baking temperature

[daemonium]

will bake a solution at 160º for 4 hours destroy the hormone?
what do you think?

 

[Neuromancer]

Dont bake...there really is no need for it.

Just filter with a .22 filter and be done with it. The largest tempature change you can cause without damaging the hormone is not enough to kill the bacteria that could possibly exist in your oil.

Here are some good posts

http://www.sculptedbyiron.com/forum/showthread.php?t=13552

If I can find a lab report that was posted somewhere about BA killing bacteria in solution I will post it...cant find it for the life of me right now.

 

[DR.D]

If you do bake, 1 time at 160 is less destructive than 2 times at 120. I rarely bake though.

 

[daemonium]

so for NOT destroying the hormone is prefered to bake 2 times @ 135ºC four one hour? It woun't destroy almost any organisms, but at least will be another sterilization step, rather than only the removal one by the filter.

Everyone shows study where baking can or cannot destroy bacteria, but i can't see any of them showing it will destroy some types of hormones althoug it is certain to happen. and wich temp will result in destruction for wich hormones.

 

[DR.D]

Some people think it will kill more bugs to bake it twice and use 120' thinking it won't hurt the hormone. I'm saying, it's less destructive just cooking it once at 160' for 2hrs that heating it twice at 120' for 2hs/each. If you just filter it with a 0.2mic with at least 2% BA in the mix (5% is better IMO) then let it sit a day or two before you use it, it will be fine with no baking. Especially if you use a sterile filter.

 

[Neuromancer]

Well what I was looking for was in the thread I already posted :think:

but here it is again.

 

[daemonium]

Some people think it will kill more bugs to bake it twice and use 120' thinking it won't hurt the hormone. I'm saying, it's less destructive just cooking it once at 160' for 2hrs that heating it twice at 120' for 2hs/each. If you just filter it with a 0.2mic with at least 2% BA in the mix (5% is better IMO) then let it sit a day or two before you use it, it will be fine with no baking. Especially if you use a sterile filter.

The thing is that the only reasonable method that we can use at home for sterilize, dry heat, in proper use will destroy it or almost destroy it.
Well filtering (and the more aseptic techniques the better in the process) is really the only way to go, but really the endotoxins and virus will go trought.. and we will inject them IM. BA will help to maintain the stolution minimally bacterial free, and it will kill some virus that have lipid membranes, but all others will remain there...
I think i'm gonna try the 0,1um nylon filters, at least it will filter the doble of the 0,2um... although i expect a lot of time dedicated to handwork!

 

[DR.D]

Well what I was looking for was in the thread I already posted :think:

but here it is again.

I couldn't link, guess i need to be a member

 

[DR.D]

The thing is that the only reasonable method that we can use at home for sterilize, dry heat, in proper use will destroy it or almost destroy it.
Well filtering (and the more aseptic techniques the better in the process) is really the only way to go, but really the endotoxins and virus will go trought.. and we will inject them IM. BA will help to maintain the stolution minimally bacterial free, and it will kill some virus that have lipid membranes, but all others will remain there...
I think i'm gonna try the 0,1um nylon filters, at least it will filter the doble of the 0,2um... although i expect a lot of time dedicated to handwork!

I've never seen a 0.1 micron nylon filter, but I suggest PTFE/GMF if your using co-solvents. I agree w/ you about the sterilization, I just don't heat. I seem to get away with it just fine usually. BA is not so effective on non-vegetative spores and viruses, but they are going right through even a 0.1 filter anyway.

 

[Neuromancer]

Even if some bacteria can make it through to your muscle, the human body is much more keen on ridding itself of bacteria. Its really the foreign particles that pose the greatest threat imo.

Won't let me cut and paste the chart Doc.

 

[daemonium]

I've never seen a 0.1 micron nylon filter, but I suggest PTFE/GMF if your using co-solvents. I agree w/ you about the sterilization, I just don't heat. I seem to get away with it just fine usually. BA is not so effective on non-vegetative spores and viruses, but they are going right through even a 0.1 filter anyway.

