Mechano Growth Factor (MGF) and Insulin-Like Growth Factor-1 (IGF-1) Information Link

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  1. CORRECTION: I read a little yesterday and realized that it was PGE2, not PGE1 (wich is what I was calling it in a previous post) that was the pro inflamatory one that some guys inject in their weiners. It's also one of the ones made by AA conversion via Cox2. Turns out the real PGE1 is actually ANTI-inflamatory and is synthesized from GLA not AA. I think PGF2 is also synthesized from the AA, but the thing I was reading yesterday didnt go into PGF2a synthesis, just PGE1 and PGE2.


  2. Quote Originally Posted by xtraflossy
    Im sure that is would be a GREAT inclusion to a MGF cycle. I was only able to try it some at the end of my IGF cycle, and I didnt overlap that much, so I still had kick-in times of 10+ days berfore the AA should have shown any bennifit.

    Of course, the more the merrier in response to your stacking suggestions, I actually had planned on using MGF with my AA, but the MGF fell through, and I just used the AA.
    Why not IGF-1 with the MGF instead of AA?
    I really would like to make the best profits out of my MGF-cycle, but with the IGF-1 included it would be a pain to my wallet. So if I can get good results with the AA included instead of the IGF-1 this would be great! So I'm interested in your view on this.
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  3. Quote Originally Posted by BassD
    Why not IGF-1 with the MGF instead of AA?
    I really would like to make the best profits out of my MGF-cycle, but with the IGF-1 included it would be a pain to my wallet. So if I can get good results with the AA included instead of the IGF-1 this would be great! So I'm interested in your view on this.
    Lol- I had PLANNED on all 3. I received my LR3, had my AA,.. but the MGF I was expecting never happened.
    Sorry, I wasn't tring to imply that I'd rather use MGF+AA over combining all 3.
    When the new peg. version comes out, I'm going to use that solo though. Turns out, after 2 attempts, I just dont really get anything out of AA

  4. Quote Originally Posted by xtraflossy
    Lol- I had PLANNED on all 3. I received my LR3, had my AA,.. but the MGF I was expecting never happened.
    Sorry, I wasn't tring to imply that I'd rather use MGF+AA over combining all 3.
    When the new peg. version comes out, I'm going to use that solo though. Turns out, after 2 attempts, I just dont really get anything out of AA
    Oh misunderstood you there

    Do you think de PEGylated version will give good results on its own?
  5. Re: Mechano Growth Factor (MGF) and Insulin-Like Growth Factor-1 (IGF-1) Information


    Quote Originally Posted by BassD
    Oh misunderstood you there

    Do you think de PEGylated version will give good results on its own?
    It will definitely be more beneficial in the sense that the half life is going to be extended significantly. Most of the studies conducted by Dr. Goldspink use the PEGylated version due to the fact it has a longer half life, much like LR3 IGF-1 compared to hIGF-1Ea
    PharmD
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  6. Re: Mechano Growth Factor (MGF) and Insulin-Like Growth Factor-1 (IGF-1) Information


    Quote Originally Posted by LakeMountD
    It will definitely be more beneficial in the sense that the half life is going to be extended significantly. Most of the studies conducted by Dr. Goldspink use the PEGylated version due to the fact it has a longer half life, much like LR3 IGF-1 compared to hIGF-1Ea
    Results should be more interesting this time around :bb:

  7. Wow ... great read and awsome work bros. Posted a link on VIP
  8. PEGylated MGF Profile


    PEGylated Mechano Growth Factor (MGF)

    Quick summary: MGF is a splice variant of the IGF produced by a frame shift if the IGF gene. MGF increase the muscle stem cell count, so that more may fuse and become part of adult muscle cells. This is a process required for adult muscle cells to continue growing.

