- 03-24-2010, 04:11 PM
Hey guys! I need some help understanding this study. It says 'addition of D-Asp to testicular tissue acetone powder induced a significant increase of aromatase activity.' The way I see it,increases aromatase of testosterone into a form of estrogen. This sounds negative, but all the supplement companies are starting to sell this stuff. Am I understanding the study wrong?
Endogenous testicular regulates gonadal aromatase activity in boar.
Lamanna C, Assisi L, Botte V, Di Fiore MM.
Department of Zoology, University of Naples 'Federico II', Naples, Italy.
(D-Asp), aromatase enzyme activity and the putative D-Asp involvement on aromatase induction have been studied in the testis of mature boars. The peroxidase-antiperoxidase and the indirect immunofluorescence methods, applied to cryostat and paraffin sections, were used to evaluate D-Asp and aromatase distributions. D-Asp level was dosed by an enzymatic method performed on boar testis extracts. Biochemical aromatase activity was determined by in vitro experiments carried out on testis extracts. D-Asp immunoreactivity was found in Leydig cells, and, to a lesser extent, in germ cells. Analogously, aromatase immunoreactivity was present in Leydig cells, but absent from seminiferous tubule elements. In vitro experiments showed that the addition of D-Asp to testicular tissue acetone powder induced a significant increase of aromatase activity, as assessed by testosterone conversion to 17beta-estradiol. Enzyme Km was not affected by D-Asp (about 25 nM in control and D-Asp added tests). These findings suggest that D-Asp could be involved in the local regulation of aromatase in boar Leydig cells and intervenes in this organ's production of estrogens.
PMID: 16610240 [PubMed - indexed for MEDLINE]
- 03-24-2010, 04:28 PM
It looks that the consensus in all the studies about D-Asp is that it can do both things: increase test AND increase estrogens
Summary. Mammalian testis contains D-aspartic acid (D-Asp), which enhances testosterone production. D-Asp, on other hand, also stimulates 17β-estradiol synthesis in the ovary of some lower vertebrates. We studied boar testis in order to determine if D-Asp intervenes in 17β-estradiol synthesis in the testis of those mammals which produce significant amounts of estrogens as well as testosterone. The boar testis contains D-Asp (40 ± 3.6 nmol/g tissue) which, according to immunohistological techniques, is localized mainly in Leydig cells, and, to a lesser extent, in sustentacular (Sertoli), peritubular and some germ cells. The enzyme P450aromatase is present in Leydig cells and few germ cells. In vitro experiments showed that the addition of D-Asp to testicular tissue extracts induced a significant increase of aromatase activity, as evaluated by testosterone conversion into 17β-estradiol. The enzyme’s Km was not affected by D-Asp (about 25 nM in both control and D-Asp added tests). On the basis of these results we suggest that, as in the ovary, D-Asp is involved in the local control of aromatase activity of boar testis and, therefore, it intervenes in the 17β-estradiol production. In the testis, the D-Asp targets are presumably the Leydig cells, which having also a nuclear estrogen receptor are, in turn, one of the putative targets of the 17β-estradiol that they produce (autocrine effect).
If that is true (and i think that is true, because almost every study say the same: D-Asp increase test AND estrogens), then i don't understand all the recent hype with D-Asp
- 03-24-2010, 07:55 PM
There is going to be an elevation in E with an elevation of T, just depends on how much it raises the E. Our bodies generally aim to maintain a certain ratio of T:E, so some elevation in E isn't always a bad thing
03-24-2010, 08:07 PM
03-24-2010, 08:11 PM
03-24-2010, 08:20 PM
03-24-2010, 08:54 PM
03-25-2010, 10:38 AM
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03-26-2010, 11:30 AM
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