GDF-8 shRNA

Page 2 of 3 First 123 Last
  1. Board Moderator
    Never enough
    EasyEJL's Avatar
    Stats
    5'10"  205 lbs.
    Join Date
    Jun 2007
    Age
    47
    Posts
    31,868
    Rep Power
    778115
    Level
    95
    Lv. Percent
    27.92%
    Achievements Activity VeteranActivity RoyaltyActivity ProActivity AuthorityPosting Pro

    Quote Originally Posted by Bionic View Post
    What is, Easy?
    just modifying your genes. 30 years from now it won't seem creepy at all. 30 years ago a CAT scan or any of a number of things we routinely do today seemed creepy.

    I really need to look at some studies on myostatins again. this is really intriguing.

  2. Board Moderator
    Never enough
    EasyEJL's Avatar
    Stats
    5'10"  205 lbs.
    Join Date
    Jun 2007
    Age
    47
    Posts
    31,868
    Rep Power
    778115
    Level
    95
    Lv. Percent
    27.92%
    Achievements Activity VeteranActivity RoyaltyActivity ProActivity AuthorityPosting Pro

    what do you think about this? issue with the study of course is these were myostatin deficient from birth, so a bit different

    http://www.pnas.org/cgi/content/abst...urcetype=HWCIT

    and those were also myostatin null mice, no myostatin production at all
  3. New Member
    john777's Avatar
    Join Date
    Mar 2008
    Posts
    2
    Rep Power
    83
    Level
    1
    Lv. Percent
    42%

    What is the price of the:GDF-8 shRNA ?
    •   
       

  4. Senior Member
    Bionic's Avatar
    Stats
    6'5"  227 lbs.
    Join Date
    Jul 2004
    Posts
    1,685
    Rep Power
    986
    Level
    30
    Lv. Percent
    69.88%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by BLACK747 View Post
    that is true but i must say this i when you are actually blocking myostatin its hard to actually eat properly because the hunger effects is out of this world im gonna post some pic next friday back and chest shots tojust let everyone see if there are any progress . the tiredness has been there today so that is one coming effect when you are dealing with this stuff but once i take a nap im back in full effect so to say lol anyway any question from anyone i will try and answer if i can if not i will try and find the answer .
    Fat storage is supposed to be decreased, correct? Sorry to keep referring to the bulls but isn't that why they were so lean? Is this sleepiness something that you'll have to deal with for the log-haul? Maybe that's a question only time will tell. At any rate, that would be a good thing to add to your log (ie: how the sleepiness rates from day to day.) Also, get some quad pics up there, too.
  5. New Member
    mexmuscle's Avatar
    Stats
    5'8"  0 lbs.
    Join Date
    Apr 2008
    Posts
    34
    Rep Power
    98
    Level
    5
    Lv. Percent
    50.24%

    Lets not bother him with questions about sources and prices..... In any case, you can Google whatever information you are looking for.


    Quote Originally Posted by john777 View Post
    What is the price of the:GDF-8 shRNA ?
  6. New Member
    BLACK747's Avatar
    Stats
    5'7"  231 lbs.
    Join Date
    Feb 2006
    Posts
    470
    Rep Power
    342
    Level
    17
    Lv. Percent
    10.32%

    myostatin inhibition do result in astounding fat loss and that is why the bulls are so ripped and i will state how the sleepiness rates from day to day that is something i will keep everyone imformed about
  7. Professional Member
    MentalTwitch's Avatar
    Join Date
    May 2007
    Posts
    4,516
    Rep Power
    2356
    Level
    45
    Lv. Percent
    80.39%
    Achievements Activity ProPosting Pro

    Yea. Theres no source posting/asking, no pricing needs to be talked about.
    Quick over-view...

    1 Dose
    Good pump/full feel
    Extreme Hunger
    A little tired/sleepiness....

    so far i say this is awesome.

    Also, Easy, this isnt creepy, its Fukcin science/bio/chem and it rocks!
    Stem cell research is way more "creepy" if you ask me...
    This is, innovative.