    Why PEGylate MGF?
    MGF exhibits local effects in skeletal muscle and without modification is not systemic (can’t travel through the body). The problem with synthetic MGF is that it is introduced IM and is water based so it goes into the blood stream. MGF is not stable in the blood stream for more than a matter of minutes. Biologically produced MGF is made locally and does not enter the bloodstream and is short acting so stability is not an issue. By PEGylating the MGF we can make synthetic MGF injected IM almost as efficient as local produced MGF. Clinically proven Advanced Pegylation, the technology of polyethylene glycol (PEG) conjugation, holds significant promise in maintaining effective plasma concentrations of systemically administered drugs. It does this by surrounding part of the peptide with a unique structure made of polyethylene glycol, which can be attached to a protein molecule. The result of a correct PEGylation is simlar to the protective mechanism of a turtle shell. The polyethylene glycol groups protect the peptide but don’t surround it completely. The active sites of the peptide are still free to do their biological function. In this case the shell is a negative charged shield against positively charged compounds that would affect the protein. This also provides a nice steric chamber for the peptide to reside in. So it’s a happy turtle

    Neurological research has shown that utilizing PEGylated MGF resulted in a longer more stable acting version of the MGF peptide in serum/blood.

    Bottom line
    PEGylation can improve performance and dosing convenience of peptides, proteins, antibodies, oligonucleotides and many small molecules by optimizing pharmacokinetics, increasing bioavailability, and decreasing immunogenicity and dosing frequency. PEGylation also can increase therapeutic efficacy by enabling increased drug concentration, improved biodistribution, and longer dwell time at the site of action. As a result, therapeutic drug concentrations can be achieved with less frequent dosing—a significant benefit to patients who are taking injected drugs.

    The PEG itself does not react in the body and is very safe. PEG has been approved by the US Food and Drug Administration (FDA) as a base or vehicle for use in foods and cosmetics and in injectable, topical, rectal and nasal pharmaceutical formulations. PEG has demonstrated little toxicity, is eliminated intact by the kidneys or in the feces and lacks immunogenicity. The risk associated with current PEGylated drugs are due to the way the drug itself acts not the PEG. MGF, as it is being currently sold, is getting a bad rep from people due to the fact they feel that they are not seeing gains from it. Many people believe that the use of MGF in their cycles or protocols just flat out won't work, however, this is far from the truth.
    More MGF information
    Complete Overview of MGF or IGF-IEc

    From its sequence, MGF is derived from the IGF-I gene by alternative splicing and has different 3' exons to the liver or systemic type (IGF-IEa). It has a 49 base pair insert in the human, and a 52 base pair insert in rodents, within the E domain of exon 5. This insert results in a reading frame shift, with a different carboxy (C) terminal sequence to that of systemic IGF-IEa. MGF and the other IGF isoforms have the same 5' exons that encode the IGF-I ligand-binding domain. Processing of pro-peptide yields a mature peptide that is involved in upregulating protein synthesis. However, there is evidence that the carboxy-terminal of the MGF peptide also acts as a separate growth factor. This stimulates division of mononucleated myoblasts or satellite (stem) cells, thereby increasing the number available for local repair

    During the early stage of skeletal muscle development, myoblasts (muscle stem cells) fuse to form syncytial myotubes, which become innervated and develop into muscle fibres. Thereafter, mitotic proliferation of nuclei within the muscle fibres ceases. However, during postnatal (after development) growth, additional nuclei are provided by satellite cells (myoblast) fusing with myotubules. Muscle damage-recovery seems to have a similar cellular mechanism, in that satellite cells become activated and fuse with the damaged muscle fibres (reviewed by Goldring et al. 2002). This is also pertinent to certain diseases such as muscular dystrophy in which muscle tissue is not maintained and which have been associated with a deficiency in active satellite (stem) cells (Megeney et al. 1996; Seale & Rudnicki, 2000) and in myogenic factors (Heslop et al. 2000). Skeletal muscle mass and regenerative capacity have also been shown to decline with age (Sadeh, 1988; Carlson et al. 2001). The reduced capacity to regenerate in older muscle seems to be due to the decreased ability to activate satellite cell proliferation (Chakravarthy et al. 2000). The markedly lower expression of MGF in older rat muscles (Owino et al. 2001) and human muscle (Hameed et al. 2003) in response to mechanical overload has been associated with the failure to activate satellite cells, leading to age-related muscle loss (Owino et al. 2001). Your muscle cels can not grow once they have reached a certain size unless they obtain more nuclei from the myoblast. MGF increases the myblast available to donate their nuclei to the adult muscle cell.
    “MGF appears to have a dual action in that, like the other IGF-I isoforms, it upregulates protein synthesis as well as activating satellite cells. However, the latter role of MGF is probably more important as most of the mature IGF-I will be derived from IGF-IEa during the second phase of repair. Nevertheless, it has been shown that MGF is a potent inducer of muscle hypertrophy in experiments in which the cDNA of MGF was inserted into a plasmid vector and introduced by intramuscular injection. This resulted in a 20 % increase in the weight of the injected muscle within 2 weeks, and the analyses showed that this was due to an increase in the size of the muscle fibres (Goldspink, 2001). Similar experiments by other groups have also been carried out using a viral construct containing the liver type of IGF-I, which resulted in a 25 % increase in muscle mass, but this took over 4 months to develop (Musaro et al. 2001). Hence, the dual role MGF plays in inducing satellite cell activation as well as protein synthesis suggests it is much more potent than the liver type or IGF-IEa for inducing rapid hypertrophy.”

