Jiigzz
Legend
- Awards
- 5
This has come up a fair amount recently and a few people are still unsure of the concept. Whenever a new drug is trialed, it almost always goes through some sort of animal testing before being allowed to enter the market for human use.
Unfortunately it is often presumed that to convert the dose used from a mouse to a human, is to simply multiply the amount a mouse was given by the weight of a human. This is simply not the case (as detailed below). This is an exert from:
Fasebj | Mobile
CORRECT DOSE CALCULATION: AN EXAMPLE
As described above, confusion and concerns emanated from a recent study by Baur*et al.(2)⇓*, where a dose of 22.4 mg/kg (body weight) of resveratrol was used in a mouse study on aging and obesity-related disorders. The media reported that a 60 kg human would have to consume 1344 mg of resveratrol per day in order to receive a like benefit, a serious misinterpretation of the research. Using an average of 2 mg resveratrol per bottle of wine (6)⇓*, this calculation implies that a person would have to drink 672 bottles of red wine to approximate the resveratrol equivalent.
However, the Food and Drug Administration (7)⇓*has suggested that the extrapolation of animal dose to human dose is correctly performed only through normalization to BSA, which often is represented in mg/m2. The human dose equivalent can be more appropriately calculated by using the formula shown in*Fig. 1⇓*. To convert the dose used in a mouse to a dose based on surface area for humans, multiply 22.4 mg/kg (Baur’s mouse dose) by the*Km*factor (3) for a mouse and then divide by the*Km*factor (37) for a human (Table 1⇓*). This calculation results in a human equivalent dose for resveratrol of 1.82 mg/kg, which equates to a 109 mg dose of resveratrol for a 60 kg person.*
I hope this is useful to you all!
Unfortunately it is often presumed that to convert the dose used from a mouse to a human, is to simply multiply the amount a mouse was given by the weight of a human. This is simply not the case (as detailed below). This is an exert from:
Fasebj | Mobile
CORRECT DOSE CALCULATION: AN EXAMPLE
As described above, confusion and concerns emanated from a recent study by Baur*et al.(2)⇓*, where a dose of 22.4 mg/kg (body weight) of resveratrol was used in a mouse study on aging and obesity-related disorders. The media reported that a 60 kg human would have to consume 1344 mg of resveratrol per day in order to receive a like benefit, a serious misinterpretation of the research. Using an average of 2 mg resveratrol per bottle of wine (6)⇓*, this calculation implies that a person would have to drink 672 bottles of red wine to approximate the resveratrol equivalent.
However, the Food and Drug Administration (7)⇓*has suggested that the extrapolation of animal dose to human dose is correctly performed only through normalization to BSA, which often is represented in mg/m2. The human dose equivalent can be more appropriately calculated by using the formula shown in*Fig. 1⇓*. To convert the dose used in a mouse to a dose based on surface area for humans, multiply 22.4 mg/kg (Baur’s mouse dose) by the*Km*factor (3) for a mouse and then divide by the*Km*factor (37) for a human (Table 1⇓*). This calculation results in a human equivalent dose for resveratrol of 1.82 mg/kg, which equates to a 109 mg dose of resveratrol for a 60 kg person.*
I hope this is useful to you all!
