ROS, Antioxidants and muscle hypertrophy

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    ROS, Antioxidants and muscle hypertrophy


    Acta PhysiologicaVolume 208, Issue 1,
    A role for reactive oxygen species in the regulation of skeletal muscle hypertrophy


    It is clear that at pathologically high chronic levels,
    reactive oxygen species (ROS) are cytotoxic. However,

    it is also now clear that during contraction ROS are
    produced at low (physiological) levels and play an
    important role in cell signalling in normal healthy
    skeletal muscle (reviewed in Powers & Jackson 2008).
    To date, much of the work regarding skeletal muscle
    ROS produced during contraction has focussed on
    endurance exercise. This has led to considerable con-
    troversy in the literature regarding the potential for
    antioxidant supplements to prevent skeletal muscle
    adaptations to endurance training, such as increased
    mitochondrial biogenesis, with some studies support-
    ing such a role (Gomez-Cabrera et al. 2008, Ristow
    et al. 2009), whilst others do not (Yfanti et al. 2010,

    Higashida et al. 2011, Strobel et al. 2011).

    Hypertrophy is a skeletal muscle adaptation follow-
    ing a period of muscle overload, for example, follow-
    ing resistance exercise training. Makanae et al. (2013)

    now suggest a role for ROS as a regulator of skeletal
    muscle hypertrophy following muscle overload. The
    authors used the well-established rodent model of
    mechanically overloading the plantaris by surgically
    removing the synergistic gastrocnemius and soleus
    muscles of the hindlimb. As expected, two weeks
    following surgery, there was a significant increase in
    skeletal muscle hypertrophy. Makanae et al. (2013)

    also found that a high daily oral dose of the antioxi-
    dant vitamin C, attenuated skeletal muscle hypertro-
    phy and oxidative stress, as measured by the ratio of
    oxidized to total glutathione. The findings of Makanae
    et al. are topical, because other very recent findings (Ito

    et al. 2013) demon-
    strate that the highly reactive oxidant, peroxynitrite,

    which is formed by superoxide with nitric oxide, regu-
    lates skeletal muscle hypertrophy induced by overload.
    Another interesting finding by Makanae et al.

    (2013) was that endogenous levels of skeletal muscle
    vitamin C were increased in the placebo group follow-
    ing overload, although not to the same extent as the
    vitamin C-treated animals. This is likely a compensa-
    tory increase in antioxidant defences within skeletal
    muscle of the placebo group in response to the oxida-
    tive stress. Indeed, levels of glutathione, one of the
    major nonenzymatic antioxidants in skeletal muscle
    (Powers & Jackson 2008), also significantly increased
    in response to overload (Makanae et al. 2013). There-

    fore, the increased skeletal muscle vitamin C levels in
    the placebo group highlight, as the authors point out, a
    limitation with the roden t model, because unlike

    humans, rats are able to synthesize their own vitamin C.
    Thus, the upregulation of skeletal muscle vitamin C
    levels in the placebo-treated group following surgery
    could also potentially explain why exogenous
    treatment of vitamin C only mildly attenuated the
    skeletal muscle hypertrophy.
    As mentioned earlier, considerable controversy
    exists as to whether high dose antioxidant supplemen-
    tation prevents skeletal muscle adaptations following
    endurance training. However, the few human studies
    to investigate resistance training with antioxidants
    have shown no effect of vitamin C and E supplemen-
    tation on skeletal muscle adaptations (Bobeuf et al.

    2011, Theodorou et al. 2011), although this is not

    sufficient evidence in itself to exclude a role for ROS
    in the regulation of human skeletal muscle hypertrophy.
    Furthermore, these rodent studies (Ito
    et al. 2013, Makanae et al. 2013) are still quite relevant to

    humans, since understanding the molecular pathways
    that regulate muscle mass may provide important
    therapeutic targets for people with muscle wasting
    conditions.
    Reactive oxygen species and skeletal muscle adaptation to exercise

    Physical exercise promotes signalling responses in skeletal muscle leading to alterations in protein synthesis and muscle phenotype. For example, as few as five consecutive days of endurance exercise (i.e. 60 min day−1 at 60% of maximal oxygen uptake) promotes an increase in both mitochondrial enzymes and antioxidants in the active skeletal muscles (Vincent et al. 2000). The fact that ROS are generated in contracting muscles and many signalling pathways are redox sensitive has raised the question of whether contraction-induced ROS production plays an essential role in skeletal muscle adaptation to aerobic exercise training. The short answer to this question is yes, and evidence to support this position follows.

