[datBtrue]

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[datBtrue]

Originally Posted by longrob

Thanks for clearing that up Dat. Let me ask, would 100mcg on Monday and again on Thursday simply be too little CJC-1295? That was more what I had tried to say.

Sure it will help. Especially if you use inexpensive GHRP-6 throughout the week.

You will have a well supported night GH pulse on Monday & Thursday (especially if you dosed a spot of GHRP-6) and deep sleep. On the other days the GHRP-6 by itself will make use of the declining GHRH (CJC-1295) levels floating around.

CJC-1295 levels for the week will be:

Monday: 100mcg
Tuesday: 90mcg
Wednesday: 81mcg
Thursday: 72mcg + 100mcg = 172mcg
Friday: 65mcg + 90mcg = 155mcg
Saturday: 59mcg + 81mcg = 140mcg
Sunday: 53mcg + 72mcg = 125mcg

I'd like to see someone low dose CJC-1295 while using GHRP-6. Especially someone in the age bracket of 40 or above ...because now that I have been on several different dosing levels I feel that maybe even the 100mcg of CJC-1295 per day might result in more GH than what is needed for a youthful restoration.

The 2.1 mg per week of CJC-1295 is surely turning out to be a substantial bodybuilding dose in my 42 year old body.

[moose069]

D,

Did you reconstitute CJC-1295 with NACL? Subq or IM?

What about the GHRP-6?

[datBtrue]

Originally Posted by moose069

Did you reconstitute CJC-1295 with NACL? Subq or IM?
What about the GHRP-6?

Subcutaneous is the easiest but intramuscular is fine as well. BUT there is no local benefit to injecting into muscle.

By local benefit I am simply referring to either a local decrease in protein degradation or increase in protein synthesis. This has been shown to occur with insulin, growth hormone and IGF-1.

The reason is simply that these hormone/peptides (CJC-1295 & GHRP-6) are not end-product hormones. Therefore they really are not capable of acting locally. There actions will subsequently result in end product hormones.

The reconstitution agent for both CJC-1295 & GHRP-6 is Bacteriostatic Water (BW).

Here are a few interesting snippets from studies on storage:

Storage

The following paragraph comes from a study that sent the peptide home with study participants for self-administration.

Vials were stored frozen until dispensed to the subjects, then kept at 4 C for 10 days at home. High performance liquid chromatographic analysis showed that the peptide was stable at 4 C for at least 2 weeks.

The following seems to indicate that GHRP-6 which is a simple peptide chain unlike IGF-1 which is more complex, is relatively resistant to degradation under the right circumstances at room temperature for almost five years.

The influence of the various buffer species (acetate, citrate, phosphate and borate) was shown to be different and the maximum stability of GHRP-6 was revealed to be in acetate buffer of pH 5.5-6.0. Degradation of GHRP-6 was greater in citrate-containing buffers than in acetate-containing ones. Furthermore, in the citrate-containing buffers, the higher buffer concentration caused greater degradation than the lower ones, but the concentration effect was negligible in acetate-containing buffers. Aqueous solution of GHRP-6 buffered with acetate (0.01 M, pH 5.5) showed a predicted t90% of 4.73 years at 20°C. - Degradation kinetics of growth hormone-releasing hexapeptide (GHRP-6) in aqueous solution, In Sik Ha… International Journal of Pharmaceutics Volume 144, Issue 1, 22 November 1996, Pages 91-97

It is worth noting the characteristics of the primary aqueous solution we use to reconstitute these peptides:

Bacteriostatic Water for injection, USP is a sterile, nonpyrogenic preparation of water for injection containing 0.9% (9 mg/mL) of benzyl alcohol added as a bacteriostatic preservative. It is supplied in a multiple-dose container from which repeated withdrawals may be made to dilute or dissolve drugs for injection. The pH is 5.7 (4.5 to 7.0)

[moose069]

The CJC-1295 supplier shipped the peptide with a bottle of NACL and notes on their website the following:

"Kit includes necessary sterile solvent"

I wonder why they provide NACL?

Is there any advantage / disadvantage in terms of CJC-1295 shelf life (in the fridge) when reconstituted with NACL?

[moose069]

I should add, it is specifically 0.9% Sodium Chloride

[longrob]

dat,
Thanks for the advice, I appreciate it. Hopefully, I will not find the cost to be an obstacle in the future.

[datBtrue]

Originally Posted by moose069

The CJC-1295 supplier shipped the peptide with a bottle of NACL and notes on their website the following:

"Kit includes necessary sterile solvent"

I wonder why they provide NACL?

Is there any advantage / disadvantage in terms of CJC-1295 shelf life (in the fridge) when reconstituted with NACL?

The average PH is slightly lower but that is of little consequence. There is no worry about the peptide...the worry is with the end user.

Is there any bacteria in the vial? How do you know it is sterile...because "they" said so?