    BLACK, Respect.
    Good work.
    Peace.
  8. Board Moderator
    Never enough
    EasyEJL's Avatar
    Stats
    5'10"  205 lbs.
    Join Date
    Jun 2007
    Age
    47
    Posts
    31,868
    Rep Power
    778115
    Level
    95
    Lv. Percent
    27.92%
    Achievements Activity VeteranActivity RoyaltyActivity ProActivity AuthorityPosting Pro

    How much of the shRNA gets used for that one dose? Now i'm reading about plasmids in general. Funny, that was what they used in the game Bioshock (plasmids)
  9. Professional Member
    MentalTwitch's Avatar
    Join Date
    May 2007
    Posts
    4,516
    Rep Power
    2356
    Level
    45
    Lv. Percent
    80.39%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by EasyEJL View Post
    How much of the shRNA gets used for that one dose? Now i'm reading about plasmids in general. Funny, that was what they used in the game Bioshock (plasmids)
    They come as a set of 4, with 1 negative as a control, if thats what you mean, the unit of measure is a ug. Not sure if it 1ug per tube or what though.
  10. Board Moderator
    Never enough
    EasyEJL's Avatar
    Stats
    5'10"  205 lbs.
    Join Date
    Jun 2007
    Age
    47
    Posts
    31,868
    Rep Power
    778115
    Level
    95
    Lv. Percent
    27.92%
    Achievements Activity VeteranActivity RoyaltyActivity ProActivity AuthorityPosting Pro

    yeah, thats what I thought I saw. i was just wondering what amount of mass that dosage was meant for.
  11. New Member
    mexmuscle's Avatar
    Stats
    5'8"  0 lbs.
    Join Date
    Apr 2008
    Posts
    34
    Rep Power
    98
    Level
    5
    Lv. Percent
    50.24%

    Maybe Mr. Black can tell us how much his research subject used so we can have an idea.:



    Quote Originally Posted by EasyEJL View Post
    yeah, thats what I thought I saw. i was just wondering what amount of mass that dosage was meant for.
  12. Professional Member
    MentalTwitch's Avatar
    Join Date
    May 2007
    Posts
    4,516
    Rep Power
    2356
    Level
    45
    Lv. Percent
    80.39%
    Achievements Activity ProPosting Pro

    Well, i posted something about this on another site i visit alot and has many reputable people, especially in the AAS/Peptide catergory and got this feedabck...
    If he injected himself with a viral vector to silence myostatin, he is a complete idiot. There is so many problems with this, i don't even want to get into it.

    Gene therapy has been almost universally unsuccessful, requires repeated dosing and continued modification of one's genomic DNA, and has resulted in many deaths. For instance, from the induction of cancer.

    I am amazed he got ahold of this. Basically, this is really only available for use in laboratory animals.


    EVERYONE: DO NOT DO THIS. ITS THE MOST STUPID IDEA IMAGINABLE AT THIS POINT.
    If this guy really is doing this, you need to tell hiim that it is very dangerous and it's not permanent, as he believes from the thread. Basically the vector will integrate and express for a few months and then get ****ed up by methylation and DNA excision/repair mechanisms.

    It is very unfortunate if he is actually doing this. Again, this is the kind of thing that's only available for administering to laboratory animals for scientific experiment.
  13. Board Moderator
    Never enough
    EasyEJL's Avatar
    Stats
    5'10"  205 lbs.
    Join Date
    Jun 2007
    Age
    47
    Posts
    31,868
    Rep Power
    778115
    Level
    95
    Lv. Percent
    27.92%
    Achievements Activity VeteranActivity RoyaltyActivity ProActivity AuthorityPosting Pro

    hmm well they are wrong in one aspect, it is available for human use, however its meant for in-vitro use. places I found allowed you to specify rat or human shRNA for a variety of things.
  14. Professional Member
    MentalTwitch's Avatar
    Join Date
    May 2007
    Posts
    4,516
    Rep Power
    2356
    Level
    45
    Lv. Percent
    80.39%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by EasyEJL View Post
    hmm well they are wrong in one aspect, it is available for human use, however its meant for in-vitro use. places I found allowed you to specify rat or human shRNA for a variety of things.
    yes, i did mention that. Im waiting for a response and i did ask him if he'd be willing to sign up here and talk about anything. He is very reputable and experienced and i think he'd be a great addition over here.
  15. Professional Member
    MentalTwitch's Avatar
    Join Date
    May 2007
    Posts
    4,516
    Rep Power
    2356
    Level
    45
    Lv. Percent
    80.39%
    Achievements Activity ProPosting Pro

    You need to find out if he's using a viral vector that integrates into the host genome. It should be something like lentivirus or adenoassociated virus. There aren't a lot of other ways to do what he's purportedly doing, so it likely is a viral vector.