There are i dunno if all companys do them, but here is one http://www.osmolabstore.com/OsmoLabPage.dll?BuildPage&1&1&1052 ! Some of the nylon are used in pharmaceutic companies, but what do you call "co-solvents"? It's a stupid question, but i just can only think in the general solvents, alcohools or condensation polymers like PEG..or? Yup PTFE/GMF are the usually more used, but they only have minimum size 0,2!

Well, after all what matter is that we don't get sick/abcess with this...
And neuromancer, yeah i agrree with you, strange particules may cause a likely abcess! But some endotoxins would be a hell of a **** if they enter in body, but we can't do nothing about it since it is almost a matter of lucky or not with the preparation solvents and the powder!

At home is all we've got... we have to use it.

 

[DR.D]

There are i dunno if all companys do them, but here is one http://www.osmolabstore.com/OsmoLabPage.dll?BuildPage&1&1&1052 ! Some of the nylon are used in pharmaceutic companies, but what do you call "co-solvents"? It's a stupid question, but i just can only think in the general solvents, alcohools or condensation polymers like PEG..or? Yup PTFE/GMF are the usually more used, but they only have minimum size 0,2!

Right, that's what I meant by C/C's, some can degrade nylon. That's intresting about the GE filters. I was unaware that 0.1mic Nylons were even available. Thanks for the info!

 

[daemonium]

you were a good help too! thks

 

[sikdogg]

I always bake... but i bake at 250deg F. for 15 minutes, cool in the fridge for 15 mins, and repeat the cycle 2-3 times.

In the past, Chemo (the previous board owner) stated something to the effect that the length of time at temp isn't what kills the bacteria but the rapid change in temp. I've always baked like this and swear by my results. All of my buddies that have tried my homebrew gear have thrown away all of their vet gear crap and swear by my stuff.

 

[daemonium]

I think i've found a solution for me, found a guy that can provide me gamma radiation very cheap!
so i just need to filter and send them the package with the vials closed, he sterilizes it and send me back in!

 

[DR.D]

I think i've found a solution for me, found a guy that can provide me gamma radiation very cheap!
so i just need to filter and send them the package with the vials closed, he sterilizes it and send me back in!

It's a great option, just be careful with 4-ene-3-one structures. They are usually quite photo sensitive and isomerize easily.

 

[AgnosticFront]

Dont know much about this so im gonna sound stupid. Your saying heat destroys the hormone... WHat is the point of baking? Many bacteria thrive at a temp 120-160! I would think autoclave would be the only real viable method for home sterilization? How much dammage would pressure sterilizing do to the hormones?

 

[daemonium]

It's a great option, just be careful with 4-ene-3-one structures. They are usually quite photo sensitive and isomerize easily.

I'll keep that in mind,
That should include wich substances?
are your refering as like nandrolone decanoate, 19-Nor-4-androstene-3-one,17b-ol?
that would be a not good new, since test E, test bold un, tren E as almost most of injectables have a similar structure!
Dr.D so you don't think it's a good option for injectables? since if I undestood right the structures, most of injectables will isometrize with gamma radiation!

 

[DR.D]

I'll keep that in mind,
That should include wich substances?
are your refering as like nandrolone decanoate, 19-Nor-4-androstene-3-one,17b-ol?
that would be a not good new, since test E, test bold un, tren E as almost most of injectables have a similar structure!
Dr.D so you don't think it's a good option for injectables? since if I undestood right the structures, most of injectables will isometrize with gamma radiation!

Assuming he's using a Cs or Sr source, you may be OK. Non-ionizing is not a safe bet, because UV is a real no no, but clean gamma sources may be alright. You'll know by the results. Beta emittion will isomerize too, of course.

 

[daemonium]

It will use 270 kCi of Cobalt-60 radiation! (60Co)i don't have more specifications for now..
so i'm not sure about it, but theorically it should be ok for most of phamaceutical products.

