in the brain*?
May I ask what this accomplishes? Sounds interesting...
Anything that increases Glut-1 activity- glucose is preferentially stored in brain first b/c of this m.o.a.....Glut-1 receptors are thought to form the blood/brain barrier
J. Biol. Chem., Vol. 265, Issue 29, 18035-18040, 10, 1990
Brain-type glucose transporter (GLUT-1) is selectively localized to the blood-brain barrier. Studies with quantitative western blotting and in situ hybridization
WM Pardridge, RJ Boado and CR Farrell
Department of Medicine, UCLA School of Medicine 90024.
The hypothesis that the GLUT-1 glucose transporter isoform is expressed selectively in brain at the capillary endothelium, i.e. the blood-brain barrier (BBB), was tested by using quantitative Western blotting, cytochalasin B binding, and in situ hybridization in bovine brain cortex. Purified human glucose transporter was used as the standard for quantitative Western blots, because the mobility of the human erythrocyte and BBB glucose transporters in electrophoretic gels was identical. The concentration of immunoreactive glucose transporter in bovine BBB plasma membranes was 10.8 +/- 0.9 pmol/mgp (mean +/- S.E., n = 6). This value was not statistically different from the estimate of the maximal binding sites of D-glucose-displaceable [3H]cytochalasin B binding in the BBB membrane preparations, 11.7 +/- 3.5 pmol/mgp. In situ hybridization experiments using 35S-labeled antisense and sense riboprobes corresponding to nucleotides 385-932 of the GLUT-1 cDNA showed prominent hybridization of the antisense probe over brain microvascular endothelium, but no hybridization over neuropil greater than that found with the 35S-labeled sense probe. These studies are consistent with the following conclusion: (a) essentially 100% of the glucose transporter binding sites at the BBB can be accounted for by the GLUT-1 isoform; (b) in situ hybridization studies confirm previous Northern blot analysis and indicate the GLUT-1 gene is expressed selectively in microvascular endothelium in brain with minimal, if any, expression of this gene in neurons or glial cells in vivo.
Dirk Tanis, BA, MSci
Chief Operating Officer, Applied Nutriceuticals
Glucaronactolone..stuff makes me as hyper as a kid on Candy Corn.
I'm not sure of anything but it'd be interesting to try it when studying for tests and before tests to improve cognitive ability. Maybe you wouldn't get brain burn out at the end of a 3 hr calculus exam(1st year engineering suuuucks haha).