Problem with reconstitution...rookie looking for help

[disi]

Hello guys,

I am new here and I need quick help. I am sorry if this problem was already solved here somewhere and I will be grateful for help.(I am rookie and I wish I knew your forum earlier as probably I did a big mistake...)(

I bought IGF-1lr from a guy. He told me it was diluted in ba water...he also told that it was better method than using aa. In one vial I have 1ml. I decided to inject into vial another 1ml of ba water so it would be easier for me to take out precise amount of IGF-1. When I injected that additional 1ml of ba some bubbles occurred in the vial and ba did not mix with what was already in the vial. So now it looks like there are two completely different fluids in this vial..one on top of another...they did not mix together at all.

Is there anything I can do now with this IGF-1 or I can simply throw it out, read more and buy other? :think:

 

[Bobaslaw]

Hello guys,

I am new here and I need quick help. I am sorry if this problem was already solved here somewhere and I will be grateful for help.(I am rookie and I wish I knew your forum earlier as probably I did a big mistake...)(

I bought IGF-1lr from a guy. He told me it was diluted in ba water...he also told that it was better method than using aa. In one vial I have 1ml. I decided to inject into vial another 1ml of ba water so it would be easier for me to take out precise amount of IGF-1. When I injected that additional 1ml of ba some bubbles occurred in the vial and ba did not mix with what was already in the vial. So now it looks like there are two completely different fluids in this vial..one on top of another...they did not mix together at all.

Is there anything I can do now with this IGF-1 or I can simply throw it out, read more and buy other? :think:

The longer term integrity of the Peptide depends on a slightly acidic solution, thus AA is used at a concentration of .6% (100mM).
There are some who use BW and BA to reconstitue smaller amounts of IGF (used to be able to get 100mcg vials). That would allow the peptide to be utilized before the IGF was too degraded. In BW or BA I beleive it will not last more than a few days. Still, I did not like this practice along with some protocols that call for freezing IGF reconned in BA or BW. That too will degrade this fragile peptide to some degree, IMO.
The safest most supported method that has the best scientific rational IMO, is to use only AA at the mentioned concentration to recon IGF.

Now, the predicament you are in with the solution being separated, that is unfortunate as I feel your IGF is unusable in this state in addition to the fact it is losing its integrity in BA under normal circumstances anyhow.

Take Care.

 

[disi]

The longer term integrity of the Peptide depends on a slightly acidic solution, thus AA is used at a concentration of .6% (100mM).
There are some who use BW and BA to reconstitue smaller amounts of IGF (used to be able to get 100mcg vials). That would allow the peptide to be utilized before the IGF was too degraded. In BW or BA I beleive it will not last more than a few days. Still, I did not like this practice along with some protocols that call for freezing IGF reconned in BA or BW. That too will degrade this fragile peptide to some degree, IMO.
The safest most supported method that has the best scientific rational IMO, is to use only AA at the mentioned concentration to recon IGF.

Now, the predicament you are in with the solution being separated, that is unfortunate as I feel your IGF is unusable in this state in addition to the fact it is losing its integrity in BA under normal circumstances anyhow.

Take Care.

Ok-thanks bro for help. In meantime I found a topic of other guy on other forum who did the same mistake. He was told there that if he is able to withdraw what he injected his IGF should work. It is true? I am not sure which one is IGF and which one BA...one is more "watery" and sits on top of the more "oily". Do you think I should throw it away or give it a try?

 

[Bobaslaw]

Ok-thanks bro for help. In meantime I found a topic of other guy on other forum who did the same mistake. He was told there that if he is able to withdraw what he injected his IGF should work. It is true? I am not sure which one is IGF and which one BA...one is more "watery" and sits on top of the more "oily". Do you think I should throw it away or give it a try?

Even if the peptide is not damaged (I beleive the IGF is degraded to some extent) there is no way of knowing what concentration of the peptide you are getting buy guessing if you are pulling it from one of the "layers" of solution in the vial. I would not do this.
Not worth messing with it, IMO.