Synergistic Supplements/Stacks Thread

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    Synergistic Supplements/Stacks Thread

    General info thread:We know based on the information provided here that certain supplements/types of supplements/stacks act synergistically and are extremely effective for their intended purposes. For example: DAA + AI (Erase/Erase Pro, Triazole, Reduce XT) - Bulk/Recomp/CutForskolin + B2 Agonists (Alphamine, AT2) - Thank you, Mr.Cooper69 - Cut/RecompArachidonic Acid + Anabeta Elite - Bulk/Recomp/Cut - Thank you, RodjaThese are just two and as more pop up in this thread I will update accordingly.Thanks

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    J Endocrinol. 2006 Sep;190(3):871-8.Click here to read Links
    The involvement of epoxygenase metabolites of arachidonic acid in cAMP-stimulated steroidogenesis and steroidogenic acute regulatory protein gene expression.
    Wang X, Shen CL, Dyson MT, Yin X, Schiffer RB, Grammas P, Stocco DM.

    Department of Neuropsychiatry, Garrison Institute on Aging, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA.

    The essential role of arachidonic acid (AA) in steroidogenesis has been previously demonstrated. The present study continues the investigation into how AA regulates steroidogenesis by examining the effects of epoxygenase-derived AA metabolites on cAMP-stimulated steroidogenic acute regulatory (StAR) gene expression and steroid hormone production in MA-10 mouse Leydig cells. The HPLC analysis of cell extracts from MA-10 cells treated with the cAMP analog dibutyryl cAMP (dbcAMP) demonstrated an increase in three epoxygenase-generated AA metabolites: 5,6-epoxyeicosatrienoic acid (EET), 8,9-EET, and 11,12-EET. Incubating MA-10 cells with each of the EETs induced a dose-dependent increase in StAR protein expression and steroid hormone production in the presence of dbcAMP. These metabolites also significantly enhanced StAR gene transcription as determined by luciferase assays of StAR promoter activity and reverse transcriptase-PCR analysis of StAR mRNA levels. While the EETs enhanced steroidogenesis, inhibiting the activity of protein kinase A (PKA) abolished the stimulatory effects of these AA metabolites on StAR expression and steroid hormone production. This study suggests that cAMP stimulation of MA-10 cells increases epoxygenase-generated AA metabolites and the co-action of these metabolites with PKA significantly increases StAR gene expression and steroid hormone production.

    J Biol Chem. 2000 Jun 30;275(26):20204-9.Click here to read Links
    The role of arachidonic acid in steroidogenesis and steroidogenic acute regulatory (StAR) gene and protein expression.
    Wang X, Walsh LP, Reinhart AJ, Stocco DM.

    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA.

    This study was conducted to examine the mechanism for arachidonic acid (AA) regulation of steroidogenic acute regulatory (StAR) protein expression and the relationship between AA and cAMP in hormone-induced steroidogenesis. Dibutyryl cyclic AMP (Bt(2)cAMP)-stimulated MA-10 Leydig cells were treated with AA and/or the phospholipase A(2) inhibitor, dexamethasone. Dexamethasone significantly reduced Bt(2)cAMP-stimulated progesterone production, StAR promoter activity, StAR mRNA, and StAR protein. The inhibitory effects of dexamethasone were reversed by the addition of 150 microm AA to MA-10 cells. In addition, MA-10 cells were treated with the lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA), the 5-lipoxygenase inhibitor, AA861, the epoxygenase inhibitor, miconazole, and the cyclooxygenase inhibitor, indomethacin. Both NDGA and AA861 inhibited progesterone production and StAR protein expression. AA861-inhibited progesterone synthesis and StAR protein were partially reversed by addition of the 5- lipoxygenase metabolite, 5(S)-hydroperoxy-(6E,8Z,11Z, 14Z)-eicosatetraenoic acid. Inhibition of epoxygenase activity inhibited progesterone production significantly, but StAR protein was only slightly reduced. Indomethacin enhanced StAR protein expression and significantly increased progesterone production. Inhibition of AA release or lipoxygenase activities did not affect protein kinase A activity, whereas inhibition of protein kinase A activity using H89 reduced Bt(2)cAMP-induced StAR protein. AA alone did not induce StAR protein expression nor steroid production. These results demonstrate the essential role of AA in steroid biosynthesis and StAR gene transcription and suggest the possible involvement of the lipoxygenase pathway in steroidogenesis. This study further indicates that AA and cAMP transduce signals from trophic hormone receptors to the nucleus through two separate pathways and act to co-regulate steroid production and StAR gene expression and indicates that both pathways are required for trophic hormone-stimulated steroidogenesis.