How about the lypholized powder is it sterile or is there bacteria in it? How do you know it is sterile...was it made in a GMP facility?

How about the salt water? Is that sterile?

What if a little bacteria makes its way into the vial or salt water after several uses?

What happens is that you may end up injecting bacteria into your body.

Bacteriostatic water with .9% BA is sufficient to kill all bacteria upon contact within 2 days. Studies have been done that demonstrate that sterilizing power.

So IF there is bacteria in the vial or in the powder or in the reconstituting agent itself it will die quickly upon contact with the BW.

So if you reconstitute with BW and you let it sit for 2 days you can pretty much be assured that you will not be injecting live bacteria into your body.

[moose069]

Originally Posted by datBtrue

The average PH is slightly lower but that is of little consequence. There is no worry about the peptide...the worry is with the end user.

Thanks Dat.

So if it takes 20 days to administer a 2mg vial to my rat at 100mcg per day, there's no issues with the reconstituted CJC sitting in the fridge for that period of time?

[datBtrue]

Originally Posted by moose069

Thanks Dat.

So if it takes 20 days to administer a 2mg vial to my rat at 100mcg per day, there's no issues with the reconstituted CJC sitting in the fridge for that period of time?

No. The amino acids used in creating that peptide (i.e. GHF 1-29) are subject to oxidative degradation (unlike those used in GHRP-6) but that won't come into play in your scenario. If kept refrigerated it should be fine for a couple of months.

[RIjeosXeper]

your 300 mcg of CJC per day every day alongside 300 mcg of GHRP-6 idea sounds interesting Dat.. I'm wondering about the degradation time, it would probably keep your CJC levels higher for longer periods of time than 1 mg 2X a week, right ?...

Currently I'm doing 1 mg of cjc 2X a week and 300 mcg of ghrp-6 every day split into 3 shots and I do get slight tingling in my feet and hands, especially when I dose the GHRP-6, but it's not much. Some people talk about unbearable pain in the joints and I just dont feel it. I don't know if my dose is too low or if I should up it, or split my CJC into more shots per week.

According to the "Units" part of your research, 30 mcg/kg (a bit less than what I'm taking right now; I'm taking 27 mcg/ kg) raised your GH levesl more than 10 IU's of HGH a day if I remember correctly. And that's without counting the GHRp-6 and their synergistic effect. 10 IU's seems like a pretty high dose and some people have been complaining about side effects at lower doses... I don't know if it's because different bodies work differently or what but aside from some very slight tingling when I inject the GHRP-6, I have no side effects whatsoever.... I'm still trying to figure out exactly if this is good or bad, got any input ?

[pumbertot]

your 300 mcg of CJC per day every day alongside 300 mcg of GHRP-6 idea sounds interesting Dat.. I'm wondering about the degradation time, it would probably keep your CJC levels higher for longer periods of time than 1 mg 2X a week, right ?...

Currently I'm doing 1 mg of cjc 2X a week and 300 mcg of ghrp-6 every day split into 3 shots and I do get slight tingling in my feet and hands, especially when I dose the GHRP-6, but it's not much. Some people talk about unbearable pain in the joints and I just dont feel it. I don't know if my dose is too low or if I should up it, or split my CJC into more shots per week.

According to the "Units" part of your research, 30 mcg/kg (a bit less than what I'm taking right now; I'm taking 27 mcg/ kg) raised your GH levesl more than 10 IU's of HGH a day if I remember correctly. And that's without counting the GHRp-6 and their synergistic effect. 10 IU's seems like a pretty high dose and some people have been complaining about side effects at lower doses... I don't know if it's because different bodies work differently or what but aside from some very slight tingling when I inject the GHRP-6, I have no side effects whatsoever.... I'm still trying to figure out exactly if this is good or bad, got any input ?

so when you are injecting the 1mg CJC, you dont feel the intense flushing in your head/face? I always do.

[RIjeosXeper]

Originally Posted by pumbertot

so when you are injecting the 1mg CJC, you dont feel the intense flushing in your head/face? I always do.

I do, that's the only time I feel it though. No tiredness, no carpal ts., nothing else. Just intense flushing when I inject the CJC, but after 10 minutes or so I'm fine. Very slight tingling on my hands and feet, but that's about it...

[pumbertot]

Originally Posted by RIjeosXeper

I do, that's the only time I feel it though. No tiredness, no carpal ts., nothing else. Just intense flushing when I inject the CJC, but after 10 minutes or so I'm fine. Very slight tingling on my hands and feet, but that's about it...

i havent run cjc on its own yet so i cant comment on the gh like side-effects. anyone else here that can chime in?

[TripDog]

Originally Posted by RIjeosXeper

I do, that's the only time I feel it though. No tiredness, no carpal ts., nothing else. Just intense flushing when I inject the CJC, but after 10 minutes or so I'm fine. Very slight tingling on my hands and feet, but that's about it...

Yea that seems to be the main thing tha