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Ok, you wanted to know what's in it. Sometimes simple is awesome and here's what we have for you. Full write up will be a bit later:
Write Up Abstract: D-Aspartic Acid is well known to increase testosterone production. We wanted to see if we could get an improvement on this and boost testosterone levels even higher along with increasing growth hormone as well. Lactate is a little known stimulator of hormones. It is theorized that after strenuous exercise, the body dumps a small amount of lactate into the blood as a signal mechanism to tell it to increase the supply of muscle building hormones. This signaling pathway makes total sense when you think about it! Lactate increased testicular production of testosterone similar to HCG. (1,2) Also may have a profound effect on growth hormone (3).
This is why we say that this product truly is exercise in a bottle from a hormonal perspective. People are coming around to the understanding that blood lactate is indeed a potent stimulator of hormones (will post other articles)
"Lactic acid does not cause that dreaded burning sensation during intense exercise. When lactic acid is made it’s split into lactate ion (lactate) and hydrogen ion. Hydrogen ion is the bad guy, the acid in lactic acid that interferes with electrical signals in nerve and muscle tissue. And lactic acid is not responsible for any muscle soreness felt the next day or two after a hard workout. That is caused by muscle damage and post-exercise tissue inflammation."
So you see, it's not lactate it's the H- that causes the muscle "burn". You get the benefits of the lactate without the negatives of the H atom!
We looked for the best form of Lactate to give with our D-AA. Well, luckily we found a supplier of Calcium Lactate which gives us both lactate and calcium. Calcium is an important cofactor in testosterone production (4). Now, perhaps you need more calcium but then again perhaps not...
DA-HCG - 1 Full Month Supply (4 Weeks)
Servings Per Container: ~56
2 Scoops (Filled to Fill Line) Equals:
3g D-Aspartic Acid
1.5g Calcium Lactate (Supplying 1.3g Lactate)
Directions: Fill Scoop To Serving Line For Each Dose. Take 2 Scoops (Filled Only To Scoop Fill Line) Per Day On An Empty Stomach Between Meals Divided Into Two Doses. Cycle Off The Product For At Least 1 Week After Each Container.
D-Aspartic Acid is well studied in cellular metabolism to boost testosterone production via important testicular enzymes. Lactate is a signaling molecule used by the body after intense exercise to stimulate growth and hormone release. Lactacte has been shown in preliminary studies to work in conjunction with D-Aspartic Acid to increase testosterone production, as well as increasing IGF-1 and Growth Hormone Levels.
Brief Info:
Check out these studies on lactate stimulating testosterone similar to HCG:
1) Lin H, Wang SW, Wang RY, Wang PS. J Cell Biochem. 2001 Jun 26-Jul 25;83(1):147-54. Stimulatory effect of lactate on testosterone production by rat Leydig cells.
Previously we found that the increased plasma testosterone levels in male rats during exercise partially resulted from a direct and luteinizing hormone (LH)-independent stimulatory effect of lactate on the secretion of testosterone. In the present study, the acute and direct effects of lactate on testosterone production by rat Leydig cells were investigated. Leydig cells from rats were purified by Percoll density gradient centrifugation subsequent to enzymatic isolation of testicular interstitial cells. Purified rat Leydig cells (1 x 10(5) cells/ml) were in vitro incubated with human chorionic gonadotropin (hCG, 0.05 IU/ml), forskolin (an adenylyl cyclase activator, 10(-5) M), or 8-bromo-adenosine-3':5'-cyclic monophosphate (8-Br-cAMP, 10(-4) M), SQ22536 (an adenylyl cyclase inhibitor, 10(-6)-10(-5) M), steroidogenic precursors (25-hydroxy-cholesterol, pregnenolone, progesterone, and androstenedione, 10(-5) M each), nifedipine (a L-type Ca(2+) channel blocker, 10(-5)-10(-4) M), or nimodipine (a potent L-type Ca(2+) channel antagonist, 10(-5)-10(-4) M) in the presence or absence of lactate at 34 degrees C for 1 h. The concentration of medium testosterone was measured by radioimmunoassay. Administration of lactate at 5-20 mM dose-dependently increased the basal testosterone production by 63-187% but did not alter forskolin- and 8-Br-cAMP-stimulated testosterone release in rat Leydig cells. Lactate at 10 mM enhanced the stimulation of testosterone production induced by 25-hydroxy-cholesterol in rat Leydig cells but not other steroidogenic precursors. Lactate (10 mM) affected neither 30- nor 60-min expressions of cytochrome P450 side chain cleavage enzyme (P450scc) and steroidogenic acute regulatory (StAR) protein. The lactate-stimulated testosterone production was decreased by administration of nifedipine or nimodipine. These results suggested that the physiological level of lactate stimulated testosterone production in rat Leydig cells through a mechanism involving the increased activities of adenylyl cyclase, cytochrome P450scc, and L-type Ca(2+) channel.
