Discussion Administration of extract resulted in weight gain in treated animals. Testes and epididymal
weights were also increased significantly, while a moderate but insignificant increase in
prostate weight was also observed. The increase in body and organ weights was
comparable with testosterone (Table 1).
Since androgenic effect is attributable to
testosterone levels in the blood, it is likely that the plant extracts may have a role in
testosterone secretion allowing better availability of hormone to gonads . A significant
anabolic effect upon administration of extracts was observable as compared to the control
group, which was comparable to that of the administration of testosterone suggesting a
testosterone type action of the extracts. The experiment clearly suggests anabolic
steroidal effect of A. pyrethrum.
The enhancement of sexual activity has been directly
correlated to the enhancement of sexual pleasure. Penile erection index is important for
evaluating the effect of administration of extract on erectile function . The aqueous
extract of A. pyrethrum increased the penile erection of male rats, similar to the increase in
penile erection index observed by administration of testosterone. An increase in PEI was
observed in treated groups, indicating the involvement of nitrous oxide (NO) based
.The effect in A. pyrethrum aqueous extract treated group (100 mg/Kg) is
much more pronounced when compared to the administration of testosterone.
Administration of extract (50 and100 mg/Kg b. w.) modified both the orientation as well as
sexual behavior, conclusively suggesting a better sexual performance after administration 108 V. Sharma et al.:
Sci Pharm. 2009; 77; 97–110.
of the extracts. This activity was also validated by the histopathological studies on testis
section of various groups. The results thus confirm that, the drug extracts can be useful in
enhancement of overall sexual performance of male rats. Drug also shows prolonged and
sustained effect in overall sexual performance conducted during this study.
may be affected directly by increasing serum testosterone level or by having testosterone
like effect. This may be due to the change in neurotransmitter level or their action in the
cell could also changed sexual behavior [7, 20]. Spermatogenesis involves a complex
interplay between the structural element of testis and the endocrine system .
FSH stimulates spermatogenesis and LH stimulates synthesis and release of testosterone.
Testosterone causes an increased blood flow and stimulates the growth of the target
tissues. Testosterone cause direct stimulation of spermatogenesis. Our results also show
that there is increase in spermatogenesis and increase in weight of sexual organ in
extracted treated group as comparison to control group .
The improvement of in-vivo
sperm count suggests an improved spermatogenic activity of the test extract. Similarly, the
seminal fructose count improvement also indicates that the aqueous extract of A.
pyrethrum enhances the quality of seminal parameters which eventually assist in better
reproductive potential. Preservation of in-vitro sperm count is an indicative that the herb
contains water soluble nutritive substances that help sperms in avoiding death due to lack
of energy. One the other hand the fructose content is also regulated by androgens and
fructose content establishes a narrow relationship among the levels of testosterone and
fructose production by the seminal vesicle . Therefore, the results corroborate the hype
that the plant is capable of being nominated as herbal alternative for improving sexual
function. Since, the herb had a beneficial effect on various physiological conditions
responsible for exhibiting a better sexual performance i.e. improved anabolic activity,
better orientation activities, improvement in seminal fructose level and an overall
improvement in various sexual behavior parameters. Hence, these findings provide a
scientific basis for acclaiming A. pyrethrum as ‘Vajikarana Rasayana’.