# IGF reconstitution and 3/10 insulin pins?

#### stankydanky

##### New member
Okay so i added 1ml of AA to 1mg of IGF-1. Using 29g 3/10cc slin pins how much is each tick in terms of mcg? 10 right? Thanks

#### papapumpsd

##### Well-known member
Okay so i added 1ml of AA to 1mg of IGF-1. Using 29g 3/10cc slin pins how much is each tick in terms of mcg? 10 right? Thanks
I just discovered you could get the 0.3cc slinpins. Those must be darn small!

Sorry, not sure about the ticks on that. Pumbert or Bobas will probably let you know.

BTW, did you make your own filtered 0.6%AA?

#### Bobaslaw

##### Member
Okay so i added 1ml of AA to 1mg of IGF-1. Using 29g 3/10cc slin pins how much is each tick in terms of mcg? 10 right? Thanks
Here is a pic of the 3/10cc Insulin Syringe scale...

1cc=1ml. There are 10 "tics" per .10 (tenth) cc. 1mg/ml (1000mcg/ml) is equal to 100mcg per .10 cc, which would give you 10mcg per tic.

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#### papapumpsd

##### Well-known member
Here is a pic of the 3/10cc Insulin Syringe scale...

1cc=1ml. There are 10 "tics" per .10 (tenth) cc. 1mg/ml (1000mcg/ml) is equal to 100mcg per .10 cc, which would give you 10mcg per tic.
Ah yes, very good. This makes clear sense. I was under the impression that some goofy IU conversion was needed in figuring this out. But it's apparent now that they're meaningless really. I never used IU's in lab so was unfamiliar with that unit.

This is simple dimensional analysis:
Your goal is to determine mcg/tick:
1. 30 ticks/0.3mL = 100ticks/1mL
2. Thus, 1mL/100 ticks
3. (1mL/100ticks) x (1,000mcg/mL). (remember, 1,000mcg/mg)
4. The mL's cancel out, leaving 1,000mcg/100ticks = 10mcg/tick

I'm simply reiterating what Bobas said.

FYI: If you're using 0.5mL (0.5cc) slinpins (as I am), the calculations result in the same 10mcg/tick as found above! The slinpins will have 50 ticks and will hold 0.5mL, so ticks/mL = 100ticks/1mL ==>(invert) ==> 1mL/100ticks.

#### Bobaslaw

##### Member
Ah yes, very good. This makes clear sense. I was under the impression that some goofy IU conversion was needed in figuring this out. But it's apparent now that they're meaningless really. I never used IU's in lab so was unfamiliar with that unit.

This is simple dimensional analysis:
Your goal is to determine mcg/tick:
1. 30 ticks/0.3mL = 100ticks/1mL
2. Thus, 1mL/100 ticks
3. (1mL/100ticks) x (1,000mcg/mL). (remember, 1,000mcg/mg)
4. The mL's cancel out, leaving 1,000mcg/100ticks = 10mcg/tick

I'm simply reiterating what Bobas said.

FYI: If you're using 0.5mL (0.5cc) slinpins (as I am), the calculations result in the same 10mcg/tick as found above! The slinpins will have 50 ticks and will hold 0.5mL, so ticks/mL = 50ticks/1mL ==>(invert) ==> 1mL/50ticks.
Ya Papa, ppl confuse IUs and throw it out in their conversions and slin pin measurements to describe "volume".
International Units are a "mass" measurement not a volume measurement.

http://en.wikipedia.org/wiki/International_unit

In pharmacology, the International Unit is a unit of measurement for the amount of a substance, based on measured biological activity or effect.

#### stankydanky

##### New member
okay i made my own AA, composed of 1ml of white vinegar (5% acidic) and 7ml of distilled water. After filtering into a sterile vial i drew 1ml out of the vial (Which is AA) and added it to the 1mg of IGF. Im pretty sure i did everything correctly, will it ruin the IGF if a few drops were missed when making the AA?

#### papapumpsd

##### Well-known member
okay i made my own AA, composed of 1ml of white vinegar (5% acidic) and 7ml of distilled water. After filtering into a sterile vial i drew 1ml out of the vial (Which is AA) and added it to the 1mg of IGF. Im pretty sure i did everything correctly, will it ruin the IGF if a few drops were missed when making the AA?
A few drops of AA were missed? Or a few drops of distilled H2O? I think you're already low at 7mLs of H2O (I will use 7.5mL). Anyways, it's all not THAT critical in my opinion. It all boils down to acidity of your IGF-1/AA soln. If you use less than 7.0mL H2O, the IGF-1 soln. will be more acidic and you should back-load with more BW to dilute the acid.

I'm not saying that greater acidity is better. And IMO it won't ruin your IGF if a few "drops" were missed (assuming it was water). The acidity is to ward off bacterial growth.

My \$.02

#### buster12

##### Member
Here is a pic of the 3/10cc Insulin Syringe scale...

1cc=1ml. There are 10 "tics" per .10 (tenth) cc. 1mg/ml (1000mcg/ml) is equal to 100mcg per .10 cc, which would give you 10mcg per tic.
poop

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