pestis
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Does anyone know how much (if any) fina is lost in the whatman filter during a conversion?? I have tried to find an answer to this for some time but have had no luck. anyone???
I would say you might be a bit wrong on that.. the size of the molecule of tren would NOT be filtered out by a .22 or a .45 whatman.. just can't happen.. using just the coffee filter will run your risk of a nice infection though.. and if you would like I can post you some nice pictures of infections caused by injection of substances that were contaminated by normal skin bacteria
Good info D. Strep ain't nothin' nice either. Necrotizing fasciitis has almost taken me out twice over the years. Always filter. With tren from pellets, double filter at a later date helps too, and always have a fast acting, broad spectrum antibiotic on hand!... Your skin has two type of staph that normally live there and all it takes is a nice jab and away they go.. bottom line, not bacteria no infection, trust me on this one
Skin abcess link
http://www.nlm.nih.gov/medlineplus/ency/article/000863.htm
well as most of my experance comes from vet work (just working, not a vet) I will take your word for it. But it was my understanding that it still takes a minute or two for everthing to die and most people do not wait that long (or this not the whole story here? the guy I worked with was a 67 year old man but he was very good)I am actually getting from the classes I teach on microbiology skye... there are two types of abcesses.. alcohol will not kill most bacteria that are found on the skin just move them around. If you really want to kill them use hydrogen peroxide. Your skin has two type of staph that normally live there and all it takes is a nice jab and away they go.. bottom line, not bacteria no infection, trust me on this one
Skin abcess link
http://www.nlm.nih.gov/medlineplus/ency/article/000863.htm
Just use the crystal fina methoed.Good info D. Strep ain't nothin' nice either. Necrotizing fasciitis has almost taken me out twice over the years. Always filter. With tren from pellets, double filter at a later date helps too, and always have a fast acting, broad spectrum antibiotic on hand!
Any part of the process that involves a whatman filter. In particular, each time I use a partial vial that has been sitting around a few months I always refilter with a .45 just to be safe. I was just wondering how much fina would be lost each time filtering is done. Sounds like the answer is none...This question needs to be more specific; I can see some confusion arising in this thread due to ambiguity. Are you referring to the syringe filter when 'sterilizing' the final product, or are you referring to the filtration of the methyl cellulose binders from the fina pellets? Remeber, Whatman has a very large selection of many types of filters.
I think he's talking about syringe filters, Klause. I've found that a Whatman 0.45 PTFE hydrophob or 0.7mic works great the first time, then run through a sterile Whatman 0.2 PTFE/GMF. Gelman's are sometimes faster but not as efficient it seems (makes sense). Heat sterilization can be avoided if you can follow the other rules I think (sterile final filter and at least 2% BA with the high% BB pres).This question needs to be more specific; I can see some confusion arising in this thread due to ambiguity. Are you referring to the syringe filter when 'sterilizing' the final product, or are you referring to the filtration of the methyl cellulose binders from the fina pellets? Remeber, Whatman has a very large selection of many types of filters.
The loss is low, less than 1 ml is trapped in the filter, so even if you don't flush it, you lose less than 100mg with each filtration (assuming you cooked 100mg/ml concentration).... I was just wondering how much fina would be lost each time filtering is done. Sounds like the answer is none...
makes sense. thanks DThe loss is low, less than 1 ml is trapped in the filter, so even if you don't flush it, you lose less than 100mg with each filtration (assuming you cooked 100mg/ml concentration).
That's what I do too! Just be careful at the end to stop before you push the air all the way though into you stuff. A small amount of loss is OK.I figured out last night, you can just pull the syringe off, load it with air, and push all of the oil through without purging with oil
That's what I did anyway, haha. I didn't wanna screw up the concentration b/c it was a small batch, so I did that.
I have another "venting" needle in there anyway, so excess air was no biggyThat's what I do too! Just be careful at the end to stop before you push the air all the way though into you stuff. A small amount of loss is OK.
I'm brewing for a while and never got an infection.I am actually getting from the classes I teach on microbiology skye... there are two types of abcesses.. alcohol will not kill most bacteria that are found on the skin just move them around. If you really want to kill them use hydrogen peroxide. Your skin has two type of staph that normally live there and all it takes is a nice jab and away they go.. bottom line, not bacteria no infection, trust me on this one
Skin abcess link
http://www.nlm.nih.gov/medlineplus/ency/article/000863.htm
I've had particles in the vail that came from the rubber stopper breaking apart from the needle pushing through. I just pulled them out with an 18g.I'm brewing for a while and never got an infection.
Altough I changed my vials supplier, and I made two vials one of prop and another or tren ace! both of them have been double filtered (.45 and later .22) and the other day, i just, ocassionally put one of the vials trough the light and noticed that there were some small particules floating, and check the tren and it had them too altough they were doble filtered and went to baking one hour at 135ºC/275ºF.
I think it migh be dust that were on the vials, but they were supposedlly sterile when i bough them...
I've hunted them down, but I'm not very confident! I've tryed one a week ago of the bottles, and no problems but i dunno if i inject that particle!
Should I worry about this stuff?
sorry to hijack the thread, but since you guys were talking about infections...