Reconstituting IGF-1 LR3

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    Reconstituting IGF-1 LR3


    Any help will be greatly appreciated. This is the first time that I have reconstituted anything. I have ordered an IGF-1 LR3 1 mg vial,when I add 1ml of AA with the IGF it will be 1mg/ml or 1000mcg/ml, is that correct? I am confused as to how many ticks on the insulin syringe would equal 40mcg? It is a 1 ml syringe.I know this is a no brainer, but like I said this is my first time reconstituting and dont want to make a mistake. Would adding 2ml's instead of 1ml be advised to make it easier to measure?

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    Yes...1000 mcgs in your vial. Each tick on the slin pin is 20 mcgs.
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    hmm crunch.. I thought adding 1cc of aa made it so that each tick on the pin was 10mcg so 4 ticks equals 40mcg. If not then i have been using a lot.. But with good results
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    On my pins there are only 5 lines between each 10 marks (10,20, 30...). So if there's only 5, each would be 20.

    But I was a PE major in college, math's not my thing. Maybe somebody here will tell me if I'm right on that. I'm pretty sure though.
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    do you have 1cc or 1/2cc slin pins?
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    His first post say it's a 1ml insulin syringe.
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    Yep, its a 1ml, I believe 1ml is the same volume as 1cc?
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    Quote Originally Posted by I R BABOON View Post
    Yep, its a 1ml, I believe 1ml is the same volume as 1cc?
    You got it.
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    yeah i'm using a 29g 1/2 cc so every tick is 10mcg..

    sorry for the confusion there..
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    Quote Originally Posted by jonesboy View Post
    yeah i'm using a 29g 1/2 cc so every tick is 10mcg..

    sorry for the confusion there..
    I'm generally in a perpetual state of confusion, so I didn't notice.
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    Thank you guys for your help.
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    another question, where do you guys get your NaCl?
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    mine came in the kit.. look at some board sponsors if you need some..
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    Cool, thank you
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    Smile


    Can you reconstitute IGF-1 LR-3 with white vinegar instead of AA?
    A guy from another board recommends it,not better from the regular AA though.
    Thanks.
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    I don't know about using straight Vinegar, but there is a formula for making AA from vinegar. I don't remember who wrote it or I'd give them credit. I pasted it into my AmPad.

    In a 1cc syringe, draw up 0.12cc of distilled white vinegar and 0.88cc of distilled water.
    Remove the needle, put a 0.22ÁM sterile filter, add a sterile needle, and filter that into a sterile vial.

    Well you could also use a 3cc syringe with 0.36cc of AA and 2.64cc of water.
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    Quote Originally Posted by I R BABOON View Post
    another question, where do you guys get your NaCl?
    Is it even necessary? Once re-con'd in AA, why would one even need it?

    Thanks
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    You might want to add NaCl into the pin after you pull your IGF to lessen the sting of the AA. Also, some have reported that AA can actually kill the muscle cells unless diluted, but I don't know how true this is.
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    Thanks. I wonder if it's true?
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    Quote Originally Posted by jonesboy View Post
    yeah i'm using a 29g 1/2 cc so every tick is 10mcg..