    Next, there has really only been one successful treatment with gene therapy. Something for a liver disease. This means what he's doing hasn't made it through safety and efficacy at the FDA, which means there is no "human grade."
    ...
  16. Board Moderator
    Never enough
    EasyEJL's Avatar
    Stats
    5'10"  205 lbs.
    Join Date
    Jun 2007
    Age
    47
    Posts
    31,868
    Rep Power
    778115
    Level
    95
    Lv. Percent
    27.92%
    Achievements Activity VeteranActivity RoyaltyActivity ProActivity AuthorityPosting Pro

    depends on your definition of "human grade". it is available targeted for human genetic code. part of dna therapy being unsuccessful is that a genetic cause isn't as cut and dried as it is in this particular instance. most genetic anomalies are a case of the gene not functioning properly and the therapy is designed to make the gene work correctly. in this case we are talking about silencing a specific well known gene where we have good documented cases of both the gene working normally, and the gene being silent (the human myostatin deficient cases). So him saying there is no human grade is irrelevant, using that sort of logic there never could be human grade - since if you can't produce material to run FDA testing with, how do you ever get FDA approved?

    granted, this doesn't mean i'd try it
  17. New Member
    BLACK747's Avatar
    Stats
    5'7"  231 lbs.
    Join Date
    Feb 2006
    Posts
    470
    Rep Power
    342
    Level
    17
    Lv. Percent
    10.32%

    listen i have never said anything about this being approved for human use or anything of the sort if you are interested in the results i will post them im not interested in debating someone calling me and idiot this i already know .
  18. Senior Member
    Bionic's Avatar
    Stats
    6'5"  227 lbs.
    Join Date
    Jul 2004
    Posts
    1,685
    Rep Power
    986
    Level
    30
    Lv. Percent
    69.88%
    Achievements Activity ProPosting Pro

    Haha. That last part of the sentence threw me for a loop, Black! Dude, you're doing something that very few have done before, so you know all the "gurus" are going to have something to say. It's already been done and I for one and quite interested in hearing/seeing your results.
  19. Professional Member
    MentalTwitch's Avatar
    Join Date
    May 2007
    Posts
    4,516
    Rep Power
    2356
    Level
    45
    Lv. Percent
    80.39%
    Achievements Activity ProPosting Pro

    I just wanna be clear. In no way was i trying to cut BLACK. He is pioneering and puttin his body on the line for a small community of us, not sure if he is loggin this any where else or will be a future study.

    I simply mentioned it on another board and a member had is opinion over there. I posted it in a neutral position on the topic.

    Sorry for causing any stress on you BLACK or anyone else.
    Let this just carry on.

    peace.
  20. New Member
    BLACK747's Avatar
    Stats
    5'7"  231 lbs.
    Join Date
    Feb 2006
    Posts
    470
    Rep Power
    342
    Level
    17
    Lv. Percent
    10.32%

    no stress homey i just dont want this to turn into a bashing thread thats all
  21. New Member
    BLACK747's Avatar
    Stats
    5'7"  231 lbs.
    Join Date
    Feb 2006
    Posts
    470
    Rep Power
    342
    Level
    17
    Lv. Percent
    10.32%

    and no i only log on anabolicminds baby
  22. Senior Member
    grila jujitsu's Avatar
    Stats
    5'10"  180 lbs.
    Join Date
    Apr 2007
    Age
    29
    Posts
    2,564
    Rep Power
    1374
    Level
    38
    Lv. Percent
    45.21%
    Achievements Activity ProPosting Pro

    so what exactly does this stuff do?
  23. Professional Member
    MentalTwitch's Avatar
    Join Date
    May 2007
    Posts
    4,516
    Rep Power
    2356
    Level
    45
    Lv. Percent
    80.39%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by grila jujitsu View Post
    so what exactly does this stuff do?
    gets you hyyyooooge. hahaha, ill let one of the more well knowledged guys explain.

