Filtering Suspect UGL Gear
- 07-22-2010, 10:51 PM
Filtering Suspect UGL Gear
I have 30 ml of Sustanon from a local UGL source. After my first pin (quad) I came down with an infection in the area most likely cellulitis. 3 days of antibiotics and the pain and swelling have subsided. At this point I can only speculate as to whether the gear is contaminated with bacteria, or if bacteria entered through the injection site post-shot.
I have researched a bit in regards to .2um whatman filters. Are these used mainly for filtering out particulates or can they be used on gear that is most likely contaminated with bacteria? The gear is dosed properly and many have made solid gains using this local UGL, but it seems that as luck should have it, these are tainted.
I will have extra antibiotics on hand should I proceed with using the whatman/bake method in an attempt to re-sterilize my gear (if it was even sterile in the first place). Am I playing Russian roulette with another possible infection, or is this method sufficient?
- 07-23-2010, 01:28 AM
07-23-2010, 08:34 AM
If there is bacteria in it, you will have to add some benzyl alcohol, but the source would have already used BA when making it. You could add more, but theres no way to tell how much to add. And if the source already has used a high amount of BA, adding more is going to make it even more painful. Adding some BA and filtering may help, but its not a guarantee.
Not knowing whats exactly wrong with your gear, essentially you are taking the risk of having another infetion regardless of what you do.
07-23-2010, 11:19 AM
I would not even think twice about refiltering my gear. Personally tho, if i got an infection from it, i'd chuck it and forgot about that source but like you said, its hard to determine who the culprit is.
Hi-Tech Pharmaceuticals Representative
07-23-2010, 01:01 PM
Throwing it away is the safest bet. Talk to your source about it as well.
No need to bake your gear as you can't get it to a high enough temperature to make it sterile without scorching your gear. That's what the membrane filters are for.
If you're not going to throw it away and you are going to try and save it, here's what I recommend.
1. Use very sterile practices when doing this. Wipe everything down with Isopropyl alcohol. work area, stirring rods, glasswear, etc.
2. Get new pre-strerilized vials.
3. Filter through a .45 sterile millipore syringe filter.
4. Filter into new vials through a .22 or .20 millipore syringe filter.
5. If you want to add more BA only add a little, maybe 1% as it will hurt bad if you have too much.
Remember, the BA only helps keep the gear clean once its already clean. It does NOT clean the gear for you.
07-23-2010, 06:30 PM
Thanks for the input guys. I know the best idea is to toss this crap, but unfortunately giving re-sterilizing a shot with some extra antibiotics on hand is the most economical choice for me right now.
I have done the research regarding which filters to use (thanks for the step by step process rhodesman). Does anyone have a link or can explain exactly what I will need to filter 30 ml of gear? I am looking for how many filters/pins, etc. Also, is there a certain syringe/pin size that works best?
As for the source, I am going through a buddy who goes to the local guy directly. The most frustrating part of this whole deal is the fact he got burned too by an infection on his glute, yet he refuses to even tell the source. You would think as someone who gives him quite a bit of business he would be comfortable in relaying this. Regardless, from this point on its human grade only, to hell with these infections!
08-01-2010, 03:14 PM
Just my advice each time I use a new vial, cuz everyonr can make mistakes, I inj only like a tiny tiny amount and wait 24hours if it is infected the small amount should not cause much damage when I say small I mean it like 1/8 of 1cc
08-01-2010, 03:16 PM
08-05-2010, 08:52 AM
I took clindamycin for the infection.
Here is the update: I re-filtered the gear last saturday and added 1% BA. I filtered with a .45 first, then a .22. I also heated the bottle gear on the stove for 20 mins at medium heat as an additional measure. This turned the oil form a light yellow to a amber color. I did an injection on Saturday and woke up Monday am with the site sore. Not hot or red, but a bit swollen and sore when bending my leg or massaging the area. The pain is no where near that of when I had a full blown infection, but now going on day 4 of pain I am beginning to worry.
From my research some guys report that injections which include prop can be painful, but for how long? Could this be from the added BA? Again my first pin was Saturday night with the soreness and a bit of swelling beginning Monday am.
I do have an additional 7 days worth of anti's, which I have on hand incase my science project did not work as planned. Any insight would be helpful.
08-05-2010, 09:37 AM
honestly I wouldn't have filtered or added BA, just baked it at 200 degrees for a half hour. That would kill any bacteria. The pain from prop or excess BA could easily last 3 days, 4 isn't totally unlikely. Part of the pain is recrystalization, part of it is dehydration of the local cells due to the BA.
