Well there are many thought regarding dose and frequency. The standard dose is about 20-50 mcg bi-laterally e.g one injection of 20 mcg in left biceps and 20 mcg in right. Default frequency is three times a week EOD, e.g Mon, Wed, fri. in the muscle(s) you want to increase. This is based on what I have read on different forums and on the net. I also suggest that you use some time to build up knowledge of IGF1, a good start is to take a look at IGF1\GH section here at AM. Where issues,dose, frequency and such is discussed deeply.