 

[DR.D]

It will use 270 kCi of Cobalt-60 radiation! (60Co)i don't have more specifications for now..
so i'm not sure about it, but theorically it should be ok for most of phamaceutical products.

I'm away from home right now, and been outta school too long to remember, but I think Co60 emits a low energy beta too. As long as it's geometry is such that it's exposed only to the gamma, it's worth a shot. I still think A-ring saturated comounds would be best with this, let me know how it works. That's a big source too! What's the protocol for the exposure? How many RAD's or mR? When I get home, I'll find the info and let you know what the real deal is for the comounds you mentioned.

 

[AgnosticFront]

OK..... So again.... Is it a bad idea to pressure sterilize injectables or a good idea?

 

[DR.D]

OK..... So again.... Is it a bad idea to pressure sterilize injectables or a good idea?

I doubt it would hurt, but I've never tried it.

 

[daemonium]

I'm away from home right now, and been outta school too long to remember, but I think Co60 emits a low energy beta too. As long as it's geometry is such that it's exposed only to the gamma, it's worth a shot. I still think A-ring saturated comounds would be best with this, let me know how it works. That's a big source too! What's the protocol for the exposure? How many RAD's or mR? When I get home, I'll find the info and let you know what the real deal is for the comounds you mentioned.

I'll try to know more technical specifications in the following days, and i'll post here since now i can't get in touch with the guy!
Thnks for the big help Dr.D!

 

[DR.D]

I'll try to know more technical specifications in the following days, and i'll post here since now i can't get in touch with the guy!
Thnks for the big help Dr.D!

OK, it looks like your safe. UV radiation of 300nm or less is the main culprit. So avoid exposure to direct sunlight. However, in one of the ref's I'm looking at, it states that low-velocity electrons (beta's), high frequency electrons, alternating current, x-rays, and cathode rays all can result in oxidative rearrangements. Usually the B ring is opened or the 4-ene is reduced or converted to the delta 5 structure. Gamma radiation is really the same as an x-ray, only difference is that it originates in the nucleus. Still, I say go for it. It's an excellent sterilization technique and if something get's degraded, it'll probably let you know by changing colors or precipitating out of solution.

 

[daemonium]

I'll go for a try soon as I can with some test E since it's cheap and let you know! I'll try to take some pics.. though it will take a bit because i've only planned to homebrew them in september, if i can i'll try to do than earlier!
I didn't knew that UV would affect the hormone, so germicidal uv lamps (254nm) are out of question with the hormones near by!
thks D, you have been a excelent help!

 

[DR.D]

thks D, you have been a excelent help!

No problem. Please let me know how it turns out once you get around to it.

 

[WATERLOGGED]

DR. i am always amazed you are one smart bro !i dont think there is a single topic that you dont know about....man i wish i knew that much....lol :box:

 

[DR.D]

DR. i am always amazed you are one smart bro !i dont think there is a single topic that you dont know about....man i wish i knew that much....lol :box:

Thank you for the compliment, Waterlogged. It is most humbling. You should not be so impressed though, after all, my major was nuclear physics so I better know the answer to this one! BTW, drop me a PM and let me know how the HRT is going.

 

[daemonium]

D. can you find out what are the best specifications for radiosterilization with co60 in these injectable coumpounds?
I talked to a friend that said me that they usually work with pharmaceutical products (possibly not all) and they can regulate the exposure! I've send a mail to the guy and later or tomorrow i may have the specifications they usually use in pharmaceutical prods! If you know anything about it on this products, I can ask them the regulate the emitter for that precise specifications!

 

[DR.D]

D. can you find out what are the best specifications for radiosterilization with co60 in these injectable coumpounds?
I talked to a friend that said me that they usually work with pharmaceutical products (possibly not all) and they can regulate the exposure! I've send a mail to the guy and later or tomorrow i may have the specifications they usually use in pharmaceutical prods! If you know anything about it on this products, I can ask them the regulate the emitter for that precise specifications!

