Animals, diets, and treatment
A first series of experiments was performed on 5-week-old male C57BL/6J-ob/ob mice (henceforth referred to as ob/ob mice) purchased from Janvier (Le-Genest-St-Isle, France) and fed ad libitum a normal chow (A04 biscuits; UAR, Villemoisson-sur-Orge, France). This standard-calorie (SC) diet brings 2,900 kcal/kg of food and contains 3% fat, 60% complex carbohydrate (primarily starch), and 16% protein. After a week of acclimatization in the animal house, ob/ob mice were treated with 100 or 500 mg/kg/day of BAIBA (Sigma-Aldrich, Saint-Quentin Fallavier, France) for 4 months. BAIBA was administered in the drinking water, as previously described (6,7). In ob/ob mice, but not in ob/+ mice, we rapidly noticed that BAIBA slightly but significantly lowered the daily consumption of drinking water by 15%. Consequently, the concentration of BAIBA was adjusted in the drinking solution so that the daily intake of BAIBA was kept constant.
A second series of experiments was performed on 5-week-old male C57BL/6J-+/+ mice (wild type, referred to as +/+ mice) and C57BL/6 -ob/+ mice (partially deficient in leptin, henceforth referred to as ob/+ mice) purchased from Janvier. After a week of acclimatization, both +/+ and ob/+ mice were fed ad libitum a high-fat, high-sucrose diet (referred to as high-calorie (HC) diet) purchased from SAFE (Augy, France). The HC diet brings 5,500 kcal/kg and includes 35% fat (primarily lard), 36% simple carbohydrate (mainly saccharose), and 19% protein. Ob/+ mice were treated or not treated with 100 mg/kg/day of BAIBA. Thus, three different groups of mice were studied for 4 months, namely +/+ fed the HC diet and ob/+ mice fed the HC diet, treated or not treated with BAIBA. To measure food consumption, the HC diet (which presents a pastry consistency) was manually transformed into pellets that were frozen until their utilization. Food consumption was then assessed every day during 6 consecutive weeks.
Our last experiment was performed on a few +/+ mice fed the SC or the HC diet for 4 months. Hence, three different groups of mice were studied (n = 6–8 per group), namely, +/+ mice fed the SC diet and +/+ mice fed the HC diet, treated or not treated with 100 mg/kg/day of BAIBA. All experiments were performed in agreement with national guidelines for the proper use of animals in biomedical research.
. As for which to choose, I also suggest you give Lipomorph a try. Many the added effect of the other ingredients, but BAIBA solo is a simple, clean effective supplement so it also has it's own place.