    PS-I noticed since i started to come here alot more start to wonder here now too....Easy,Bionic, grila...this is an awesome on topic section of the forum guys. enjoy.
  24. Elite Member
    djbombsquad's Avatar
    Stats
    5'5"  150 lbs.
    Join Date
    Dec 2006
    Posts
    6,912
    Rep Power
    365471
    Level
    58
    Lv. Percent
    3.57%
    Achievements Activity ProActivity AuthorityPosting ProPosting Authority

    So you do it once every few weeks? How long does it last? Could one do it Once a month or twice a month?
  25. New Member
    mexmuscle's Avatar
    Stats
    5'8"  0 lbs.
    Join Date
    Apr 2008
    Posts
    34
    Rep Power
    98
    Level
    5
    Lv. Percent
    50.24%

    Quote Originally Posted by djbombsquad View Post
    So you do it once every few weeks? How long does it last? Could one do it Once a month or twice a month?

    Read the thread from the start and you will find the answers.
  26. Elite Member
    djbombsquad's Avatar
    Stats
    5'5"  150 lbs.
    Join Date
    Dec 2006
    Posts
    6,912
    Rep Power
    365471
    Level
    58
    Lv. Percent
    3.57%
    Achievements Activity ProActivity AuthorityPosting ProPosting Authority

    Well so far he only did one but what is optimal?
  27. New Member
    mexmuscle's Avatar
    Stats
    5'8"  0 lbs.
    Join Date
    Apr 2008
    Posts
    34
    Rep Power
    98
    Level
    5
    Lv. Percent
    50.24%

    Quote Originally Posted by djbombsquad View Post
    Well so far he only did one but what is optimal?
    To the best of my knowledge, not enough research has been done for anyone to accurately answer your question.
    Last edited by mexmuscle; 06-23-2008 at 02:48 PM. Reason: Typo
  28. Elite Member
    djbombsquad's Avatar
    Stats
    5'5"  150 lbs.
    Join Date
    Dec 2006
    Posts
    6,912
    Rep Power
    365471
    Level
    58
    Lv. Percent
    3.57%
    Achievements Activity ProActivity AuthorityPosting ProPosting Authority

    I mean I am looking to try it as well with a stack of pegmgf and ghrp6 even if it was just once a month and that gave some results I am game.
  29. Senior Member
    Bionic's Avatar
    Stats
    6'5"  227 lbs.
    Join Date
    Jul 2004
    Posts
    1,685
    Rep Power
    986
    Level
    30
    Lv. Percent
    69.88%
    Achievements Activity ProPosting Pro

    DJ, just chill out and learn something. Why would you be so wiling to do something like this without having anything/one to compare it to/ learn from. Just eat, workout, rest, repeat. Let Black share his experience in peace.
  30. New Member
    babyblu's Avatar
    Join Date
    Oct 2006
    Posts
    474
    Rep Power
    335
    Level
    17
    Lv. Percent
    45.05%

    Quote Originally Posted by djbombsquad View Post
    I mean I am looking to try it as well with a stack of pegmgf and ghrp6 even if it was just once a month and that gave some results I am game.

    Do you have any idea what this stuff is? You are scary as **** to me.

    I have something that will get you swole as ****: eating.

    bb
  31. Professional Member
    MentalTwitch's Avatar
    Join Date
    May 2007
    Posts
    4,516
    Rep Power
    2356
    Level
    45
    Lv. Percent
    80.39%
    Achievements Activity ProPosting Pro

    DJ, thats exactly why i dint answer you. It doesnt seem you research anything.
    Also, BLACK seems to know his shyt pretty well. He is doing this as prolly one of the first and currently only...why would you just go do it? Cause its easy? Quick? i want to too but i wont until there are more guys like BLACK and research is out.

    I meant shyt, we are still finding things out about GHRP, Peg, IGF, etc etc...this log hasent and prolly will not even touch the 1st floor of a 1,000 story building.

    PS-I also dint answer casue a simple google search brings up a major amount of info and labs.

    BLACK: Got the PM, i understand man, no drama is better living.

    be easy.
  32. Senior Member
    Bionic's Avatar
    Stats
    6'5"  227 lbs.
    Join Date
    Jul 2004
    Posts
    1,685
    Rep Power
    986
    Level
    30
    Lv. Percent
    69.88%
    Achievements Activity ProPosting Pro

    Enough with the jibba-jabba. What's cooking, Black?
  33. Senior Member
    grila jujitsu's Avatar
    Stats
    5'10"  180 lbs.
    Join Date
    Apr 2007
    Age
    29
    Posts
    2,564
    Rep Power
    1374
    Level
    38
    Lv. Percent
    45.21%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by MentalTwitch View Post
    gets you hyyyooooge. hahaha, ill let one of the more well knowledged guys explain.