    Davies and colleagues provided the first suggestion that ROS production is a stimulus for skeletal muscle adaptation to exercise training (Davies et al. 1982). Since this initial proposal, many studies have supported this concept. For example, abundant in vitro studies have demonstrated that exposure of cultured myotubes to oxidants (e.g. hydrogen peroxide) promotes the expression of numerous genes (Li et al. 2003; McArdle et al. 2004; Hansen et al. 2007; Irrcher et al. 2009; McClung et al. 2009). Specifically, hydrogen peroxide exposure has been shown to augment the expression of key antioxidant enzymes in myotubes (Franco et al. 1999). Moreover, a recent study reveals that exposure of myotubes to exogenous hydrogen peroxide increases peroxisome proliferator-activated receptor-γ coactivator-1 protein-α (PGC-1α) promoter activity and mRNA expression (Irrcher et al. 2009). Importantly, both effects were blocked when the antioxidant N-acetylcysteine was added to the culture medium. It has also been reported that ROS production is a requirement for contraction-induced gene expression of PGC-1α in primary rat muscle cells (Silveira et al. 2006). Collectively, these in vitro experiments demonstrate that ROS are capable of altering gene expression in cultured muscle cells.

    Similar to the aforementioned cell culture studies, numerous reports support the concept that exercise-induced ROS production alters muscle gene expression and contributes to exercise-induced adaptations of skeletal muscle in vivo. A common approach in many of these studies is to abolish the signalling effects of exercise-induced ROS production in skeletal muscle by treating animals or humans with antioxidants. For example, two independent reports have demonstrated that exercise-induced expression of heat shock protein 72 in rat skeletal and cardiac muscle is suppressed by antioxidant supplementation (Hamilton et al. 2003; Jackson et al. 2004). Also, two independent studies have recently concluded that antioxidant supplementation can retard important training adaptations in human skeletal muscle (Gomez-Cabrera et al. 2008; Ristow et al. 2009). Specifically, Gomez-Cabrera et al. (2008) reported that oral administration of vitamin C in humans prevented exercise-induced expression of PGC-1α and mitochondrial biogenesis in skeletal muscle. Furthermore, vitamin C treatment also prevented exercise-induced expression of several antioxidant enzymes in muscle (Gomez-Cabrera et al. 2008). Similar results in humans have been reported by Ristow et al. (2009). Together, these findings support the conclusion that ROS production plays an essential role in exercise-induced skeletal muscle adaptation.

    //onlinelibrary.wiley.com/doi/10.1113/expphysiol.2009.050526/full

    Maybe its time to shelf NAC..

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    Supplementation with vitamin C and N-acetyl-cysteine increases oxidative stress in humans after an acute muscle injury induced by eccentric exercise


    Abstract

    There has been no investigation to determine if the widely used over-the-counter, water-soluble antioxidants vitamin C and N-acetyl-cysteine (NAC) could act as pro-oxidants in humans during inflammatory conditions. We induced an acute-phase inflammatory response by an eccentric arm muscle injury. The inflammation was characterized by edema, swelling, pain, and increases in plasma inflammatory indicators, myeloperoxidase and interleukin-6. Immediately following the injury, subjects consumed a placebo or vitamin C (12.5 mg/kg body weight) and NAC (10 mg/kg body weight) for 7 d. The resulting muscle injury caused increased levels of serum bleomycin-detectable iron and the amount of iron was higher in the vitamin C and NAC group. The concentrations of lactate dehydrogenase (LDH), creatine kinase (CK), and myoglobin were significantly elevated 2, 3, and 4 d postinjury and returned to baseline levels by day 7. In addition, LDH and CK activities were elevated to a greater extent in the vitamin C and NAC group. Levels of markers for oxidative stress (lipid hydroperoxides and 8-iso prostaglandin F; 8-Iso-PGF) and antioxidant enzyme activities were also elevated post-injury. The subjects receiving vitamin C and NAC had higher levels of lipid hydroperoxides and 8-Iso-PGF 2 d after the exercise. This acute human inflammatory model strongly suggests that vitamin C and NAC supplementation immediately post-injury, transiently increases tissue damage and oxidative stress.