Sure thing. I'll do some research and get back with you tomorrow. I have some good, relevant data on this at work.

 

[daemonium]

very nice! If this go well I don't have to hurry mutch about the sterility in my kitchen!

 

[Old Guy]

http://www.sculptedbyiron.com/forum/showthread.php?t=13552

This thread was started by SV-1 at SBI Board. It is a pretty long read with several links.
In short it says:
Why I don't autoclave/bake my gear. - By SV-1

I've seen a lot of info on the board lately about how using an autoclave or pressure cooker will sterilize oil based AAS, well after a good bit of research everything I've been able to find states the exact opposite.


To start here is a quote by Justin, a moderator at UK Muscle:
Quote:
Clearing up some misconceptions

Heat sterilisation of oil will not be successful by using 250F.
250F is the heat used by a certain class of autoclave. The autoclave uses pressure also to achieve wet sterilisation, this method will work for aqueous solutions but will NOT sterilise oil. If heat is the chosen method to sterilise oil, then you must sterilise by dry heat methods, i.e. 150-170C (302 - 338F) for 1-4 hrs, (type and volume depending) which can be detrimental to certain hormone preparations.
Divert your attentions to using clean practises, filtering with a 0.22um membrane filter and incorporating a Bacteriostatic agent (BA).

 

[AgnosticFront]

thanks for the answer. :thumbsup:

 

[DR.D]

D. can you find out what are the best specifications for radiosterilization with co60 in these injectable coumpounds?

The standard pharmaceutical expose for photosterilization is not less than 5 kGy and not more than 30 kGy (+/- 10%) at no closer than 1 ft away from a Co60 source. A Gray(Gy) is an international unit equivalent to 100 RAD's or 100 Roentgens for radiation absorbed dose or actual deposited dose. Co60 emits 2 high energy photons that are capable of indirect ionization, so I suggest the lowest exposure initially to assess detrimental effects on the preparation.

 

[DR.D]

thanks for the answer. :thumbsup:

I heard from a guy who does this and he thinks it actually helps prevent oxidation when heat sterilizing. So it looks like pressure+heat sterilization may be a legit method. :thumbsup:

 

[daemonium]

The standard pharmaceutical expose for photosterilization is not less than 5 kGy and not more than 30 kGy (+/- 10%) at no closer than 1 ft away from a Co60 source. A Gray(Gy) is an international unit equivalent to 100 RAD's or 100 Roentgens for radiation absorbed dose or actual deposited dose. Co60 emits 2 high energy photons that are capable of indirect ionization, so I suggest the lowest exposure initially to assess detrimental effects on the preparation.

nice info.
I contacted the guy, and he said usually they expose the pharmaceuticals products to 12kGy, but also said that they should know what's the substance to be sterilized so they can do a proper sterilization.. they are starting to ask too many questions.. I'll have to find a way to go around this, since i didn't want to tell them what's really in there. any idea D?
for the "experience of radiosterilization effects on some unknown yet samples of my pharmacy university work" (that's the words i used for lmao)
now or i just tell them that it's aas there, and they may tell me to get lost or i can try to say there's something else there...

 

[DR.D]

nice info.
I contacted the guy, and he said usually they expose the pharmaceuticals products to 12kGy, but also said that they should know what's the substance to be sterilized so they can do a proper sterilization.. they are starting to ask too many questions.. I'll have to find a way to go around this, since i didn't want to tell them what's really in there. any idea D?
for the "experience of radiosterilization effects on some unknown yet samples of my pharmacy university work" (that's the words i used for lmao)
now or i just tell them that it's aas there, and they may tell me to get lost or i can try to say there's something else there...

You could say it's an unknown oil composition. Or you could admit it was test and preped for you by a compounding pharmacy. Slap a generic Word label on it (like they use) includes a dr's name, amount, date, instructions, concentration, pharm name, etc.. I don't know. I'd probably just send it as unknown and and risk wasting a small batch of it to see if it worked.