GDF-8 shRNA

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  1. Quote Originally Posted by mexmuscle View Post
    Two questions, doubts:

    1. Wasnt this supossed to be shot once only? Wasnt this a single-dose gene changing event?
    He stated that he only took one dose.

  2. Never enough
    EasyEJL's Avatar

    Quote Originally Posted by Bionic View Post
    He stated that he only took one dose.
    it is a little creepy in its own way
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  3. What is, Easy?

  4. Thanks. Dioes anybody know what was the dose?


    Quote Originally Posted by Bionic View Post
    He stated that he only took one dose.

  5. listen im not advising anyone to take this im just sharing my results from day to day since i can tell you this with 100 percent certainty im not gonna have anymore kids its not a worry for me but its possible.know yes this is a one time event and no im not taking anything else

  6. And we thank you for sharing these results BIG BLACK! I hope all is going well so far. Lookin' forward to your updates as always!

    -Papa!-

  7. Dont worry, no one is thinking that you are advising anyone to do that. We are just learning about your experience. I thank you for sharing it and encourage you to continue doing so.

  8. Wow, this is starting to take things to a new level. And I thought using HGH + IGF-1 + PEG-MGF, etc was innovative.


    Black is on the cutting edge.

  9. Yeah Black. You're boldly going where very few have gone before and I applaud your courage and your willingness to share your experience. Cheers! Now get to work and start GROWING!!! LOL
    Now, if I remember correctly, those muscular bulls didn't have to eat more than the regular bulls or workout (obviously) for their muscles to grow huge. Is that what we should expect here? BTW, I know you're going to workout and eat properly. I guess this is hypothetically speaking.

  10. that is true but i must say this i when you are actually blocking myostatin its hard to actually eat properly because the hunger effects is out of this world im gonna post some pic next friday back and chest shots tojust let everyone see if there are any progress . the tiredness has been there today so that is one coming effect when you are dealing with this stuff but once i take a nap im back in full effect so to say lol anyway any question from anyone i will try and answer if i can if not i will try and find the answer .
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  11. Never enough
    EasyEJL's Avatar

    Quote Originally Posted by Bionic View Post
    What is, Easy?
    just modifying your genes. 30 years from now it won't seem creepy at all. 30 years ago a CAT scan or any of a number of things we routinely do today seemed creepy.

    I really need to look at some studies on myostatins again. this is really intriguing.
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  12. Never enough
    EasyEJL's Avatar

    what do you think about this? issue with the study of course is these were myostatin deficient from birth, so a bit different

    http://www.pnas.org/cgi/content/abst...urcetype=HWCIT

    and those were also myostatin null mice, no myostatin production at all
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  13. What is the price of the:GDF-8 shRNA ?

  14. Quote Originally Posted by BLACK747 View Post
    that is true but i must say this i when you are actually blocking myostatin its hard to actually eat properly because the hunger effects is out of this world im gonna post some pic next friday back and chest shots tojust let everyone see if there are any progress . the tiredness has been there today so that is one coming effect when you are dealing with this stuff but once i take a nap im back in full effect so to say lol anyway any question from anyone i will try and answer if i can if not i will try and find the answer .
    Fat storage is supposed to be decreased, correct? Sorry to keep referring to the bulls but isn't that why they were so lean? Is this sleepiness something that you'll have to deal with for the log-haul? Maybe that's a question only time will tell. At any rate, that would be a good thing to add to your log (ie: how the sleepiness rates from day to day.) Also, get some quad pics up there, too.

  15. Lets not bother him with questions about sources and prices..... In any case, you can Google whatever information you are looking for.


    Quote Originally Posted by john777 View Post
    What is the price of the:GDF-8 shRNA ?

  16. myostatin inhibition do result in astounding fat loss and that is why the bulls are so ripped and i will state how the sleepiness rates from day to day that is something i will keep everyone imformed about

  17. Yea. Theres no source posting/asking, no pricing needs to be talked about.
    Quick over-view...

    1 Dose
    Good pump/full feel
    Extreme Hunger
    A little tired/sleepiness....

    so far i say this is awesome.

    Also, Easy, this isnt creepy, its Fukcin science/bio/chem and it rocks!
    Stem cell research is way more "creepy" if you ask me...
    This is, innovative.