    Mol Cell Endocrinol. 2002 Feb 25;188(1-2):55-63.Click here to read Links
    Interaction between arachidonic acid and cAMP signaling pathways enhances steroidogenesis and StAR gene expression in MA-10 Leydig tumor cells.
    Wang XJ, Dyson MT, Mondillo C, Patrignani Z, Pignataro O, Stocco DM.

    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA.

    Previous studies have demonstrated that trophic hormone stimulation induced cyclic AMP (cAMP) formation and arachidonic acid (AA) release from phospholipids and that both these compounds were required for steroid biosynthesis and steroidogenic acute regulatory (StAR) gene expression in MA-10 mouse Leydig tumor cells. The present study further investigates the synergistic effects of the AA and cAMP interaction on steroidogenesis. To demonstrate cAMP-induced AA release, MA-10 cells were pre-loaded with 3H-AA and subsequently treated with dibutyryl cyclic AMP (dbcAMP). Stimulation with dbcAMP significantly induced AA release in MA-10 cells to a level 145.7% higher than that of controls. Lowering intracellular cAMP concentration by expressing a cAMP-phosphodiesterase significantly reduced human chorionic gonadotrophin (hCG)-induced AA release. The dbcAMP-induced AA release was inhibited significantly by the phospholipase A(2) (PLA(2)) inhibitor dexamethasone (Dex) and also by the protein kinase A (PKA) inhibitor H89, suggesting the involvement of PKA phosphorylation and/or PLA(2) activation in cAMP-induced AA release. The effect of the interaction between AA and cAMP on StAR gene expression and steroid production was also investigated. While 0.2 mM dbcAMP induced only very low levels of StAR protein, StAR mRNA, StAR promoter activity and steroid production, all of these parameters increased dramatically as AA concentration in the culture medium was increased from 0 to 200 microM. Importantly, AA was not able to induce a significant increase in steroidogenesis at any concentration when used in the absence of dbcAMP. However, when used in concert with submaximal concentrations of dbcAMP (0.05 mm to 0.5 mm), AA was capable of stimulating StAR gene expression and increasing steroid production significantly. The results from this study demonstrate that AA and cAMP act in a highly synergistic manner to increase the sensitivity of steroid production to trophic hormone stimulation and probably do so by increasing StAR gene expression.
    M.Ed. Ex Phys
    Performax Labs Product Specialist

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    Holy wall of text Batman! take home msg: ArA (such as Xgels) + forskolin (F95) to increase cAMP = sweet, sweet synergy.
    ~SNS~ Serious Nutrition Solutions- Rep
    Cortisol control:ReduceXT/Test Boost:DAA (bulk&caps)/Pump/N.O.:Agmatine(bulk & caps)/Test/sleep support:ZMA **RB** Est Post #22

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    Ara + Hemavol + Compete for the GMS and Carnitine sources along with Cit-Mal and Agmatine. Bam.
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    So many good stacks....

    Acute FX + High Volume
    Alphamine + High Volume
    Jacked + High Volume
    Craze + High Volume
    Origin+High Volume
    Titanium + DAA + Erase
    Anabeta+Xgels+High Volume
    Super Cissus + Fish Oils + Flex4Jjoints
    Origin + protocol + Creignite
    Free Test + HGH UP + Titanium
    Acute FX + Shift + 7-Keto + Green Coffee Bean
    Core Nutritionals Representative
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    Forskolin + Alphamine + Erase Pro...and it's on sale for 69.99 on NP right now
    The above is my own opinion and does not reflect the opinion of PES


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