FROM SPYDIE AT BOLEX
Step 1.) MAKING BACTERIOSTATIC WATER.
1. Put distilled water into a pan and bring to a boil for 2 minutes.
2. Now add ice to bring water to a cold temp as quickly as possible.
3. Add 1% Benzyl Alcohol and .25% PolySorbate 80 to sterile container **percentages need to be in proper ratio to total volume of liquid**
4. Make sure all ice is melted, and then filter water into large sterile container where we added the BA and PS80 (this is where we will store our “bulk� bacteriostatic water.)
5. Now swirl the mixture so that it is totally absorbed, and now our bacteriostatic water is finished.
Guys, I've been working my butt off to perfect this and I finally got it. Here is a formula for Test no ester (water based suspention) at 75mg/ml that will pass through a 30ga slin pin with no clogging. ENJOY!!!
TNE suspention 75mg/ml
Supplies needed
1.5 Grams TNE powder
2 .22um whatman filter
1 10ml vial
1 20ml vial
2 18ga pins with 3ml plungers
1 5 ml plunger
1 10ml plunger
2 ml Benzyl Benzoate (10%)
.4 ml Benzyl Alcohol (2%)
.6 ml Polysorbate 80 (3%)***Trust me on this***
15.875 ml Distilled water
1. Add your BB and PS80 to your beaker (10ml vial) with your TNE powder and heat until it is in liquid form.
2. reduce heat enough to keep it liquid (med low temp on a hot plate) ***note: you must keep it on heat so it does not crash while filtering into the distilled water***
3 Put .4 ml BA and 15.875 ml distilled water in your 20ml vile.
4. Most of you will prefer to filter the BB/PS80/TNE mix which it great, but I prefer just to use a glass dropper to slowly drop it into the water 1ml at a time (BECAUSE GLASS WILL NOT MELT IN YOUR HAND) However, if you want to filter the concentrate use a .45um whatman filter and only do 1 ml at a time.
5. slowly add your BB/PS80/TNE mixture to your water 1 ml at a time, and after every ml you add put the stopper on the vile and shake vigerously. REMEMBER ONE ML AT A TIME. **THIS IS VERY IMPORTANT**
by the time you're done it should be milky white and perfect, with this method it won't clump or seperate to the bottom like it did with PEG. The TNE will stay milky and completely seperated and will go through a 30ga by 1/2" slin pin like pure water with no clogging and no pain at all!
Trust me you guys will love this!
