The effects of Factor A begin to manifest themselves within the first 3-10 days, generally reaching blood level saturation points after 10-15 days of usage. Users of Osteo-Sport will first notice less joint soreness during this time, which can be mostly attribute to Bromelain, Curcumin, and Cissus. Some increase in mobility and joint flexibility is also usually reported during this time period, as there will be some increased lubrication occurring during this period. Factor B begins to become more apparent around Day 15, reaching peak blood levels by Day 20. Users of the compound often report drastic reductions in joint pain by Day 15, and the vast majority of users report markedly increased mobility and joint flexibility during this time, as CMO and Hyaluronic Acid begin to manifest their effects more profoundly. By Day 25, the full scale effects of Factor C kick in, and usually by this time, users of Osteo-Sport report what one customer described as “ a new body.” People who take Osteo-Sport often remark that the reduction in pain and discomfort is so gradual, they usually don’t even notice that it is gone, until they successfully complete some type of activity that was previously restricted by joint pain.
Let’s review the compounds that work most quickly: Cissus, Curcumin, Bromelain, Vitamin C, and Bioperine. Cissus Quadrangularis works rapidly (within 3-5 days), limiting the inflammatory process and by minimizing the activity of harmful cytokines, macro****es, eosinophils, and neutrophils (substances that cause the breakdown of connective tissue) in the joints. This is important, because each of these substances have been implicated as the main factors behind arthritis and joint damage. Numerous published studies on cissus quadrangularis have demonstrated the ability of the herb to effectively suppress the harmful effects of these enzymes.
Curcumin also has the tendency to work very quickly (within 3-8 days), and limit neutrophils and cytokines as well, and also has some unique qualities that could allow the substance to be synergistic with cissus quadrangularis as far as maintaining healthy joints.
Both substances limit cytokines, but cissus seems to do better a slightly better job on limiting neutrophils than curcumin (at least according to some clinical studies) while curcumin has been shown to do a slightly better job in limiting cytokines than cissus. By combining cissus and curcumin, subjects taking both will realize better benefits concerning the alleviation of inflammatory processes than through the use of either product alone. Both entities also enable faster recovery from injury and trauma as cissus and curcumin both mobilize the building blocks of cartilage post-injury, and allow for the re-building of damaged tissue through the same mechanisms of action.
The Effect of Curcumin and Cissus on Cytokines and Neutrophils
Similarly, some recent studies with curcumin have shown that it is very effective for soft tissue repair and wound healing as well. Tissue repair and wound healing are complex processes that involve a series of structural changes in the tissue. These include inflammation, granulation, and remodeling of the tissue. Studies on the effects of curcumin on wound healing showed renewal of the epidermis and increased migration of various cells including myofibroblasts, fibroblasts, and macro****es in the wound bed. Several areas within the dermis showed extensive development of new blood vessels, and greater collagen deposition in curcumin-treated wounds.
Curcumin has also been shown to inhibit the secretion of different enzymes that can slow cartilage regeneration. Curcumin has a marked effect on slowing the expression/secretion of hyaluronidase, the main enzyme that breaks down hyaluronic acid in the skin and joints. This could also represent a potential synergistic effect between curcumin and hyaluronic acid.
Bromelain also has some unique characteristics that allow it to be an effective anti-inflammatory and recovery agent. Bromelain is recognized as an effective treatment for pain and swelling caused by trauma such as sports injuries, surgery, and infections. Studies have demonstrated bromelain’s ability to help the body recover from injury more quickly by preventing the accumulation of fibrin and potent inflammatory substances, such as leukotrienes, cytokines, and neutrophils. The German Expert Panel (Commission E) approved Bromelain for treatment of athletic injuries, recommending a dose of 80 to 120 milligrams two to three times daily.