    PS-I noticed since i started to come here alot more start to wonder here now too....Easy,Bionic, grila...this is an awesome on topic section of the forum guys. enjoy.
    Fo sho bra!
  34. Professional Member
    MentalTwitch's Avatar
    Join Date
    May 2007
    Posts
    4,516
    Rep Power
    2356
    Level
    45
    Lv. Percent
    80.39%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by grila jujitsu View Post
    Fo sho bra!
    yea sorry man, dint meanto sound like a dikc, just i dont wanna miss inform. Its complicated stuff...
  35. New Member
    BLACK747's Avatar
    Stats
    5'7"  231 lbs.
    Join Date
    Feb 2006
    Posts
    470
    Rep Power
    342
    Level
    17
    Lv. Percent
    10.32%

    alright sorry about not posting yesterday ate a sandwich from a restaraunt and it got me sick feel better today will get back in the gym tommorrow but all is good know just to make this clear i have never said or will i say that this is approved for human use i just have my personal conviction when it comes to myostatin and if this stuff dont work i will tell everyone it dont work but if it do i will say it does peace!
  36. Professional Member
    CryingEmo's Avatar
    Join Date
    Dec 2006
    Posts
    3,080
    Rep Power
    1636
    Level
    39
    Lv. Percent
    38.95%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by BLACK747 View Post
    alright sorry about not posting yesterday ate a sandwich from a restaraunt and it got me sick feel better today will get back in the gym tommorrow but all is good know just to make this clear i have never said or will i say that this is approved for human use i just have my personal conviction when it comes to myostatin and if this stuff dont work i will tell everyone it dont work but if it do i will say it does peace!

    I'm expecting to see you go from 231 to 300ish... lol
  37. New Member
    BLACK747's Avatar
    Stats
    5'7"  231 lbs.
    Join Date
    Feb 2006
    Posts
    470
    Rep Power
    342
    Level
    17
    Lv. Percent
    10.32%

    we will see
  38. New Member
    BLACK747's Avatar
    Stats
    5'7"  231 lbs.
    Join Date
    Feb 2006
    Posts
    470
    Rep Power
    342
    Level
    17
    Lv. Percent
    10.32%

    well wassup family its your man black another day in the everyday struggle against myostatin so far its been a week since i took the silencer and right know i have to say besides the absolute robust hunger all the time that i am noticing a little belly fat starting to melt away know to put this in perspective i was sick yesterday and the day before and my appetite was all screwed up so that could have something to do with the fat shedding but like my appetite is crazy right know family havent noticed any direct strength gains but we will see in the near future if anything happens
  39. New Member
    BLACK747's Avatar
    Stats
    5'7"  231 lbs.
    Join Date
    Feb 2006
    Posts
    470
    Rep Power
    342
    Level
    17
    Lv. Percent
    10.32%

    alright peeps wassup its your man black back again with another log well its been ten days since my log has started and so far the biggest thing im noticing is belly fat lost and it has been nice losing fat while having and appetite from h###!
    my weight right know is at 230 lbs but i managed to get my waist down from a 36 to a 34 and part of the reason my belly fat was at a 36 anyway is because i ate any and everything and i do mean everything even when i was doing the anti-bodies which i did not do it any justice my main goal on that was strength and for that reason i did not concentrate on cardio at all but i must say it was because of the myostatin anti-bodies that i was not and obese man anyway enough of my mouth oh i know i was supposed to post pic but rest assure they are coming peace!
  40. Professional Member
    CryingEmo's Avatar
    Join Date
    Dec 2006
    Posts
    3,080
    Rep Power
    1636
    Level
    39
    Lv. Percent
    38.95%
    Achievements Activity ProPosting Pro

    Quote Originally Posted by BLACK747 View Post
    well wassup family its your man black another day in the everyday struggle against myostatin

    This is gold. I'm quoting it in my sig.
  

  
 

Similar Forum Threads

  1. Replies: 44
    Last Post: 08-18-2011, 05:17 PM

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
Log in
Log in