    These results are based on actual transplantation of the DNA coding for the peptides. This is a permanent effect and much more potent than IM injections of the peptide itself. You will not see a 20% increase in muscle mass through IM injections as claimed above.
  9. Re: PEGylated MGF Profile


    PEGylated MGF dosing Protocols

    The PEGylated version is going to be much longer lasting making a 1-2 dose per week procedure possible. I still think its best used with IGF or AAS to maximize the benefits so here are some sample protocols

    Once a week PEG MGF/ IGF
    Sunday 100-300 mcg MGF you can choose to site inject if you wish. I think splitting large doses may benefit.
    Monday –Fri IGF 50mcg e/d

    Twice a week PEG MGF / IGF
    Sunday and Wed MGF 50-150 mcg
    MT, ThF IGF 50 mcg

    These protocols are just to start as this is brand new feel free to tweak them if you like. I will update them after we have done some testing.
  10. Re: PEGylated MGF Profile


    Is the Igf-1 totally necessary? Or can we just run MGF on it's on?
  11. Re: PEGylated MGF Profile


    Quote Originally Posted by CHAPS
    Is the Igf-1 totally necessary? Or can we just run MGF on it's on?
    You must remember that MGF only increases the number of staelite cells for use. Naturally you body uses another form of IGF very very similar to the systemic LR3 IGF available to then differentiation these new cells into components of adult muscle cells.

    So is IGF necissary, NO I dont hink you have to have it b/c you have your own natural IGF and cna induce IGF productino through training but I think better results may be acheived using both, or using MGF in conjuction with AAS b/c they also induce differentiation
  12. Re: PEGylated MGF Profile


    Since this continues on the sticky info i'm gonna copy this to the thread up there so there isn't scattered info on this all over the place.
  13. Re: Mechano Growth Factor (MGF) and Insulin-Like Growth Factor-1 (IGF-1) Information


    Ok just keep in mind this is unique to the PEGylated version. Could you edit the title so people know please?

  14. Yeah, no foul meant Game. I've just been working hard on narrowing the info lately to make searching much easier.

  15. Your work is brilliant bro... Thanks for sharing! I have so much to read now... seriously thanks

  16. Quote Originally Posted by LakeMountD
    Still the same info. The problem occurred in Dr. Goldspink's older studies. When he first began research on MGF he hypothesized that it was responsible for not only proliferation (brining in of myoblasts) but also for differentiation (activation of these myoblasts). This, however, proved to not be true in newer studies and actually it was wrong to a very large degree as it was found that not only did MGF not differentiate myoblasts but it actually inhibited myoblasts from differentiating (this is his newest hypothesis). They now say that IGF-1Ea is responsible for the differentiation, which is good for all the LR3 IGF-1 users out there. MGF still has its place as something that has a lot of potential, especially with the longer lasting PEGylated version coming out, but it will take much longer to figure out how to use it synergistically with LR3 IGF-1 since it does inhibit differentiation.

