value = 0.22 mM) to the aromatase enzyme, and binds in an irreversible manner. This inhibition allows for the production of less estradiol (E2) and estrone (E1) and allows the user of the compound to maintain a higher level of testosterone- a balance referred to as the Testosterone:Estrogen (T:E) ratio. 3,7-Keto DHEA is unique from other commonly available aromatase inhibitors on the sports supplement market, in that it is a natural metabolite of 7-Keto DHEA, and cannot directly bind to the androgen receptor (AR). 3,7-Keto DHEA (like 7-Keto DHEA) also cannot convert to testosterone, estrogen, or progesterone via any type of enzymatic reaction, so it cannot be considered a prohormone.
    Another recent problem with commonly available aromatase inhibitors on the supplement market is the direct conversion of the compound to a controlled substance in the body or during synthesis, either in trace amounts, or full-scale conversion. This does not occur with 3,7-Keto DHEA, as it is formed naturally under various conditions in humans from 7-Keto DHEA and can be readily found in humans in the amount of 5-7 ug/day. The mechanism through which aromatase inhibitors raise testosterone is fairly simple- the HPTA senses low levels of estrogen, and because the body seeks to maintain homeostasis (it likes to maintain at least some estrogen, even in men), there is a concurrent increase in the amount of testosterone that is being produced, as a way to compensate for the low estrogen levels. The increased testosterone levels normally will result in increased estrogen, since there is no estrogen being produced but the brain is essentially tricked into trying to produce more estrogen, so it releases more LHRH and subsequently LH, leading to even higher testosterone levels (23-24).

    Contributions
    DT designed the study, drafted the manuscript, supervised coordination and data acquisition, and performed the statistical analysis. DO and DL participated in the data acquisition.



    Acknowledgments
    We thank LabCorp® for assisting with the assays and blood work.


    References
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    2. Aghdasi B, Reid MB, and Hamilton SL. (1997) Nitric oxide protects the skeletal muscle Ca2+ release channel from oxidation induced activation. J Biol Chem 272:25462-25467

    3. Balon TW, Nadler JL. (1997)Evidence that nitric oxide increases glucose transport in skeletal muscle. J Appl Physiol. 1997 Jan;82(1):359-63.

    4. Etgen GJ Jr, Fryburg DA, Gibbs EM. (1997) Nitric oxide stimulates skeletal muscle glucose transport through a calcium/contraction- and phosphatidylinitric oxidesitol-3-kinase-independent pathway. Diabetes Nitric oxide; 46(11):1915-9.

    5. Asano Tsuguyoshi (2001). Clinical report on root extract of perilla plant (Coleus forskohlii) ForsLean ® in reducing body fat. Asano Institute. Tokyo , Japan.

    6. Kreider RB, et al. Effects of Coleus forskohlii supplementation on body composition and markers of health in sedentary overweight females. Experimental Biology 2002 Late Breaking Abstracts. LB305: 2002.

    7. LEAMON, KB; PADGETT, W; DALY, JW. "Forskolin: Unique diterpene activator of adenylate cyclase in membrane and intact cells" Proc. Natl. Acad. Sci. USA 1981,78,3363-67

    8. DE SOUZA, NJ; DOHADWALLA, AN; REDEN, J; Forskolin, A. "Labdane Diterpenoid with Antihypertensive, Positive Inotropic, Platelet Aggregation Inhibitory, and Adenylate Cyclase Activating Properties" Medicinal Research Reviews 1983, 3(2), 201-219

    9. Badmaev, V. Majeed, M. Conte, A, Parker, J. “Diterpene Forskolin (Coleus forskohlii, Benth.): A possible new compound for reduction of body weight by increasing lean body mass” Sabinsa Corporation http://www.b5srl.com/articles/nu/200...pr/badmaev.htm 1/21/2004

    10. Ding X, Staudinger J. Induction of Drug Metabolism by Forskolin, the Role of The Pregnane X Receptor and the PKA Signal Transduction Pathway. J Pharmacol Exp Ther. 2004 Sep 30

    11. Forskolin, a diterpene compound isolated from the roots of Coleus forskohlii, activates adenylate cyclase in membranes from a variety of mammalian tissues. adenylate cyclase in human basophils and mast cells. Biochem Pharmacol. 1987 Jan 1;36(1):13-20.
    12. Asif AR, Ljubojevic M, Sabolic I, Shnitsar V, Metten M, Anzai N, Müller GA, Burckhardt G, Hagos Y. Regulation of steroid hormone biosynthesis enzymes and organic anion transporters by forskolin and DHEA-S treatment in adrenocortical cells. Am J Physiol Endocrinol Metab. 2006 Dec;291(6):E1351-9. Epub 2006 Jul 11.

