Reconstituting IGF-1 LR3
- 03-02-2007, 04:32 PM
Reconstituting IGF-1 LR3
Any help will be greatly appreciated. This is the first time that I have reconstituted anything. I have ordered an IGF-1 LR3 1 mg vial,when I add 1ml of AA with the IGF it will be 1mg/ml or 1000mcg/ml, is that correct? I am confused as to how many ticks on the insulin syringe would equal 40mcg? It is a 1 ml syringe.I know this is a no brainer, but like I said this is my first time reconstituting and dont want to make a mistake. Would adding 2ml's instead of 1ml be advised to make it easier to measure?
- 03-05-2007, 10:08 AM
Yes...1000 mcgs in your vial. Each tick on the slin pin is 20 mcgs.
- 03-05-2007, 03:20 PM
hmm crunch.. I thought adding 1cc of aa made it so that each tick on the pin was 10mcg so 4 ticks equals 40mcg. If not then i have been using a lot.. But with good results
03-05-2007, 04:01 PM
On my pins there are only 5 lines between each 10 marks (10,20, 30...). So if there's only 5, each would be 20.
But I was a PE major in college, math's not my thing. Maybe somebody here will tell me if I'm right on that. I'm pretty sure though.
03-06-2007, 08:36 AM
03-06-2007, 09:37 AM
His first post say it's a 1ml insulin syringe.
03-06-2007, 12:12 PM
Yep, its a 1ml, I believe 1ml is the same volume as 1cc?
03-06-2007, 01:12 PM
03-06-2007, 02:46 PM
yeah i'm using a 29g 1/2 cc so every tick is 10mcg..
sorry for the confusion there..
03-06-2007, 03:05 PM
03-06-2007, 05:03 PM
03-06-2007, 05:05 PM
another question, where do you guys get your NaCl?
03-06-2007, 05:08 PM
mine came in the kit.. look at some board sponsors if you need some..
03-06-2007, 05:20 PM
04-22-2007, 08:15 AM
Can you reconstitute IGF-1 LR-3 with white vinegar instead of AA?
A guy from another board recommends it,not better from the regular AA though.
04-22-2007, 10:13 AM
I don't know about using straight Vinegar, but there is a formula for making AA from vinegar. I don't remember who wrote it or I'd give them credit. I pasted it into my AmPad.
In a 1cc syringe, draw up 0.12cc of distilled white vinegar and 0.88cc of distilled water.
Remove the needle, put a 0.22ÁM sterile filter, add a sterile needle, and filter that into a sterile vial.
Well you could also use a 3cc syringe with 0.36cc of AA and 2.64cc of water.
04-22-2007, 07:44 PM
04-22-2007, 07:48 PM
You might want to add NaCl into the pin after you pull your IGF to lessen the sting of the AA. Also, some have reported that AA can actually kill the muscle cells unless diluted, but I don't know how true this is.
04-23-2007, 06:14 AM
Thanks. I wonder if it's true?
04-23-2007, 10:58 AM
04-24-2007, 07:49 AM
04-24-2007, 01:54 PM
04-24-2007, 03:14 PM
try it in the calves. it hurts every time
04-26-2007, 03:56 PM
How bad is it if you spray the IGF powder directly a little bit with the AA when you are reconstituting? Like .3 mL worth of AA sprayed directly on the lyophillized powder. It got kind of stuck so i pushed too hard and it squirted in.
07-23-2007, 08:12 AM
Does it matter if you use 10% AA or 25% AA solution to reconstitute? I assume if you use bacteriostatic water (.9% BA) in the pin after you draw neither one would sting...
07-25-2007, 03:28 PM
AA should be 6% for LR3 IGF-1 reconstitution.
i was able to find a research co that sells it prediluted to 100 mM (0.6% v/v).
12-15-2007, 03:43 AM
12-15-2007, 01:17 PM
12-16-2007, 12:54 AM
12-16-2007, 02:42 PM
01-02-2008, 11:27 AM
I just reconstituted my lr3 and there is a bunch of white powder at the bottom that does not seem to be mixing. Is this a sign my igf is bunk or does this usually happen? I had to keep it lypholised in the fridge for awhile and am wondering if maybe that disrupted it as well? Has this ever happenend to anyone else or does anyone know why this might happen?
01-02-2008, 01:42 PM
01-02-2008, 02:54 PM
When I bought from IR they sent me AA that was .10% did I ruin my IGF when I reconstituted. Can you even buy .6% AA anymore?
01-02-2008, 03:46 PM
01-02-2008, 04:38 PM
01-02-2008, 05:39 PM
A .22 would be better. Some viruses can pass through a .45 Baking afterwards (boiling is what I do, and it does not blow the tops off the vial) will kill off anything that is left though.
01-02-2008, 05:52 PM
01-02-2008, 05:58 PM
Bacteria can live in both. To the best of my knowledge the only way to filter them out for sure is to use a .22
01-02-2008, 06:00 PM
01-02-2008, 06:53 PM
I think so, but don't know for sure. Some "critters" are pretty hardy. Maybe someone with more knowledge can chime in.
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