Phaseolamin - alpha-amylase inhibitor

DAdams91982

DAdams91982

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Basically I want to know if anyone has tried Phaseolamin (The active in White Kidney Bean Extract) to block starchy carbs with success? If this works to inhibit alpha-amylase then it would make carb dieting slightly easier. Of course not relying on it, but having that french fry every once in a while would help curb some heavy cravings.

Determination of alpha-amylase inhibitor activity of phaseolamin from kidney bean (Phaseolus vulgaris) in dietary supplements by HPAEC-PAD.

Mosca M, Boniglia C, Carratù B, Giammarioli S, Nera V, Sanzini E.
National Center for Food Quality and Risk Assessment-Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy.
Some dietary supplements, so-called 'starch-blockers', used to control overweight, are based on the protein concentrate of the kidney bean, known to contain high levels of the alpha-amylase inhibitor phaseolamin, which may hinder the digestion of complex carbohydrates, thereby promoting or supporting weight loss. Currently, methods to determine the levels of alpha-amylase inhibitor are based on the measurement of alpha-amylase activity using colorimetric methods that cannot be applied to dietary supplements because they are complex mixtures of different ingredients that may interfere with the measurement. The aim of this study was to develop an alternative method to determine the level of phaseolamin in dietary supplements, using high-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) to measure the amount of maltose resulting from the action of the enzyme porcine alpha-amylase on soluble starch in the presence and absence of the inhibitor. The assay described proved sensitive and accurate for use with both dietary supplements and raw materials.
 

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