    13. Tsai SC, Lu CC, Lin CS, Wang PS. Antisteroidogenic actions of hydrogen peroxide on rat Leydig cells. J Cell Biochem. 2003 Dec 15;90(6):1276-86.

    14. Cronin MJ, Evans WS, Hewlett EL, Thorner MO. LH release is facilitated by agents that alter cyclic AMP generating system. Am J Physiol. 1984 Jan;246(1 Pt 1):E44-51.

    15. Andric SA, Janjic MM, Stojkov NJ, Kostic TS. Protein kinase G-mediated stimulation of basal Leydig cell steroidogenesis. Am J Physiol Endocrinol Metab. 2007 Nov;293(5):E1399-408. Epub 2007 Sep 11.

    16. Manna PR, Dyson MT, Stocco DM. Regulation of the steroidogenic acute regulatory protein gene expression: present and future perspectives. Mol Hum Reprod. 2009 Jun;15(6):321-33. Epub 2009 Mar 25. Review.
    17. Kostic TS, Stojkov NJ, Janjic MM, Maric D, Andric SA.The adaptive response of adult rat Leydig cells to repeated immobilization stress: the role of protein kinase A and steroidogenic acute regulatory protein. Stress. 2008;11(5):370-80.
    18. Kraemer WJ, Spiering BA, Volek JS, Ratamess NA, Sharman MJ, Rubin MR, French DN, Silvestre R, Hatfield DL, Van Heest JL, Vingren JL, Judelson DA, Deschenes MR, Maresh CM. Androgenic responses to resistance exercise: effects of feeding and L-carnitine. Med Sci Sports Exer., 2006 Jul: 38(7): 1288-96.
    19. Kraemer WJ, Volek JS, French DN, Rubin MR, Sharman MJ, Gómez AL, Ratamess NA, Newton RU, Jemiolo B, Craig BW, Häkkinen K. The effects of L-carnitine L-tartrate supplementation on hormonal responses to resistance exercise and recovery. J Strength Cond. Res. 2003 Aug: 17(3): 455-462.
    20. André C, Berthelot A, Robert JF, Thomassin M, Guillaume YC. Testimony of the correlation between DHEA and bioavailable testosterone using a biochromatographic concept: effect of two salts. J Pharm Biomed An., 2003 Dec 4: 33(5): 911-21.
    21. J. Raman, P. Schlegel AROMATASE INHIBITORS FOR MALE INFERTILITY The Journal of Urology, Volume 167, Issue 2, Pages 624-629.
    22. Burnett-Bowie SA, Roupenian KC, Dere ME, Lee H, Leder BZ. Effects of aromatase inhibition in hypogonadal older men: a randomized, double-blind, placebo-controlled trial. Clin Endocrinol (Oxf). 2008 Jun 25. [Epub ahead of print]
    23. Schubert K, Wehrberger K, Hobe G; Androsta-3,5-diene-7,17-dione: isolation from urine and formation from 7-keto-dehydro-epiandrosterone sulphate under various conditions of hydrolysis; Endocrinol Exp. 1971 Dec;5(4):205-10

    24. Numazawa M, Mutsumi A, Tachibana M, Hoshi K; Synthesis of androst-5-en-7-ones and androsta-3,5-dien-7-ones and their related 7-deoxy analogs as conformational and catalytic probes for the active site of aromatase; J Med Chem. 1994 Jul 8;37(14):2198-205