    sorry for the confusion there..
    my pins are the exact same (29g 1/2 cc) 50 units....i was assuming 1 unit=1mcg so a full syringe would be 50mcg...are you saying that would be 500mcg?
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    Quote Originally Posted by Tom 185 View Post
    my pins are the exact same (29g 1/2 cc) 50 units....i was assuming 1 unit=1mcg so a full syringe would be 50mcg...are you saying that would be 500mcg?
    thats right every mark on the pin is now 10mcg so a full syringe would be 500mcg.
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    Quote Originally Posted by jonesboy View Post
    thats right every mark on the pin is now 10mcg so a full syringe would be 500mcg.
    hah yea i figured it out once i reconstituted and started yesterday...didnt use any BW...it stung but still very tolerable...my problem is stopping halfway through the injection to pull out and put the other half in the other bi-lateral side of the muscle...its hard to stop accurately
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    try it in the calves. it hurts every time
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    How bad is it if you spray the IGF powder directly a little bit with the AA when you are reconstituting? Like .3 mL worth of AA sprayed directly on the lyophillized powder. It got kind of stuck so i pushed too hard and it squirted in.
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    Does it matter if you use 10% AA or 25% AA solution to reconstitute? I assume if you use bacteriostatic water (.9% BA) in the pin after you draw neither one would sting...
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    AA should be 6% for LR3 IGF-1 reconstitution.
    i was able to find a research co that sells it prediluted to 100 mM (0.6% v/v).
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    Quote Originally Posted by Hank Vangut View Post
    AA should be 6% for LR3 IGF-1 reconstitution.
    i was able to find a research co that sells it prediluted to 100 mM (0.6% v/v).
    So if you get the aa at 6% is that all you need to mix the igf with or do you still need the bw when you draw it for injection?
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    Quote Originally Posted by ocho86 View Post
    So if you get the aa at 6% is that all you need to mix the igf with or do you still need the bw when you draw it for injection?
    yes you do. helps with the sting of the AA
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    Quote Originally Posted by CHA0S View Post
    yes you do. helps with the sting of the AA
    so once i have the reconstituted igf, and i load my syringe, i then draw from the bw?? how much bw? btw, i have 29g 1/2 cc pins, and am doing 30mcg eod (15mcg bilaterally with 2 separate pins post workout). soooo after researching a little further, i am a bit more confused than i thought i was a few weeks back. i had the understanding that 15 ticks on the slin pin = 15 mcg. but a few posts up you guys stated 50 ticks would equal 500 mcg. why would 15 ticks be 150 mcg and not just 15 mcg??? am i totally missing something here?
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    Quote Originally Posted by sfearl1 View Post
    so once i have the reconstituted igf, and i load my syringe, i then draw from the bw?? how much bw? btw, i have 29g 1/2 cc pins, and am doing 30mcg eod (15mcg bilaterally with 2 separate pins post workout). soooo after researching a little further, i am a bit more confused than i thought i was a few weeks back. i had the understanding that 15 ticks on the slin pin = 15 mcg. but a few posts up you guys stated 50 ticks would equal 500 mcg. why would 15 ticks be 150 mcg and not just 15 mcg??? am i totally missing something here?
    i don't think it matters how much bw you draw up. AA destroys muscle tissue so the bw or NaCl is used to dilute it and insure no igf is left in syringe. and i believe the amount of mcg/tick of a slin pin you draw up is directly proportional to the amount of solvent you reconstruct the igf with.
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    I just reconstituted my lr3 and there is a bunch of white powder at the bottom that does not seem to be mixing. Is this a sign my igf is bunk or does this usually happen? I had to keep it lypholised in the fridge for awhile and am wondering if maybe that disrupted it as well? Has this ever happenend to anyone else or does anyone know why this might happen?
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    Quote Originally Posted by ocho86 View Post
    I just reconstituted my lr3 and there is a bunch of white powder at the bottom that does not seem to be mixing. Is this a sign my igf is bunk or does this usually happen? I had to keep it lypholised in the fridge for awhile and am wondering if maybe that disrupted it as well? Has this ever happenend to anyone else or does anyone know why this might happen?
    My powder instantly disolved the second AA touched it. Then I slowly swirled it and let it sit overnight.
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    When I bought from IR they sent me AA that was .10% did I ruin my IGF when I reconstituted. Can you even buy .6% AA anymore?
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    Quote Originally Posted by b_delgros View Post
    When I bought from IR they sent me AA that was .10% did I ruin my IGF when I reconstituted. Can you even buy .6% AA anymore?
    i havent been able to find it. you can make your own but i don't trust myself with filtering something to make it sterile to inject into my body. i'll keep looking
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    Quote Originally Posted by sfearl1 View Post
    i havent been able to find it. you can make your own but i don't trust myself with filtering something to make it sterile to inject into my body. i'll keep looking
    a .45 whatman filter will sterilize the vinegar/distilled water solution.

    If you are that paranoid, you could bake the vial after filtering too.
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    A .22 would be better. Some viruses can pass through a .45 Baking afterwards (boiling is what I do, and it does not blow the tops off the vial) will kill off anything that is left though.
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    Quote Originally Posted by RedwolfWV View Post
    A .22 would be better. Some viruses can pass through a .45 Baking afterwards (boiling is what I do, and it does not blow the tops off the vial) will kill off anything that is left though.
    I thought a .22 was only required for oils and a .45 was for water.
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    Bacteria can live in both. To the best of my knowledge the only way to filter them out for sure is to use a .22
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    Quote Originally Posted by RedwolfWV View Post
    Bacteria can live in both. To the best of my knowledge the only way to filter them out for sure is to use a .22
    Even in AA?
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    I think so, but don't know for sure. Some "critters" are pretty hardy. Maybe someone with more knowledge can chime in.
  

  
 

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