Do they have to be sterile? GPZ is out of 0.45 micron sterile and researchkits has been having trouble filling orders lately. I can get non-sterile at GPZ. If anyone knows another place that is reliable let me know. Thanks
They are among the best. Great service and resonable prices.I went ahead and ordered from gearbox. There are a SM sponser. Anyone used them?
I've got about 30 .45um sterile Whatmans left; in talks w/ a mod to see how to approach selling these, among other things. E-mail me securely at [email protected] for pricing. I've got some hi-flow .22um & .45um syringe filters a mod's trying, so if they're any good, I'm sure he'll mention them.Do they have to be sterile? GPZ is out of 0.45 micron sterile and researchkits has been having trouble filling orders lately. I can get non-sterile at GPZ. If anyone knows another place that is reliable let me know. Thanks
LMAO!! That's why I haven't carried them in the first place, but now, I'm thinking pre-filter, then .22um filter to finish up. All my hi-cap. vacuum filters are .2um PVDF for sterility reasons, and can be used w/ pre-filters for longer primary filter life. They will be tested and approved by a mod first.all I know is that these .22's better be "high flow", because using regular one's seems virtually impossible for me. When I used them my hand cramped up (from filtering) more than when I watch a Jenna Jameson flick
A clean room takes care of most of it. A .22 and BA takes care of it for us. rember this is largely a game of numbers, IE the probability of getting a infection. it is never 0. Nor does any process get rid of everthing.After reading candle25's thread www.anabolicminds.com/forum/showthread.php?t=13012 I am worried about .45um not being sufficient for others to sterilize their product w/o fully removing spores. I called to place an order w/ Whatman to order as much remaining stock as I can afford on Whatman 25mm, .22um & .45um sterile filters (which has been discontinued; I will find out the true extent of this tomorrow morn, and post results). I called after hours, so I will find out tomorrow the story behind this "discontinuance".
Does anyone know what is involved in killing spores? Does gamma irradiation (the industry standard for sterilization of the filtering products we purchase)? Or are they able to "get away with it" because they assemble and sterilize in a class "X" clean room? Thanks for the input.
I'm waiting on my 0.20um filters right now. I have some gear that has been filtered through a 0.45um filter, but the damn vile cap has a hole in it that wont close up. I'm going to filter it through a 0.20um to a new vile. I'll let you all know how it goes.I'm thinking pre-filter, then .22um filter to finish up. All my hi-cap. vacuum filters are .2um PVDF for sterility reasons, and can be used w/ pre-filters for longer primary filter life. They will be tested and approved by a mod first.
Unfortunately, filtering would have to be done in 2 draws; nobody makes a syringe pre-filter I can find w/ a locking luer outlet (I don't know if that would work well anyway). However, filtering would be considerably easier; if that isn't good enough, I've got vacuum filtering units tailored for this size run that eliminates the hassles of syringe filtering. Will be tested, and mods will report results.Is the filtering and pre-filtering done with one push? or do you have to force it through one and then draw it up again to force it through again?
ManBeast
Are you talking about those hand pump Buchner funnel type set ups? It seems like you'd lose a good bit of your gear in the funnel itself if that is what you're talking about. Plus you'd lose some more when you transfer the soln from the vacume flask to the sterile vile. I'm thinking 1-1.5 ml loss. It would be nice to have an automated system but doing it by hand isn't so hard.Unfortunately, filtering would have to be done in 2 draws; nobody makes a syringe pre-filter I can find w/ a locking luer outlet (I don't know if that would work well anyway). However, filtering would be considerably easier; if that isn't good enough, I've got vacuum filtering units tailored for this size run that eliminates the hassles of syringe filtering. Will be tested, and mods will report results.
No, these are completely different (pics below)Are you talking about those hand pump Buchner funnel type set ups? It seems like you'd lose a good bit of your gear in the funnel itself if that is what you're talking about. Plus you'd lose some more when you transfer the soln from the vacume flask to the sterile vile. I'm thinking 1-1.5 ml loss. It would be nice to have an automated system but doing it by hand isn't so hard.
I personally use a hospital aspirator which pulls 22"HG (200torr), but I've used it w/ a hand pump (brake bleeder pump works), takes a squeeze down every 5-10 minutes to maintain vacuum. My hi-vac pump works extremely well with this; however, I do such bulk that I have switched over to 142mm filter setup through a SS pressure vessel. The 500ml filter setup will do 1 gallon (4 liters) of raw (high-viscosity) chems before I have to change it (may need to use a space heater to keep filter & solutions warm); thinner solvents you can do more.what kind of vacuum are you using bro to filter bro?
If you're talking about an auto syringe dispenser, VERY nice, but you'll have to sell your car for that one ($1250-$2500; one can dream, though); well, not quite, but nice device nonethelessOk, I just finished the transfer I had mentioned earlier. My boy SethL & I took turns pushing the gear through a 0.20um filter. It was only 50ml but it was hard as hell!!! Just wanted to let you all know. The auto idea is sounding better all the time. I found them in my Fisher catalouge today. Kinda pricy IMO.
Ebay has an automated syringe dispenser/pump http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=3806978934&category=26236; bids start at $275. Sounds expensive, but not (think hand cramps and blisters), has 5 days left.If you're talking about an auto syringe dispenser, VERY nice, but you'll have to sell your car for that one ($1250-$2500; one can dream, though); well, not quite, but nice device nonetheless
The luer-lock output version of the hi-cap 10cm2 syringe filters allows you to pre-filter through a .45um, then filter through a .22um -- in one draw. It still takes about the same amount of time as pushing it through a whatman .45um 25mm filter. Just lock the .45 to the syringe, then lock the .22 to the .45 luer-lock output, and finally, lock a needle to the .22 output.Unfortunately, filtering would have to be done in 2 draws; nobody makes a syringe pre-filter I can find w/ a locking luer outlet (I don't know if that would work well anyway). However, filtering would be considerably easier; if that isn't good enough, I've got vacuum filtering units tailored for this size run that eliminates the hassles of syringe filtering. Will be tested, and mods will report results.
Forget about the back-to-back filtration (too long, complicated, and expensive). Instead, the prefilters allow efficient use, and the .22um's do 150-200 of prefiltered fluids 3-4X faster than the whatmans.The luer-lock output version of the hi-cap 10cm2 syringe filters allows you to pre-filter through a .45um, then filter through a .22um -- in one draw. It still takes about the same amount of time as pushing it through a whatman .45um 25mm filter. Just lock the .45 to the syringe, then lock the .22 to the .45 luer-lock output, and finally, lock a needle to the .22 output.
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