2) Lu SS, Lau CP, Tung YF, Huang SW, Chen YH Med Sci Sports Exerc. 1997 Aug;29(8):1048-54. Lactate and the effects of exercise on testosterone secretion: evidence for the involvement of a cAMP-mediated mechanism.
The effects of swimming and lactate on the release of testosterone were examined in male rats. During in vivo experiments, male rats were catheterized via the right jugular vein and blood was collected at 0, 10, 15, 30, and 60 min following the exercise, or they were catheterized via the right jugular vein and the left femoral vein and blood was collected at 0, 2, 5, 10, 15, 30, 60, and 120 min after a 10-min infusion at lactate (13 mg.kg-1.min-1). Trunk blood and blood from the testicular vein were also collected after 10 min of swimming or water immersion. In an in vitro experiment, testicular fragments were challenged with lactate (0.01-10 mM) and/or human chorionic gonadotropin (hCG; 0.5 IU.mL-1), and the mediobasal hypothalamus (MBH) was challenged with lactate (8 mM). The post-exercise levels of plasma lactate and testosterone at 10, 15, and 30 min were higher than resting levels. Plasma luteinizing hormone (LH) was increased following 30 min of swimming. Administration of lactate or hCG increased in a dose dependent manner testicular cyclic adenosine 3':5' monophosphate (cAMP) and testosterone release. Plasma testosterone increased after swimming and lactate infusion. Incubation of MBH with lactate increased the gonadotropin-releasing hormone (GnRH) level in the medium. These results suggest that the increased plasma testosterone levels in male rats during exercise is at least partially a result of a direct and LH-independent stimulatory effect of lactate on the secretion of testosterone by increasing testicular cAMP production. Swim-elevated plasma LH may be a result of a rise of GnRH caused by lactate.
3) Godfrey RJ, Whyte GP, Buckley J, Quinlivan R. Br J Sports Med. 2009 Jul;43(7):521-5. Epub 2008 Jan 9. The role of lactate in the exercise-induced human growth hormone response: evidence from McArdle disease.
PURPOSE: Increased blood lactate concentration has been suggested as a primary stimulus for the exercise-induced growth hormone response (EIGR). Patients with McArdle disease are unable to produce lactate in response to exercise and thus offer a unique model to assess the role of lactate in the EIGR. Accordingly, McArdle's patients were exercised to test the hypothesis that lactate is a major stimulus of the EIGR.
METHODS: 11 patients with McArdle disease (3 male, 8 female; age: 35.5 (SD 13.9) years, height: 166 (8) cm, body mass: 75.2 (13.1) kg) were recruited for the study. The patients walked initially at 0.42 m/s, increasing by 0.14 m/s per 3 min stage. Exercise was terminated when participants completed 3 minutes at 1.80 m/s or when a Borg CR10 pain scale rating of "4" was reached. Stages were separated by 60 s for capillary blood sampling for analysis of hGH and blood lactate concentration.
RESULTS: McArdle's patients' blood lactate levels remained at resting levels (0.3-1.2 mmol/l) as exercise intensity increased. Nine out of 11 participants failed to demonstrate an EIGR obtaining hGH values below the clinical definition of a response (>3 microg/l).
CONCLUSION: The absence of an EIGR in nine out of 11 participants suggests that lactate could play a major role in the EIGR in humans.
4) Cinar V, Baltaci AK, Mogulkoc R, Kilic M. Biol Trace Elem Res. 2009 Summer;129(1-3):65-9. Epub 2008 Dec 20. Testosterone levels in athletes at rest and exhaustion: effects of calcium supplementation.
Abstract
The effects of 4 weeks of calcium supplementation on free- and total testosterone levels were established in active and sedentary adult males at rest and exhaustion. Thirty healthy male athletes were equally divided into three study groups, as follows: Group 1-non-exercising subjects receiving 35 mg calcium/kg body weight; Group 2-subjects receiving 35 mg calcium/kg body weight undergoing training routines for 90 min/day, 5 days a week and Group 3-subjects undergoing training routines for 90 min/day, 5 days a week. The testosterone levels were determined before and after supplementation, at rest and following a hard training routine. The plasma free- and total testosterone levels increased at exhaustion before and after supplementation relative to resting values (p < 0.05). This was also true when active subjects were compared to inactive subjects (p < 0.05). Our results show that training results in increased testosterone levels in athletes and that the increase is greater if accompanied by calcium supplementation, which may be useful for increasing overall athletic performance.