    25. Safarinejad MR, Safarinejad S. Efficacy of selenium and/or N-acetyl-cysteine for improving semen parameters in infertile men: a double-blind, placebo controlled, randomized study.J Urol. 2009 Feb;181(2):741-51. Epub 2008 Dec 16.
    26. Farombi EO, Ugwuezunmba MC, Ezenwadu TT, Oyeyemi MO, Ekor M.Tetracycline-induced reproductive toxicity in male rats: effects of vitamin C and N-acetylcysteine. Exp Toxicol Pathol. 2008 Jun;60(1):77-85. Epub 2008 Apr 11.
    27. Fernanda BM Priviero , Julio A Rojas-Moscoso, Alexandre S Silva, Edson Antunes and Angelina Zanesco Effect of L-carnitine supplementation on the sGC/cGMP pathway in vascular relaxing responses from exercised rats BMC Pharmacology 2009, 9(Suppl 1):P54doi:10.1186/1471-2210-9-S1-P54
    28. Cheng HJ, Chen T. [Clinical efficacy of combined L-carnitine and acetyl-L-carnitine on idiopathic asthenospermia] Zhonghua Nan Ke Xue. 2008 Feb;14(2):149-51.
    29. Rohle D, Wilborn C, Taylor L, Mulligan C, Kreider R, Willoughby D. Effects of eight weeks of an alleged aromatase inhibiting nutritional supplement 6-OXO (androst-4-ene-3,6,17-trione) on serum hormone profiles and clinical safety markers in resistance-trained, eugonadal males. J Int Soc Sports Nutr. 2007 Oct 19;4:13.
    30. Zhou X, Liu F, Zhai S. Effect of L-carnitine and/or L-acetyl-carnitine in nutrition treatment for male infertility: a systematic review. Asia Pac J Clin Nutr. 2007;16 Suppl 1:383-90.
    31. Willoughby DS, Wilborn C, Taylor L, Campbell W Eight weeks of aromatase inhibition using the nutritional supplement Novedex XT: effects in young, eugonadal men. Int J Sport Nutr Exerc Metab. 2007 Feb;17(1):92-108.
    32. Lee NP, Cheng CY. Nitric oxide and cyclic nucleotides: their roles in junction dynamics and spermatogenesis. Adv Exp Med Biol. 2008;636:172-85. Review.
    33. Ulcova-Gallova Z, Gruberova J, Vrzalova J, Bibkova K, Peknicova J, Micanova Z, Topolcan O. Sperm antibodies, intra-acrosomal sperm proteins, and cytokines in semen in men from infertile couples. Am J Reprod Immunol. 2009 Mar;61(3):236-45.
    34. Tang LF, Jiang H, Shang XJ, Zhao LM, Bai Q, Hong K, Liu DF, Liu JM, Yuan RP, Chen Q, Ma LL.[Seminal plasma levocarnitine significantly correlated with semen quality] Zhonghua Nan Ke Xue. 2008 Aug;14(8):704-8.
    35. WADA 2009 Guidelines
    36. Lab Corp® Guidelines: Test ID 104103.
    37. Thomas, J. and Nelson, J. Research Methods in Physical Activity. 132-208 Human Kinetics. 1996.
    Dirk Tanis, BA, MSci
    Chief Operating Officer, Applied Nutriceuticals
  28. Board Supporter
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    Quote Originally Posted by rms80 View Post
    Here is a decent read if you are interested
    Cool, thanks. Yeah that is much more realistic... and honest. If you read through it, it basically says that everyone's levels went up but because the study did not have a large enough sample size the results are not statistically significant:

    Six men between the ages of 18 and 49 yr (mean age, 32.5 ± 10.01) were studied.
    Due to the nature of pilot research (low number of subjects and only two variables measured)- the design of the study is a quasi-experimental One Group Pretest-Posttest Design.
    TT levels increased, but the increases were not enough to be deemed significant (p= 0. 1484) (37).
    In reference to changes in TT, while subjects did show a directional increase, but this increase was not significant (20).
    In layperson's terms this study says that results are inconclusive; they cannot say whether is works or does not work.
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    Scientifically Proven Natural Testosterone Boosters is TCF-1 from PP !
    done.

    and now its double sized at same price.
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    Quote Originally Posted by Nitrox View Post
    Normally I don't bother with this stuff because, well quite frankly, I think that 99.99% of it is goat caca. However the thread title got me interested in what sort of science is actually used.

    So for sh!ts and giggles I googled 'Testofen study' and came up with this: http://forum.bodybuilding.com/attach...hmentid=776901... (if there is a different one please let me know)

    From a science perspective this is a sorry excuse for a study and is basically just another form of 'infomercial.' I love this line:

    Free Testosterone levels in serum were estimated before and after 8-week consumption
    of TESTOFEN.


    IMO this wording basically says that there is no actual certainty in the product's effectiveness and gives the company a solid defense in the event of any legal challenge.

    The amount of deception in the supplement industry never ceases to amaze me.
    The industry is complex indeed. However, a person should be able to have test performed before, during and after. At least before and after. This would give definitives.
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    I thi k you misread the study. While TT had inconclusive results (mainly due to such a small increase), they did state a dramatic increase in FT, which is qll that appnut claimed, hense the name free test.
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    Quote Originally Posted by RAHHH View Post
    Scientifically Proven Natural Testosterone Boosters is TCF-1 from PP !
    done.

    and now its double sized at same price.
    Just saw that its on sale, and its scientifically proven. Summer Super Sale 2010
  

  
 

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