Smooth muscle cell genes not known to be regulated by vitamin D analogs are identified

NewsRx.com

06-16-06

A recent study identified genes not previously known to be regulated by VDR, providing insight into understanding the role of VDR on regulating smooth muscle cell growth, thrombogenicity, fibrinolysis and endothelial regeneration.

Researchers in the United States report, "Vitamin D analogs provide survival benefit for chronic kidney disease patients with cardiovascular complications. Activation of smooth muscle cells plays a role in cardiovascular diseases. It is not known how Vitamin D analogs modulate gene expression in smooth muscle cells."

J.R. Wu-Wong and colleagues at Abbott Laboratories explained, "In this study, DNA micro-array technology was used to assess the gene expression profile in human coronary artery smooth muscle cells treated with 0.1 mcM 1alpha,25-dihydroxyvitamin D-3 (calcitriol) or paricalcitol (an analog of calcitriol) for 30 h."

"The effects of calcitriol and paricalcitol were similar," the researchers found. "A total of 176 target genes were identified with 15 up-regulated and 61 down-regulated genes in the paricalcitol group. Target genes fall into various categories including cell differentiation/proliferation. Real-time RT-PCR analysis demonstrated that paricalcitol dose- and time-dependently regulated the expression of IGF1, WT1 and TGF beta3, three genes known to modulate cell proliferation. Paricalcitol also down-regulated the expression of natriuretic peptide precursor B and thrombospondin 1. Both drugs inhibited cell proliferation in a dose-dependent manner."

The researchers concluded, "This study identified genes not previously known to be regulated by VDR, providing insight into understanding the role of VDR on regulating smooth muscle cell growth, thrombogenicity, fibrinolysis and endothelial regeneration."

Wu-Wong and colleagues published their study in the Atherosclerosis (Effects of Vitamin D analogs on gene expression profiling in human coronary artery smooth muscle cells. Atherosclerosis, 2006;186(1):20-28).

For more information, contact J.R. Wuwong, Abbott Laboratories, R4CM, AP52, 200 Abbott Pk Rd., Abbott Pk, IL 60064, USA.

Publisher contact information for the journal Atherosclerosis is: Elsevier Ireland Ltd., Elsevier House, Brookvale Plaza, East Park Shannon, Co. Clare, Ireland.

Keywords: Abbott Park, Illinois, United States, Angiology, Atherosclerosis, Calcitriol, Cardiology, Cardiovascular Disease, DNA Micro-array, DNA Research, Diet and Nutrition, Drugs, Fibrinolysis, Gene Expression, Genetics, Genomics, Human Coronary Artery Smooth Muscle Cells, Paricalcitol, Vascular Disease, Vitamin D Analogs. This article was prepared by Proteomics Weekly editors from staff and other reports.