08-05-2010, 09:51 AM
I had my gf feel both my quads to see if she thought one was warmer than the other, this was last night and she said she noticed nothing. She did mention the muscle felt "tense" on the quad which I had pinned. I am just surprised as to how long this has lasted with no change for the better.
Any insight as to why the gear became darker when heating?
08-05-2010, 10:08 AM
it could cause some hardening sure. Sometimes the color does change when baking gear as well, but so long as you are keeping it below a certain temp, its ok. I can't recall what the temp is, but 200 is ok for everything
08-05-2010, 10:24 AM
08-05-2010, 10:25 AM
08-05-2010, 10:28 AM
08-05-2010, 10:28 AM
08-05-2010, 10:38 AM
DRY HEAT STERILIZATION OF PARENTERAL OIL VEHICLES.
T Kupiec1 , R Ahmad2 , P Matthews3 , L V Allen, Jr.4
1Analytical Research Laboratories, Edmond, OK, 2University of Central Oklahoma, Edmond, OK, 3Analytical Research Laboratories, Edmond, OK, 4Midwest Institute of Research and Technology, Edmond, OK,
The purpose of this study was to evaluate the effect of temperature and time on the dry heat sterilization conditions of three different parenteral oil vehicles. Methods. Three different oils, cottonseed oil, peanut oil, and sesame seed oil were each spiked with Bacillus subtillus spores. The inoculated oils were exposed to dry heat at 4 different temperatures (150ºC, 160ºC, 170ºC, and 180ºC) for three time intervals (1, 1.5 and 2 hours). Following inoculation and dry heat sterilization, samples were placed in a sterile laminar flow hood and processed according to <71> Sterility Tests of the USP XXIII using thioglycolate broth and fluid D. The specimens were then placed into the incubator at 30ºC for 3, 5 and 7 days and observed for bacterial growth. The above variables were performed in triplicate. Positive and negative controls were run along with each variable and group for quality control. Results. Cottonseed oil, peanut oil, and sesame seed oil were found to be free of Bacillus subtillus following dry heat sterilization at all four temperatures for 1, 1.5 and 2 hours at 3, 5 and 7 days. The positive controls were positive for observed growth and the negative controls had no observed bacterial growth. Conclusions. Dry heat sterilization of parenteral oils at 150ºC for one hour was sufficient time and temperature. However the authors recommend dry heat sterilization at 160ºC for one hour after the oil has reached the desired temperature. These studies were partially funded by the Professional Compounding Centers of America,Inc
08-05-2010, 10:39 AM
So that shows granted at 150c for an hour, that it acheived sterilization. that was straight oil, innoculated with bacteria. Assumption is that with the BA already in the solution that a lower temperature and/or time will likely work.
08-05-2010, 10:42 AM
adding some more fun details
"According to the Wilderness Medical Society, water temperatures above 160° F (70° C) kill all pathogens within 30 minutes and above 185° F (85° C) within a few minutes. So in the time it takes for the water to reach the boiling point (212° F or 100° C) from 160° F (70° C), all pathogens will be killed, even at high altitude.""What is not well known is that contaminated water can be pasteurized at temperatures well below boiling, as can milk, which is commonly pasteurized at 71°C (160°F)...".
08-05-2010, 10:46 AM
I have 2 previous cycles under my belt. 12 months ago I ran Test E (human grade, I got lucky) with little to no pain during or after injections. My 2nd cycle, which was about 6 months ago, was a 12 week run to BD Cypionate (i think it was garbage) and anavar. I have never had so much pain after a shot!
08-05-2010, 10:47 AM
EasyJL; Going by the lowest temp. you quoted...150C equates to 302F. That's over 100 degrees difference from the 200 you said earlier.
I would also like to note that this temp would literally "cook" the oil, but that's fine in the study you provided as they only wanted to show what temp would kill the bacteria. Its a common misconception that oil can be heated enough to kill bacteria without ruining the gear. Low temps are are used to help speed the process of making the hormone soluble. Filters are what generally remove bacteria, viruses, mycoplasms, etc...
08-05-2010, 10:54 AM
08-05-2010, 10:58 AM
08-05-2010, 11:02 AM
08-05-2010, 11:04 AM
Pasteurization is a method used to kill SOME of the bacteria in milk, alcoholic beverages, etc... It does so by bringing the temperature to a certain point to where it kills only a percentage of the resident bacteria but not high enough to denature the proteins in the beverage. (Curdled milk would be hard to sell, lol). This is why milk has a sell by date. After that date, the bacteria count will rise to a point where the milk will smell spoiled. It wont be harmful to you yet, but who wants milk that smells bad?
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