Say goodbye to your "lil' buddy" for a while. He'll be on a leave of absence for 6 weeks or so... especially if you dose as high as 2.5mg. I say this from experience. Also, myself and others have reported feeling absolutely miserable on letro. Having around 97% of your estrogen eliminated feels pretty crappy. Definitely took care of my gyno though. (And yes, we're talking hard, painful, sensitive lumps.)
As far as your measurement goes, yes... 1ml should equal 2.5mg. I'm not sure it makes a difference what time of day you take it, although I dosed at night in an unsuccessful attempt to avoid feeling as rotten during the day.
Honestly, for "puffy and soft," I would only dose as high as 1mg at the peak of the cycle... if I took it at all. Letro's some strong stuff. Just my $0.02.