BROMELAIN AND HEALING
Bromelain also has therapeutic effects in the treatment of inflammation and soft tissue injuries. A clinical trial on bromelain was conducted on boxers with bruises on the face and on the orbits, lips, ears, chest and arms. Bromelain was given to test subjects four times a day for 4 days or until all signs of bruising had disappeared, and a control group of 72 boxers were given a placebo. In 58 of the boxers taking bromelain, all signs of bruising cleared completely in four days, with the remaining 16 requiring 8-10 days for complete clearance. In the control group, only 10 had complete clearance within four days, with the remainder requiring seven to fourteen days for resolution.
Several United States clinical studies also show evidence that taking 1,200 to 1,800 milligrams of bromelain each day can help relive painful inflammation in those that suffer with rheumatoid arthritis. Bromelain, along with cissus and curcumin, has also been shown to work almost right away, and can also increase the effectiveness of other supplements taken along with it.
Bioperine® is also part of the Rapid Release Function of Factor A, mainly because it markedly increases the bioavailability of any substance taken along with it. The more bioavailiable a substance becomes, the faster it can exert its biological effects. A 10-20 mg daily dose of Bioperine® can increase the uptake of Osteo-Sport’s components anywhere from 30-150% (depending on the specific ingredient affected), by allowing the stomach and small intestine to absorb a much greater proportions of the other active ingredients.
Increased Nutrient Uptake of Curcumin with Bioperine®
Vitamin C is important because it converts proline to hydroxyproline, which is the main substance needed for the formation of collagen. Collagen is the most abundant protein on your body, and is a very important structural element in connective tissue. Having the appropriate amount of vitamin C in your system ensures that users of Osteo-Sport™ will have the necessary amount of this important the building block for collagen production, allowing for the product to have maximum effectiveness in a time-efficient manner.
Factor B (Intermediate-Release Factor) contributes to the overall effectiveness of Osteo-Sport in a synergistic, timed manner that improves with continued use. Factor B is comprised of CMO, Hyaluronic Acid, and MSM, which generally take from 10-21 days to begin exerting their complementary effects.
Hyaluronic acid is a unique compound, in that it cushions the joints, and promotes nutrient and waste circulation to and from existing cartilage. Hyaluronic acid is essential for the health of the synovial fluid which supports joints and lubricates tendons. Numerous small studies have shown excellent results from oral administration of hyaluronic acid:
1. In a human study, an oral mixture of sodium hyaluronate (10 mg) and chondroitin sulfate (100 mg) for three months accelerated healing from knee surgery in all 27 test subjects in comparison with 27 control subjects.
2. A daily oral dose of 40 mg hyaluronic acid in capsules was administered to 100 women for 45 days in a study conducted at Ohtsuma University in Tokyo. Skin texture was “remarkably” improved in 75%-81% of patients; eyesight was “remarkably” improved in 25; joint stiffness was "remarkably" improved in 48. The entire remainder of the study group was “better” in all result categories.
3. A similar study conducted on 28 patients taking 30 mg oral hyaluronic acid for 30 days found 93% improved their joint pain symptoms.
Hyaluronic acid also seems to be synergistic with curcumin, as dosing curcumin can actually de-activate the enzyme that breaks down hyaluronic acid, allowing for much greater levels of hyaluronic acid in the blood.
Chondrotin sulfate has also been shown to have some effect in boosting
hyaluronic acid production, therefore promoting even greater joint lubrication.
Another component of Factor B is cetylmyristoleate (CMO), an ester of a fatty acid that increases the lubrication, longevity, and resilience of cartilage and other tissues. Fatty acids are the individual components of oils in the same way that amino acids are the building blocks of proteins. CMO is a combination of cetyl alcohol, a long chain alcohol molecule, and myristoleic acid, a fatty acid. Cetylmyristoleate seems to function in three very different capacities. It lubricates not only the involved joints, but also lubricates the entire body, making muscles glide more smoothly over other muscles, bursas, and bones and at the same time softens these tissues, making them more pliable.