Write Up Abstract: D-Aspartic Acid is well known to increase testosterone production. We wanted to see if we could get an improvement on this and boost testosterone levels even higher along with increasing growth hormone as well. Lactate is a little known stimulator of hormones. It is theorized that after strenuous exercise, the body dumps a small amount of lactate into the blood as a signal mechanism to tell it to increase the supply of muscle building hormones. This signaling pathway makes total sense when you think about it! Lactate increased testicular production of testosterone similar to HCG. (1,2) Also may have a profound effect on growth hormone (3).
This is why we say that this product truly is exercise in a bottle from a hormonal perspective. People are coming around to the understanding that blood lactate is indeed a potent stimulator of hormones (will post other articles)
"Lactic acid does not cause that dreaded burning sensation during intense exercise. When lactic acid is made it’s split into lactate ion (lactate) and hydrogen ion. Hydrogen ion is the bad guy, the acid in lactic acid that interferes with electrical signals in nerve and muscle tissue. And lactic acid is not responsible for any muscle soreness felt the next day or two after a hard workout. That is caused by muscle damage and post-exercise tissue inflammation."
So you see, it's not lactate it's the H- that causes the muscle "burn". You get the benefits of the lactate without the negatives of the H atom!
We looked for the best form of Lactate to give with our D-AA. Well, luckily we found a supplier of Calcium Lactate which gives us both lactate and calcium. Calcium is an important cofactor in testosterone production (4). Now, perhaps you need more calcium but then again perhaps not...
DA-HCG - 1 Full Month Supply (4 Weeks)
Servings Per Container: ~56
2 Scoops (Filled to Fill Line) Equals:
3g D-Aspartic Acid
1.5g Calcium Lactate (Supplying 1.3g Lactate)
Directions: Fill Scoop To Serving Line For Each Dose. Take 2 Scoops (Filled Only To Scoop Fill Line) Per Day On An Empty Stomach Between Meals Divided Into Two Doses. Cycle Off The Product For At Least 1 Week After Each Container.
D-Aspartic Acid is well studied in cellular metabolism to boost testosterone production via important testicular enzymes. Lactate is a signaling molecule used by the body after intense exercise to stimulate growth and hormone release. Lactacte has been shown in preliminary studies to work in conjunction with D-Aspartic Acid to increase testosterone production, as well as increasing IGF-1 and Growth Hormone Levels.
Brief Info:
Check out these studies on lactate stimulating testosterone similar to HCG:
1) Lin H, Wang SW, Wang RY, Wang PS. J Cell Biochem. 2001 Jun 26-Jul 25;83(1):147-54. Stimulatory effect of lactate on testosterone production by rat Leydig cells.
Previously we found that the increased plasma testosterone levels in male rats during exercise partially resulted from a direct and luteinizing hormone (LH)-independent stimulatory effect of lactate on the secretion of testosterone. In the present study, the acute and direct effects of lactate on testosterone production by rat Leydig cells were investigated. Leydig cells from rats were purified by Percoll density gradient centrifugation subsequent to enzymatic isolation of testicular interstitial cells. Purified rat Leydig cells (1 x 10(5) cells/ml) were in vitro incubated with human chorionic gonadotropin (hCG, 0.05 IU/ml), forskolin (an adenylyl cyclase activator, 10(-5) M), or 8-bromo-adenosine-3':5'-cyclic monophosphate (8-Br-cAMP, 10(-4) M), SQ22536 (an adenylyl cyclase inhibitor, 10(-6)-10(-5) M), steroidogenic precursors (25-hydroxy-cholesterol, pregnenolone, progesterone, and androstenedione, 10(-5) M each), nifedipine (a L-type Ca(2+) channel blocker, 10(-5)-10(-4) M), or nimodipine (a potent L-type Ca(2+) channel antagonist, 10(-5)-10(-4) M) in the presence or absence of lactate at 34 degrees C for 1 h. The concentration of medium testosterone was measured by radioimmunoassay. Administration of lactate at 5-20 mM dose-dependently increased the basal testosterone production by 63-187% but did not alter forskolin- and 8-Br-cAMP-stimulated testosterone release in rat Leydig cells. Lactate at 10 mM enhanced the stimulation of testosterone production induced by 25-hydroxy-cholesterol in rat Leydig cells but not other steroidogenic precursors. Lactate (10 mM) affected neither 30- nor 60-min expressions of cytochrome P450 side chain cleavage enzyme (P450scc) and steroidogenic acute regulatory (StAR) protein. The lactate-stimulated testosterone production was decreased by administration of nifedipine or nimodipine. These results suggested that the physiological level of lactate stimulated testosterone production in rat Leydig cells through a mechanism involving the increased activities of adenylyl cyclase, cytochrome P450scc, and L-type Ca(2+) channel.
