First homebrew baking process Question
- 01-29-2005, 06:03 PM
First homebrew baking process Question
Alright I just made up some 1-test cyp....everything was working fine..
Until I stuck this into my oven at 250 and walked away for 2 minutes.
I come back and the plastic on my needle is melting like the Wicked Witch of the West man. WTF? Obviously I had to abort the operation, as the melted plastic sealed up the hole in the needle.
I'm thinking because I own a convection oven maybe the temperature was too high? I dont want to turn the temperature down too much however, has anyone else had this problem?
Any help is appreciated.
- 01-29-2005, 07:02 PM
If you must bake (personal preference I guess), cut of the top off the plastic with some wire cutters. There will be a little plastic left, but not enough to cover the hole in the pin when it melts.
01-29-2005, 07:11 PM
So baking isn't necessary?
I was just following instructions from another thread and wanted to make sure I got it right.
01-29-2005, 07:46 PM
As long as your are using BA at any percentage above .9%, bacteria cannot grow. However, some prefer to bake. I typically do it just to play it safe. It is best to bake and then cool several times, as it is the heating and cooling that kills bacteria.Originally Posted by mmorpheuss
01-30-2005, 03:58 AM
Thanks. I cut the excess plastic off and baked it up twice so far no problemOriginally Posted by bow
01-30-2005, 01:16 PM
Glad to hear it worked out.Originally Posted by mmorpheuss
BTW, for those interested. When I say cooling, the best way to do this is to cool in a frig. It is the rapid heating and then cooling that does the bacteria in.
01-30-2005, 02:44 PM
You can also just use a needle to suck the air out a few times, creating a vacuum in the vial. Then there is no risk of pressure buildup. Also this reduces oxidation--evident by the solution turning reddish if you are making tren.
Last edited by Strateg0s; 01-30-2005 at 03:08 PM.
01-30-2005, 02:54 PM
Interesting. Makes sense. Any rules of thumb with regards to the amount of air you draw per vial size prior to heating (ml/ml).
01-30-2005, 03:07 PM
Just keep plunging back in and drawing more out until it is obvious there is a vacuum. Using something bigger than 3cc would be a good idea, because then you may only have to pierce the septum once. When you pull up the plunger and it snaps back if you let it go, you've got a pretty decent vacuum.
01-30-2005, 03:08 PM
01-30-2005, 03:26 PM
Personally, from what I know about microbiology, I would take the extra step. It should not hurt anything except some of the bacteria the can live in alcohol like some of the members of the Archaea domain but I am overly cautious anyway. IF you could get you hands on an autoclave you would have the best possible setup...
02-05-2005, 07:39 PM
Baking to create a sterile product is unnecessary. If your oven was hot enough to melt the plastic on your pin, then it could be hot enough to cook/burn the oil. I've seen it in the past. If your oil becomes foggy after cooling after you have baked it, the the oil is cooked. Pushing your chemicals through a 0.45 micron filter is enough to catch bacteria... and whatever the filter might not catch, the alcohol certainly will. Good luck.
02-06-2005, 02:44 AM
sorry i disagree...Originally Posted by MassLabKits
the filters cannot catch all bacterias, and yes even the .20. even whatman will tell you that, its in their website.
02-06-2005, 10:22 AM
Umm, you failed to see this part of my response Kojie:Originally Posted by kojie
"... and whatever the filter might not catch, the alcohol certainly will. Good luck."
02-06-2005, 11:04 AM
Originally Posted by MassLabKits
Yes, but then again, it depends on the % of BA used. If enough BA is used, I tend to agree.
02-06-2005, 09:20 PM
02-06-2005, 10:43 PM
I agree... BA stops the propagation/growth of bacteria but won't necessarily kill all of it. I would play it safe and always bake. What's the big deal with spending an hour or so to bake?? A small price to ensure sterility.Originally Posted by kojie
I've baked alot of gear in the past and have never seen the plastic on the needle melt. I always bake at 225-250 deg F for 15 minutes and rapidly cool 3 times. I also use a thermometer to make sure the oven temp is accurate. If the plastic on your needle melted, i believe that the temp must have been way higher than 250 deg.
02-07-2005, 04:24 AM
"gram negative bacteria benzyl alcohol"
try searching google for that there are hundreds of articles about it
01-29-2007, 07:18 PM
01-29-2007, 07:30 PM
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