[acshadow]

stuff looks really interesting. DGAT inhibition, NO production, antioxidant...looks pretty cool

Entrez PubMed

Nitric oxide (NO) has been proved to be a potent vasodilator that played an important role in regulating vascular tones. Tanshinone, one of the active components of Radix Salvia miltiorrhiza, was used widely in clinics in China for treating cardiovascular diseases. The objective of this study was to sensitively and specifically investigate the effects of tanshinone IIA, one important pharmacological constituent of tanshinone, on the release of NO from human vascular endothelial cells (HVECs) by fluorescence imaging with an excellent fluorescent probe 1,3,5,7-tetramethyl-2,6-dicarbethoxy-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacence (TMDCDABODIPY). After cells were incubated with tanshinone IIA, TMDCDABODIPY was employed to label NO. Following the tagging, real-time imaging of NO release from the cells was performed with inverted fluorescence microscope. The results of the experiments showed that tanshinone IIA could induce NO production significantly enhanced in HVECs. The activation of NO by tanshinone IIA may be employed therapeutically in modulating NO production in HVECs.

Entrez PubMed

The inhibitory activity of tanshinones from Salvia miltiorrhiza was tested on rat liver diacylglycerol acyltransferase (DGAT). Cryptotanshinone (1) and 15,16-dihydrotanshinone I (3) exhibited potent DGAT inhibitory activities dose-dependently with IC50 values of 10.5 microg/ml and 11.1 microg/ml. However, tanshinone IIA (2) and tanshinone I (4) showed very weak inhibition (IC50 value: > 250 microg/ml). A dihydrofuran moiety was seemed to be responsible for the stronger inhibitory activity.

Entrez PubMed
Tanshinone IIA is the major antioxidant component in the traditional Chinese medicine Salvia miltiorrhiza. Transcription factor nuclear-factor-E2-related factor (Nrf2) regulates a battery of antioxidant response element (ARE)-regulated genes. The aim of this study was to determine the effect of tanshinone IIA on Nrf2 activation and intracellular redox status in human aortic smooth muscle cells. Tanshinone IIA potentiated tumor necrosis factor alpha (TNF-alpha)-mediated nuclear accumulation of Nrf2 and expression of ARE-related genes, while it reversed TNF-alpha-induced down-regulation of intracellular glutathione (GSH), NADPH and glucose 6-phosphate dehydrogenase (G6PDH) levels. Specific silence of Nrf2 by siRNA down-regulated tanshinone IIA-induced Nrf2 activation and increased of intracellular GSH, NADPH and G6PDH levels. Tanshinone IIA-induced Nrf2 activation was association with activation of ERK and PKB, which was prevented by treatment with PD098059 or wortmannin. Tanshinone IIA attenuated TNF-alpha, angiotensin II, H(2)O(2)-mediated reactive oxygen species (ROS) production. These results demonstrated that tanshinone IIA-induced Nrf2 activation is the major regulatory pathway of cytoprotective gene expression against oxidative stress via ERK and PKB signaling pathways.

[TheMyth]

WOW that would be ausome, put me down for a Kilo! That is some good stuff. That with TTA, and green tea would stop fat from being put on period! O wait, isn't that DCP!!!??? Get some DCP while Dsade works on getting this. Get on it monkey man!

[knightowl]

Yes please I already requested that .I am glad others are interested too...

[dsade]

So you are saying this is something you are interested in in bulk? It is FAR from cheap.

[TheMyth]

I would be more than interested! Make it happy my lab monkey, and let me test it out, or just put it up to buy! Now get on it, don't make me pull out the whip! O I have an ausome Idea, Bulk Phenogen! And Bulk Leptigen!!!

[acshadow]

1fast400 used to have this

Salvia Miltiorrhiza Powder (40% tanshinones)

100 grams

$19.99

[dsade]

Originally Posted by acshadow

1fast400 used to have this

Salvia Miltiorrhiza Powder (40% tanshinones)

100 grams

$19.99

I am the one that ordered that material. Prices have gone up since then, but we can probably do 75 grams for the same price.

[acshadow]

whats the dosing recommendation?

[dsade]

Originally Posted by acshadow

whats the dosing recommendation?

250-500mg, 2-3 times per day would inhibit DGAT very nicely, preventing triacylglycerides from being reformed in the liver.

[stxnas]

75gm would last 50 days if taking 500mg three times a day. That sounds pretty reasonable to me for ~ $19.99

[acshadow]

Originally Posted by stxnas

75gm would last 50 days if taking 500mg three times a day. That sounds pretty reasonable to me for ~ $19.99

this man speaks the truth

[dsade]

Ok..Done. Material should arrive in a week or so.

[TheMyth]

holy shiate muslim! You work quick Dsade! You must be the ladies man in bed! Pre-orders now!

[acshadow]

wow...

[mrmoose63]

Things to remember with Salvia Miltiorrhiza:

1.It takes uber nasty and crystal light won't cover it up, so it needs to be capped.

2. It needed to be capped extremely quickly as it gets very sticky and becomes very hard to cap when not in sealed container.

3. Use with bulk sesathin for an ultimate fat loss stack.

[Sir Savage]

Originally Posted by TheMyth

I would be more than interested! Make it happy my lab monkey, and let me test it out, or just put it up to buy! Now get on it, don't make me pull out the whip! O I have an ausome Idea, Bulk Phenogen! And Bulk Leptigen!!!

There was talk of doing bulk Phenogen for one last run (although not through the fine folks at NP- just through us, but I wouldn't be opposed to doing it through NP).

Bulk LeptiGen will probably never happen, but there's talk of coming out with a new version down the road.