2) Lu SS, Lau CP, Tung YF, Huang SW, Chen YH Med Sci Sports Exerc. 1997 Aug;29(8):1048-54. Lactate and the effects of exercise on testosterone secretion: evidence for the involvement of a cAMP-mediated mechanism.
The effects of swimming and lactate on the release of testosterone were examined in male rats. During in vivo experiments, male rats were catheterized via the right jugular vein and blood was collected at 0, 10, 15, 30, and 60 min following the exercise, or they were catheterized via the right jugular vein and the left femoral vein and blood was collected at 0, 2, 5, 10, 15, 30, 60, and 120 min after a 10-min infusion at lactate (13 mg.kg-1.min-1). Trunk blood and blood from the testicular vein were also collected after 10 min of swimming or water immersion. In an in vitro experiment, testicular fragments were challenged with lactate (0.01-10 mM) and/or human chorionic gonadotropin (hCG; 0.5 IU.mL-1), and the mediobasal hypothalamus (MBH) was challenged with lactate (8 mM). The post-exercise levels of plasma lactate and testosterone at 10, 15, and 30 min were higher than resting levels. Plasma luteinizing hormone (LH) was increased following 30 min of swimming. Administration of lactate or hCG increased in a dose dependent manner testicular cyclic adenosine 3':5' monophosphate (cAMP) and testosterone release. Plasma testosterone increased after swimming and lactate infusion. Incubation of MBH with lactate increased the gonadotropin-releasing hormone (GnRH) level in the medium. These results suggest that the increased plasma testosterone levels in male rats during exercise is at least partially a result of a direct and LH-independent stimulatory effect of lactate on the secretion of testosterone by increasing testicular cAMP production. Swim-elevated plasma LH may be a result of a rise of GnRH caused by lactate.
3) Godfrey RJ, Whyte GP, Buckley J, Quinlivan R. Br J Sports Med. 2009 Jul;43(7):521-5. Epub 2008 Jan 9. The role of lactate in the exercise-induced human growth hormone response: evidence from McArdle disease.
PURPOSE: Increased blood lactate concentration has been suggested as a primary stimulus for the exercise-induced growth hormone response (EIGR). Patients with McArdle disease are unable to produce lactate in response to exercise and thus offer a unique model to assess the role of lactate in the EIGR. Accordingly, McArdle's patients were exercised to test the hypothesis that lactate is a major stimulus of the EIGR.
METHODS: 11 patients with McArdle disease (3 male, 8 female; age: 35.5 (SD 13.9) years, height: 166 (8) cm, body mass: 75.2 (13.1) kg) were recruited for the study. The patients walked initially at 0.42 m/s, increasing by 0.14 m/s per 3 min stage. Exercise was terminated when participants completed 3 minutes at 1.80 m/s or when a Borg CR10 pain scale rating of "4" was reached. Stages were separated by 60 s for capillary blood sampling for analysis of hGH and blood lactate concentration.
RESULTS: McArdle's patients' blood lactate levels remained at resting levels (0.3-1.2 mmol/l) as exercise intensity increased. Nine out of 11 participants failed to demonstrate an EIGR obtaining hGH values below the clinical definition of a response (>3 microg/l).
CONCLUSION: The absence of an EIGR in nine out of 11 participants suggests that lactate could play a major role in the EIGR in humans.
4) Cinar V, Baltaci AK, Mogulkoc R, Kilic M. Biol Trace Elem Res. 2009 Summer;129(1-3):65-9. Epub 2008 Dec 20. Testosterone levels in athletes at rest and exhaustion: effects of calcium supplementation.
Abstract
The effects of 4 weeks of calcium supplementation on free- and total testosterone levels were established in active and sedentary adult males at rest and exhaustion. Thirty healthy male athletes were equally divided into three study groups, as follows: Group 1-non-exercising subjects receiving 35 mg calcium/kg body weight; Group 2-subjects receiving 35 mg calcium/kg body weight undergoing training routines for 90 min/day, 5 days a week and Group 3-subjects undergoing training routines for 90 min/day, 5 days a week. The testosterone levels were determined before and after supplementation, at rest and following a hard training routine. The plasma free- and total testosterone levels increased at exhaustion before and after supplementation relative to resting values (p < 0.05). This was also true when active subjects were compared to inactive subjects (p < 0.05). Our results show that training results in increased testosterone levels in athletes and that the increase is greater if accompanied by calcium supplementation, which may be useful for increasing overall athletic performance.
