Ok guys I have been reading every experts write up in IGF-1 and I still can't get a definitive answer on reconstitution. Everybody seems to say to use BA or acetic acid (1 part 5% vinegar with 7 parts distilled water) and use bac water for cutting it. So is Dave Polumbo an idiot? Why does he tell people that you can use just bact water and the acetic acid is only to get all of the IGF-1 out of the glassware? I want to go with his advice but other experts say the peptide will damage within hours? WTH is the real deal?
Question and Answer with Dave:
4. If i only have bacteriostatic can i just use that to reconstitute? or will it go bad without acetic?
4. It won't go bad sooner, but you will lose some of the IGF-1...the acid helps get it all out of the vial (the lost yield of IGF-1 without using acid water is only a few %)
Dave's IGF-1 Protocol
UNOFFICIAL IGF-1 INSTRUCTIONS
(1) Storage of IGF-1 (prior to mixing)
Lyophilized (dry) IGF-1 is stable at room temperature for 3 weeks; however, it should be stored below -18 degrees Celsius (in the freezer section).
(2) Weight of IGF-1
1 milligram (mg) IGF-1 = 1000 micrograms (mcg) IGF-1
[Dry weight—before mixing]
(3) What to Mix the IGF-1 with
When reconstituting (adding water), It is important to remember that IGF-1 can get “stuck” in the grooves of the glass bottle it comes packaged in. While glass appears smooth to the naked eye, under a microscope, it is a convoluted landscape of grooves and hidden recesses.
By mixing the lyophilized IGF-1 with an “acid water” (e.g. 10mM HCL– very dilute hydrochloric acid), the IGF-1 molecules are efficiently detached from the glass and solubilized in the mixture. Any online “compounding” laboratory could mix up a 10mM HCL solution. Likewise, any intro chemistry student should be able to do the same.
If a reliable source of “acid water” cannot be located, mix your IGF-1 powder with bacteriostatic water—you’ll lose, at worst, 10% of the IGF-1 solution.
(4) Adding the Acid Water
For purposes of mathematical ease, I suggest mixing the dry 1mg (1000mcg) IGF-1 with 3mL (or 3cc) of the “acid water” mixture.
(5) Preservation of the IGF-1
Using a 1cc insulin syringe, draw 1cc out of the bottle that contains 3cc of the acid water/IGF-1 mixture. In a separate 1cc insulin syringe, draw up another 1cc of the solution. Freeze these two loaded insulin syringes. They will be utilized at a later date.
NOTE: Freezing can safely and effectively preserve IGF-1 (even after it’s been mixed).
(6) The Correct Dilution
To the remaining 1cc of IGF-1 that’s left in the glass bottle, add 2cc of bacteriostatic water. This will return the total volume back up to 3cc.
(7) The Mathematics
(a) The original concentration of the IGF-1 solution was 1mg (1000mcg) IGF-1 in 3cc of water.
(b) Each 1cc that we removed, then, contained approximately 333mcg per 1cc.
1000mcg / 3cc = 333mcg per 1cc
(c) The 1cc that was left in the bottle, then, also contains 333mcg of IGF-1.
(d) We added 2cc of bacteriostatic water to the bottle and brought the total volume back up to 3cc. The difference, now, is that we now have 333mcg in 3cc of liquid (instead of in 1cc)
(e) To determine how much IGF-1 is in 1cc, you must divide by 3
333mcg / 3cc = 111mcg per 1cc
(f) To determine how much IGF-1 is in .10cc (or 1/10th cc), we do the following:
111mcg / 10 = 11mcg per .10cc (or 10 unit marking on insulin syringe)
(8) Effective Dosages of IGF-1
Dosages in the range of 10-20mcg per day (taken 15-20 minutes after training) are quite effective for building and repairing muscle tissue. More importantly, these “moderate” dosages (by some people’s estimation) stimulate muscle growth yet escape rapid “downregulation” of the all important IGF-1 receptors. Without receptors to recognize the IGF-1, it doesn’t matter how much you inject.
As dosages climb to over 50mcg per day, receptor downgrade increases exponentially and, from what I’ve observed among bodybuilders, muscle gains come to a screeching halt.
Bodybuilders will have the most success with IGF-1 if they follow the protocol I outlined below. Remember, more isn’t always better!