    BLACK, Respect.
    Good work.
    Peace.
  18. Never enough
    EasyEJL's Avatar

    How much of the shRNA gets used for that one dose? Now i'm reading about plasmids in general. Funny, that was what they used in the game Bioshock (plasmids)
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  19. Quote Originally Posted by EasyEJL View Post
    How much of the shRNA gets used for that one dose? Now i'm reading about plasmids in general. Funny, that was what they used in the game Bioshock (plasmids)
    They come as a set of 4, with 1 negative as a control, if thats what you mean, the unit of measure is a ug. Not sure if it 1ug per tube or what though.
  20. Never enough
    EasyEJL's Avatar

    yeah, thats what I thought I saw. i was just wondering what amount of mass that dosage was meant for.
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  21. Maybe Mr. Black can tell us how much his research subject used so we can have an idea.:



    Quote Originally Posted by EasyEJL View Post
    yeah, thats what I thought I saw. i was just wondering what amount of mass that dosage was meant for.

  22. Well, i posted something about this on another site i visit alot and has many reputable people, especially in the AAS/Peptide catergory and got this feedabck...
    If he injected himself with a viral vector to silence myostatin, he is a complete idiot. There is so many problems with this, i don't even want to get into it.

    Gene therapy has been almost universally unsuccessful, requires repeated dosing and continued modification of one's genomic DNA, and has resulted in many deaths. For instance, from the induction of cancer.

    I am amazed he got ahold of this. Basically, this is really only available for use in laboratory animals.


    EVERYONE: DO NOT DO THIS. ITS THE MOST STUPID IDEA IMAGINABLE AT THIS POINT.
    If this guy really is doing this, you need to tell hiim that it is very dangerous and it's not permanent, as he believes from the thread. Basically the vector will integrate and express for a few months and then get ****ed up by methylation and DNA excision/repair mechanisms.

    It is very unfortunate if he is actually doing this. Again, this is the kind of thing that's only available for administering to laboratory animals for scientific experiment.
  23. Never enough
    EasyEJL's Avatar

    hmm well they are wrong in one aspect, it is available for human use, however its meant for in-vitro use. places I found allowed you to specify rat or human shRNA for a variety of things.
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  24. Quote Originally Posted by EasyEJL View Post
    hmm well they are wrong in one aspect, it is available for human use, however its meant for in-vitro use. places I found allowed you to specify rat or human shRNA for a variety of things.
    yes, i did mention that. Im waiting for a response and i did ask him if he'd be willing to sign up here and talk about anything. He is very reputable and experienced and i think he'd be a great addition over here.

  25. You need to find out if he's using a viral vector that integrates into the host genome. It should be something like lentivirus or adenoassociated virus. There aren't a lot of other ways to do what he's purportedly doing, so it likely is a viral vector.

    Next, there has really only been one successful treatment with gene therapy. Something for a liver disease. This means what he's doing hasn't made it through safety and efficacy at the FDA, which means there is no "human grade."
    ...
  26. Never enough
    EasyEJL's Avatar

    depends on your definition of "human grade". it is available targeted for human genetic code. part of dna therapy being unsuccessful is that a genetic cause isn't as cut and dried as it is in this particular instance. most genetic anomalies are a case of the gene not functioning properly and the therapy is designed to make the gene work correctly. in this case we are talking about silencing a specific well known gene where we have good documented cases of both the gene working normally, and the gene being silent (the human myostatin deficient cases). So him saying there is no human grade is irrelevant, using that sort of logic there never could be human grade - since if you can't produce material to run FDA testing with, how do you ever get FDA approved?

    granted, this doesn't mean i'd try it
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  27. listen i have never said anything about this being approved for human use or anything of the sort if you are interested in the results i will post them im not interested in debating someone calling me and idiot this i already know .

  28. Haha. That last part of the sentence threw me for a loop, Black! Dude, you're doing something that very few have done before, so you know all the "gurus" are going to have something to say. It's already been done and I for one and quite interested in hearing/seeing your results.

  29. I just wanna be clear. In no way was i trying to cut BLACK. He is pioneering and puttin his body on the line for a small community of us, not sure if he is loggin this any where else or will be a future study.

    I simply mentioned it on another board and a member had is opinion over there. I posted it in a neutral position on the topic.

    Sorry for causing any stress on you BLACK or anyone else.
    Let this just carry on.

    peace.

  30. no stress homey i just dont want this to turn into a bashing thread thats all
  

  
 

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