    We basically have to determine how we can dose this stuff to where we get a large influx of myoblasts without inhibiting differentiation. I also still believe LR3 IGF-1 and MGF stacked together are the best way to go. With the large amount of IGF-1 we are getting, we need the extra myoblasts and with all the myoblasts being proliferated by the MGF, you are going to want the added anabolism.
    You seem to be the Resident expert on IGF LR3 and MGF. This will be my first cycle of anything. Ive never been a fan of Steroids, but HGH, IGF, and MGF...interest me do to the fact they are natural functions of the body....just magnified. Any thoughts of a virgin stack of any of these or just alone? I was thinking HGH, but if the size comes for IGF LR3...maybe that is the more appropriate route....with a side of MGF? Thoughts?????

  17. I like LR3 IGF-1 the best personally, but then again have never tried hGH as I could never justify the price and the fact cycles have to be run for very long periods of time. Try getting 1-2mg of LR3 IGF-1 and run it at 20mg EOD or 30mg E3D and let me know how you liked it. Hunger should be way up, increased vascularity (through nitric oxide pathways), decrease in fat, slight increase in muscle mass, but this is the foundation that will be laid for later growth. You can also add in PEG-MGF 2x per week if you'd like. Search around professional muscle . com for some dosage schemes, we had some people trying it out over there who loved it.
    PharmD

  18. Quote Originally Posted by LakeMountD
    I like LR3 IGF-1 the best personally, but then again have never tried hGH as I could never justify the price and the fact cycles have to be run for very long periods of time. Try getting 1-2mg of LR3 IGF-1 and run it at 20mg EOD or 30mg E3D and let me know how you liked it. Hunger should be way up, increased vascularity (through nitric oxide pathways), decrease in fat, slight increase in muscle mass, but this is the foundation that will be laid for later growth. You can also add in PEG-MGF 2x per week if you'd like. Search around professional muscle . com for some dosage schemes, we had some people trying it out over there who loved it.
    Thanks.

    IM...but site specific, bilateral or just quad and glute?

  19. Quote Originally Posted by LakeMountD
    I like LR3 IGF-1 the best personally, but then again have never tried hGH as I could never justify the price and the fact cycles have to be run for very long periods of time. Try getting 1-2mg of LR3 IGF-1 and run it at 20mg EOD or 30mg E3D and let me know how you liked it. Hunger should be way up, increased vascularity (through nitric oxide pathways), decrease in fat, slight increase in muscle mass, but this is the foundation that will be laid for later growth. You can also add in PEG-MGF 2x per week if you'd like. Search around professional muscle . com for some dosage schemes, we had some people trying it out over there who loved it.

    Also, I figure ill reconstitute both with AA. Then what do I backload my slin pins with and how much?

  20. Quote Originally Posted by logan22
    Also, I figure ill reconstitute both with AA. Then what do I backload my slin pins with and how much?
    PEG-MGF is water soluble, no need for AA.
    PharmD

  21. Quote Originally Posted by LakeMountD
    PEG-MGF is water soluble, no need for AA.
    Why EOD or E3D? Why not ED?

  22. Quote Originally Posted by logan22
    Why EOD or E3D? Why not ED?
    Receptor endocytosis, aka receptor down regulation . But search the IGF-1 forum for posts by me and there is a thread about why EOD-E3D injections are best.
    PharmD

  23. Quote Originally Posted by LakeMountD
    Receptor endocytosis, aka receptor down regulation . But search the IGF-1 forum for posts by me and there is a thread about why EOD-E3D injections are best.

    Do you have a link to it? I cant find the posting. So if I pin the MGF of Sunday, (everywhere) Then should I wait one day to pin the LR3, then every other day? Would that work

    Sun MGF 250mcg
    Tues, Thurs, Sat LR3 40mcg?

    How does that sound?

  24. Quote Originally Posted by logan22
    Do you have a link to it? I cant find the posting. So if I pin the MGF of Sunday, (everywhere) Then should I wait one day to pin the LR3, then every other day? Would that work

    Sun MGF 250mcg
    Tues, Thurs, Sat LR3 40mcg?

    How does that sound?
    Day one!

    Started today...20/20mcg bilateral Shoulders. So far so good.

  25. i was thinking of doing a somewhat large shot of mgf (say 200mcg) on sunday, working out mon and tues, wen off and then using igf pwo on thur and fri and maybe sat

    i'd be training each bodypart about twice a week--once on the mgf and once on the igf

    i've run a low dose of igf for 3 on, 4-off before and it worked well

    has anyone tried anything similar yet?
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