[dsw222]

I have one cc of AA left but want to reconstitute with 2 cc's. Instead of making my own and filtering it, etc... what if I mix 1cc AA with 1cc of bac water? It should still be stable for at least 6 weeks I think. Any opinions?

And .5cc of my AA came from a former board sponser, and I think I read that their AA used to be a higher concentration than .6% anyways.... in which case, the bac water may dilute my solution to an average value of .6%

What do you guys think?

[babyblu]

Just use 1ml of AA. I would NEVER use 2ml of AA to dilute 1mg of igf; that just makes you have to inject MORE AA per microgram of igf.


USING A 1/2 or 1/3 cc slin pin (which has 100ius for 1ml)

1ml of AA + 1mg of igf = 1000mics of igf/100ius of AA

Therefore 10mics of igf/1iu of AA (10 mics of igf in every 1iu of AA)

SO, if you want to run 80mics of igf you would draw up 4ius in 2 slin pins (each). Nice and easy.

bb

[dsw222]

I was going to run 40 mcg each side though, and that seems pretty hard to measure if you only use 1cc. But I will definitely get some smaller volume syringes though, i hadn't thought of that... i was just going to use my 1cc slin pins. I'm guessing its easier to measure small volumes on a smaller volume pin.

thanks for the help!

EDIT: oops you had my dosing right... thanks again

[sam salters]

Originally Posted by dsw222

I was going to run 40 mcg each side though, and that seems pretty hard to measure if you only use 1cc. But I will definitely get some smaller volume syringes though, i hadn't thought of that... i was just going to use my 1cc slin pins. I'm guessing its easier to measure small volumes on a smaller volume pin.

thanks for the help!

EDIT: oops you had my dosing right... thanks again

definately go with the 1/2 cc slin pins, i used 1cc the first time (what i have on hand for HCG) and using the smaller pins makes it much easier to measure out since your using such a tiny amount with IGF-1

[chainsaw]

I contacted GroPep on what they reconstitute thier IGF with as fars as percentage of AA. No answer yet.

[chainsaw]

Got an answer to day from GroPep.

RE: Reconstitution of IGF in 100mM Acetic Acid.‏
From: PWAL (Patricia Wallace) (PWAL@novozymes.com)
Sent: Fri 2/01/08 2:16 AM
To: megan delgros

"IGFs are very stable in acid solution, so there should be no difference which % is used."

So they are saying it doesn't matter, counter argument?

[Xodus]

Originally Posted by chainsaw

Got an answer to day from GroPep.

RE: Reconstitution of IGF in 100mM Acetic Acid.‏
From: PWAL (Patricia Wallace) (PWAL@novozymes.com)
Sent: Fri 2/01/08 2:16 AM
To: megan delgros

"IGFs are very stable in acid solution, so there should be no difference which % is used."

So they are saying it doesn't matter, counter argument?

In your subject line you ask about 100mM AA.

Here is a snippet from Red Baron regarding 5% AA:

Distilled white vinegar is supposed to be standardized to ~5% acetic acid, which would make it 850mM. To get it to the recommended 100mM, you'd want 11.76% white vinegar (100mM/850mM = 11.76%). Since it would be almost impossible to draw out 11.76IU's, I round this to 12, which is certainly going to be close to our desired 100mM.

When diluted with the 7.5:1 (.6%AA) ratio, you get the 100mM concentration.

Bad things:
Possible cellular necrosis.
Wrong pH for peptide.
Burn like hell.

[chainsaw]

Originally I asked

"What do you recommened is the best percentage of Acetic Acid to reconstitute IGF with? Thank you."

They responded

"I don’t know what is the normal % / concentration of a commercially available acetic acid. If you can tell me this, I may be able to help you."

The part about "reconstitution of IGF in 100Mm Acetic Acid" I never asked I don't know why it came back as that message.

[CryingEmo]

Just think if someone decided to sell sterile acetic acid at the right percentage to reconstitute peptides like IGF-1.

They'd be rich!

[sfearl1]

Originally Posted by CryingEmo

Just think if someone decided to sell sterile acetic acid at the right percentage to reconstitute peptides like IGF-1.

They'd be rich!

well, i'm making a buttload of it for OTHER THINGS that don't include reconstituting IGF...

[chainsaw]

Got this reply back from Muscle Research

-----BEGIN PGP SIGNED MESSAGE-----Hash: SHA1 Much much less than 6%. It is a 100mM solution. Not sure off handwhat percentage it works out to but much less than that. About a1/10th of a percent

[Xodus]

Originally Posted by chainsaw

Got this reply back from Muscle Research

Much much less than 6%. It is a 100mM solution. Not sure off handwhat percentage it works out to but much less than that. About a1/10th of a percent

Bingo. 100mM solution works out to ~0.6%

[sfearl1]

x did you reconstitute using the homemade method yet? i'm sitting here with a bottle of distilled water, distilled white vinegar, and two sterile empty vials ready to go. just wondering what you think my best plan of attack is.

[Xodus]

Originally Posted by sfearl1

x did you reconstitute using the homemade method yet? i'm sitting here with a bottle of distilled water, distilled white vinegar, and two sterile empty vials ready to go. just wondering what you think my best plan of attack is.

Not yet, I can't start IGF until after the beta 'test' of the new supp, so ~mid-April.

You got filters?

[sfearl1]

Originally Posted by Xodus

Not yet, I can't start IGF until after the beta 'test' of the new supp, so ~mid-April.

You got filters?

yeah whatman .2um

[Xodus]

Originally Posted by sfearl1

yeah whatman .2um

Cool. It's simple ratios at this point then.

Make sure you vent the vials.

[waderow]

In my experience, IGF reconstituted in AA is a necessity, with dilution in NaCl prior to pinning.
I recently experimented and reconstituted in NaCl with degradation after 1 week. Not good.

Draw IGF into slin Pin, then NaCl as to not contaminate vial.

Use as much NaCl as required for math purposes, and for sting reduction.