This is kind of tough to answer, because it depends on the pharmacodynamics of TUDCA, and I dont have all the info.
On one side I would ask - how bioavailable is it? If it is very bioavailable, great.
If it is not bioavailable, the second question is, why not? Is it because the liver metabolizes it and prevents it getting to where it is needed? If this is the case, then one bolus dose at a time would be better
And then I would also look at half life, which appears to be pretty long, at least if it is similar to UDCA - 3-6 days.
I dont have the info on if it is bioavailable or if it is metabolized by a liver enzyme. Liver metabolism favors larger doses because if you have enough enzyme to break down 400 mg of a substance and you take 500 mg - you will get 100 past the liver simply because you exhausted the enzyme. And then the enzyme replenishes and you take 500 and get another 100 past - but if you took 1,000 all at once, you would get 600 mg past.
Given that some studies show effects with very small doses of TUDCA, my belief is that this is not relevant.
And since the half life is so long, I suspect consistency is far more important than # of times a day that you take it. After a while the peaks and troughs will be only minimally different.