    On the contrary, in vitro examples weigh more
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    I have seen these before and I think that the gist is to not supplement your antioxidants around your workouts as that could inhibit hypertrophy. But antioxidants still can have a place in supplementation. In for some thoughts on this.

    Definitely interesting stuff.
    http://anabolicminds.com/forum/workout-logs/231713-rob112-3-means.html
    "Train like a beast, think like a human"-RTS
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    Excellent thread
    http://pescience.com/
    http://selectprotein.com/
    The above is my own opinion and does not reflect the opinion of PES
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    Quote Originally Posted by rob112 View Post
    I have seen these before and I think that the gist is to not supplement your antioxidants around your workouts as that could inhibit hypertrophy. But antioxidants still can have a place in supplementation. In for some thoughts on this.

    Definitely interesting stuff.
    Im not quite sure about that. Evidence suggests that oxidative stress markers were elevated up to 48h post exercise. This was checked by measuring antioxidative enzyme expression. Although this may be just a prolonged compensatory mechanism from acute oxidative stress during exercise, its impossible to tell. Antioxidants still have their place, but overconsumption of them may not.

    Also, this paper suggests an anabolic effect of increased NO levels during exercise through formation of peroxynitrite radical. Turns out NO isnt strictly cosmetic at all.

    Here we show that neuronal nitric oxide synthase (nNOS) regulates load-induced hypertrophy by activating transient receptor potential cation channel, subfamily V, member 1 (TRPV1). The overload-induced hypertrophy was prevented in nNOS-null mice. nNOS was transiently activated within 3 min after overload. This activation promoted formation of peroxynitrite, a reaction product of nitric oxide with superoxide3, which was derived from NADPH oxidase 4 (Nox4). Nitric oxide and peroxynitrite then activated Trpv1, resulting in an increase of intracellular Ca2+ concentration ([Ca2+]i) that subsequently triggered activation of mammalian target of rapamycin (mTOR). Notably, administration of the TRPV1 agonist capsaicin induced hypertrophy without overload and alleviated unloading- or denervation-induced atrophy. These findings identify nitric oxide, peroxynitrite and [Ca2+]i as the crucial mediators that convert a mechanical load into an intracellular signaling pathway and lead us to suggest that TRPV1 could be a new therapeutic target for treating muscle atrophy.
    nature.com/nm/journal/v19/n1/full/nm.3019.html
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    Ok, for business-minded non-study readers...is this correct?

    1) ROS cause tissue damage and inflammation which signal increased hypertrophy.

    2) Vitamin C and NAC reduce ROS and therefore tissue damage and inflammation and therefore the anabolic response to eccentric training.

    3) Vitamin C and NAC increase tissue damage and inflammation in response to acute injury, and this is bad instead of good?

    4) So, Vitamin C and NAC are bad when you want a little inflammation after training because they mitigate it, and they are also bad when you'd want to limit out of control inflammation during a serious injury because they exacerbate/prolong it??? Vitamin C and NAC are bad in any conceivable situation?

    5) What is the difference between Vitamin C and NAC and something like Pomegranate Fruit Extract that I'd find in my Enhanced?

    6) What is the difference between these two and something with antioxidant properties of Agmatine?
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    I wouldnt worry about the antioxidative properties of polyphenols and other phytochemicals. According to the FDA there is no proof that any of them possess any antioxidant activities in vivo. Overconsumption of certain vitamins and possibly NAC since its a precursor to glutathione is what you should watch out for.
  

  
 

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