This will be my first time running these experiments, I'm going to be making a short estered mixture (which is usually found at 100mg/ml) and capsules. I have a lot of (stupid) questions and I'm making more of it than I need to but I would prefer to ask and understand as much as possible going in rather than make a mistake and ruin my gear or worse.
Starting off with 50ml, the mixture I want is something like: 50/50 ethyl oleate/USP oil with 125 or preferably 150 mg/ml of substance. I was thinking 2% BA and 10-12% BB, does this sound like the best possible combo for painless, sterile, and no suspension crashing?
Cottonseed oil is available, but it seems like some people say its advantageous to get grapeseed, any opinions on this?
Some people have said ethyl oleate should be stored in amber vials because it is light sensitive. How will you know if your suspension has crashed or gone bad if its in an amber vial? What kind of expiration date might one expect, is it the oil that degrades over time, the ethyl oleate, or the substance being suspended? Whats the best method for storing powder for maximum life (possibly sealed in the freezer?).
sterilize area with alcohol pads
place powder, ba, bb, oil in flask
What kind of flask do most people use for brewing/mixing, an Erlenmeyer or just a simple 250ml glass beaker? Do you have to heat the flask to sterilize before brewing?
Is heating the mixture necessary or is stirring with a glass stirrer till powder is gone then filtering through a .45 micron whatman enough?
Is it best to filter the whole solution into 1 large 50 or 100ml sealed sterile vial, and let it sit to make sure it doesn't crash, then break it up into smaller 20ml vials with a 20ml syringe?
Anyways, thats all I have right now